Method for preparing DL-tryptophan feed additive

A feed additive and tryptophan technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, and animal feed molding or processing, can solve the problems of low fermentation acid production, improve protein synthesis, and improve feed quality. Utilization rate, high effect of animal absorption and utilization rate

Inactive Publication Date: 2017-05-24
CHANGSHA POWERLIFE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some research work has been done on the production of tryptophan by direct fermentation in my country, the level of fermentation acid production is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Breeding and biological characteristics of high-producing bacteria of L-tryptophan

[0024] The starting bacteria Corynebacterium glutamicum LZS-1 and LZS-2 were subjected to ultraviolet, sodium nitrite, diethyl sulfate and nitrosoguanidine mutagenesis and a series of primary and secondary screening to obtain acid-producing L-tryptophan The amount of strain was 38.27g / L. The bacterium is tyrosine auxotroph, showing different levels of 6-fluorotryptophan, 5-methyltryptophan, 5-fluorotryptophan, 4-fluorophenylalanine, sulfaguanidine and cinnamic acid resistance.

[0025] (1), UV mutagenesis

[0026] Under the condition of a fixed UV lamp of 40 watts and a distance of 20 cm, the mutagenesis was performed after 60 s of UV irradiation.

[0027] (2), sodium nitrite mutagenesis

[0028] The mutagenesis concentration of immobilized NaNO2 was 0.03mol / L, after 360s of mutagenesis.

[0029] (3) Mutagenesis of diethyl sulfate (DES)

[0030] With 25%Na 2 8 2 O 3 The aque...

Embodiment 2

[0046] The difference from Example 1 is:

[0047] The weight ratio of each component of the seed medium is: glucose 7, ammonium sulfate 0.35, corn steep liquor 5, yeast extract 1.4, magnesium sulfate 0.02, potassium dihydrogen phosphate 0.25, dipotassium hydrogen phosphate 0.15, pH 7.2;

[0048] The weight ratio of each component of the fermentation medium is: sucrose 5, potassium dihydrogen phosphate 0.25, dipotassium hydrogen phosphate 0.13, magnesium sulfate 0.08, ammonium sulfate 1.0, corn steep liquor 5, ferrous sulfate 0.0013, manganese sulfate 0.0013, sulfuric acid Copper 0.0006, L-tyrosine 0.04, thiamine hydrochloride 0.00004, pH 7.2.

Embodiment 3

[0050] The difference from Example 1 is:

[0051] The weight ratio of each component of the seed medium is: glucose 8, ammonium sulfate 0.4, corn steep liquor 6, yeast extract 1.5, magnesium sulfate 0.03, potassium dihydrogen phosphate 0.3, dipotassium hydrogen phosphate 0.2, pH 7.5;

[0052] The weight ratio of each component of the fermentation medium is: sucrose 5.5, potassium dihydrogen phosphate 0.2, dipotassium hydrogen phosphate 0.14, magnesium sulfate 0.10, ammonium sulfate 0.8, corn steep liquor 5.5, ferrous sulfate 0.0014, manganese sulfate 0.0014, sulfuric acid Copper 0.0006, L-tyrosine 0.035, thiamine hydrochloride 0.00004, pH 7.5.

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Abstract

The invention discloses a method for preparing a DL-tryptophan feed additive. The method comprises the following steps: (1) cultivating a high-yield strain of L-tryptophan; (2) placing the high-yield stable strain into a culture medium, and performing large-scale fermentation culture under set fermentation culture process conditions to obtain an L-tryptophan fermentation culture liquid; (3) extracting L-tryptophan; (4) preparing L-tryptophan mixed feed. The mutation spectrum is designed according to the metabolic pathway of tryptophan, mutation screening is performed by different mutagens, the strain with the L-tryptophan production amount being 39.16 g/L is obtained, and the L-tryptophan fermentation culture liquid is applied to the feed additive. The L-tryptophan feed additive produced in the liquid state has the advantages of low cost, safe quality and high animal absorption and utilization rate, can improve balance of amino acid in livestock and poultry animal feed and increases the feed utilization and protein synthesis.

Description

technical field [0001] The invention belongs to the technical field of tryptophan preparation, in particular to a method for producing L-tryptophan feed additive. Background technique [0002] Amino acid feed additives are mostly imported products because of their important functions and high prices. The first and second limiting amino acids of pigs are lysine and methionine; the first and second limiting amino acids of chicken are just the opposite. While tryptophan is the main limiting amino acid after lysine and methionine, its content is very small in nature, and it has many important physiological functions. Due to the fact that a large amount of lysine and methionine are added to the compound feed in my country, the tryptophan is in a state of relative shortage, and the demand is on the rise; The price is high, and it currently needs to be imported in large quantities. [0003] The production methods of tryptophan mainly include the early chemical synthesis method an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/01C12P13/22A23K40/00A23K20/142A23K10/10C12R1/15
CPCC12N15/01C12P13/227C12N1/205C12R2001/15
Inventor 刘继根
Owner CHANGSHA POWERLIFE BIOTECH
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