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Colorless and transparent silk sericin hydrogel, stent prepared therefrom and application thereof

A technology of sericin and hydrogel, applied in biochemical equipment and methods, cell culture support/coating, tumor/cancer cells, etc.

Inactive Publication Date: 2017-05-24
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

However, there is currently no supporting material that can construct 3D tumor models in vitro and is suitable for observation, tracking and research of such tumor models

Method used

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  • Colorless and transparent silk sericin hydrogel, stent prepared therefrom and application thereof
  • Colorless and transparent silk sericin hydrogel, stent prepared therefrom and application thereof
  • Colorless and transparent silk sericin hydrogel, stent prepared therefrom and application thereof

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Embodiment Construction

[0051] The principles and features of the present invention are described below in conjunction with the examples and accompanying drawings, and the given examples are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0052] Preparation of Sericin Hydrogels and Hydrogel Scaffolds

[0053] Sericin hydrogels and hydrogel scaffolds of the present invention are prepared by the following methods:

[0054] 1. Preparation of sericin aqueous solution

[0055] Silk was obtained from cocoons of Bombyx mori silk fibroin-deficient mutants (purchased from the Institute of Sericulture, Chinese Academy of Agricultural Sciences). An aqueous solution of sericin was prepared from the cocoons by the following method:

[0056] 1) Weigh 1 g of silkworm cocoon of mutant silkworm and cut it into 1 cm 2 The fragments were placed in a clean beaker, washed 3 times with ultrapure water, and centrifuged at 3500rpm for 5 minutes to remove wat...

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Abstract

The invention relates to a colorless and transparent sericin hydrogel, which is prepared by utilizing HRP and H2O2 to catalyze silk sericin cross-linking. The invention also provides application of the silk sericin hydrogel in biomedical materials. The invention also provides a stent obtained by freeze-drying of the silk sericin hydrogel. The invention also provides the role of the stent in in-vitro construction of a three-dimensional tumor model, in-vitro construction of a cell culture model, study of drug resistance of tumors, and screening of new drugs. The silk sericin hydrogel provided by the invention and the stent prepared therefrom can be used for carrying cells, especially tumor cells, and establishing a three-dimensional tumor model, and because of the colorless and transparent characteristics, the sericin hydrogel and the stent provided by the invention are conducive to observation and tracing of the constructed three-dimensional tumor model, thus better studying tumor cells and the development biological behaviors of tumor tissues.

Description

technical field [0001] The present invention relates to the field of tissue engineering materials, and more particularly relates to a colorless and transparent sericin hydrogel, a scaffold prepared therefrom and applications thereof. Background technique [0002] Hydrogel (Hydrogel) is a gel with water as the dispersion medium. There are hydrophobic groups and hydrophilic groups in the water-soluble polymer with network crosslinked structure, and the hydrophilic groups combine with water molecules to bind water molecules It is a cross-linked polymer that is connected inside the network and the hydrophobic groups swell with water. [0003] Sericin (Silk Sericin) is a natural macromolecular viscous protein wrapped on the surface of silk fibers, accounting for about 20-30% of the cocoon content, composed of polypeptides with a molecular weight of 24-400kDa, and its molecules are composed of serine, asparagus It is composed of 18 kinds of amino acids such as amino acid and glyc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08L89/00C12N5/09
CPCC08J3/245C08J2389/00C12N5/0693C12N2533/50
Inventor 王征王琳王健李晓麟
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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