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Preparation method of SBA-15 immobilized angiotensin converting enzyme

An angiotensin-converting enzyme technology, applied in biochemical equipment and methods, immobilized on or in inorganic carriers, enzymes, etc., can solve the problem of low activity of angiotensin-converting enzyme, poor stability, immobilization Low efficiency and other problems, to achieve the effect of improving enzyme activity and adsorption rate, good product stability, and uniform surface properties

Active Publication Date: 2017-05-24
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional carriers for immobilizing ACE include agarose, chitosan, silicon dioxide, chitosan, etc. The activity of ACE after immobilization is low, and the immobilization efficiency is low, and the stability is not very good. Good, but also more expensive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1) Preparation of a mesoporous molecular sieve SBA-15 carrier, the specific preparation method process is as follows:

[0034]Preparation of mesoporous molecular sieve SBA-15 carrier: Dissolve 1g of polyethylene oxide-polypropylene oxide-polyethylene oxide triblock copolymer (P123) in 50mL of 1.6mol / L hydrochloric acid solution and stir in a water bath at 30°C 3h, then add 5mL of tetraethyl orthosilicate (TEOS) dropwise, and keep stirring in a water bath at 40°C for 20h; then transfer the reactant to a reaction kettle, put it in an oven at 110°C and let it stand for 24h; finally collect the solid The product, washed with water and suction filtered, was placed in an oven at 80°C for 12 hours, then taken out and placed in a muffle furnace at 550°C for 8 hours to obtain mesoporous molecular sieve SBA-15; :ZnSO 4 (0.05mol / L of ZnSO 4 solution)=1:10 (g / mL) added to ZnSO 4 Solution, 35°C constant temperature water bath shaking pot shaking reaction for 50min; after 50min, t...

Embodiment 2

[0044] 1) Preparation of mesoporous molecular sieve SBA-15 carrier: Take 1g polyethylene oxide-polypropylene oxide-polyethylene oxide triblock copolymer (P123) and dissolve it in 50mL 1.6mol / L hydrochloric acid solution at 30°C Stir in a water bath for 3 hours, then add 5 mL of tetraethyl orthosilicate (TEOS) dropwise, and continue stirring in a water bath at 40°C for 20 hours; then transfer the reactant to a reaction kettle, and put it in an oven at 110°C to react for 24 hours; Finally, collect the solid product, wash it with water and filter it, put it in an oven at 80°C for 12 hours, take it out and put it in a muffle furnace at 550°C for 8 hours to obtain mesoporous molecular sieve SBA-15; add 0.5g of SBA-15 to 200ml of absolute ethanol and then add 12mL of 3-(methacryloyloxy)propyltrimethoxysilane, shake in a water bath at 30°C for 15h, suction filter after the reaction, wash and connect with IDA, add 0.7mol / L IDA at a ratio of 1:10 (g / mL) Solution (IDA solution is prepar...

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PUM

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Abstract

The invention provides a preparation method of an SBA-15 immobilized angiotensin converting enzyme (ACE). A mesoporous molecular sieve SBA-15 chelated with zinc ions is used as a carrier and an angiotensin converting enzyme is used as an immobilization object, and the carrier and the immobilization object are subjected to adsorption and bonding effects to obtain the SBA-15 immobilized angiotensin converting enzyme. In a product obtained by the method, the ACE is immobilized in pore channels of the zinc ion chelated mesoporous molecular sieve SBA-15 and an outer surface of the molecular sieve. According to the preparation method, an immobilization process for preparing the Zn-SBA-15 immobilized ACE is simple and the stability of the product is good; and compared with a free enzyme, the prepared Zn-SBA-15 immobilized ACE has better temperature and pH (Potential of Hydrogen) stability.

Description

technical field [0001] The invention relates to the technical field of immobilized enzyme preparation, in particular to a method for applying chelated Zn 2+ Preparation of Mesoporous Molecular Sieve SBA-15 Immobilized Angiotensin Converting Enzyme. Background technique [0002] High blood pressure can change and damage the structure and function of the brain, heart, blood vessels, kidneys, and fundus of the eye, causing concurrent diseases of these organs and leading to functional failure. The following complications caused by high blood pressure: coronary heart disease, cerebrovascular disease, hypertensive heart and brain disease, chronic renal failure and atherosclerosis, etc. The current antihypertensive drugs mainly include diuretics, β receptor blockers, calcium channel blockers, angiotensin converting enzyme (ACE) inhibitors and angiotensin II receptor blockers. [0003] Angiotensin-converting enzyme (ACE) is a multifunctional dipeptide carboxypeptidase present in d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N9/48
CPCC12N9/485C12N11/14C12Y304/17023
Inventor 付颖寰孙美玲宋铖铖张钦年益营马倩侯永涛
Owner DALIAN POLYTECHNIC UNIVERSITY
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