Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Tomato 212 SNP loci as well as applications thereof to identification of variety authenticity and seed purity of Lycopersicon esculentum

A technology of authenticity and variety, applied in the biological field, it can solve the problems of low polymorphism, small genetic variation of tomato, and few types of enzymes.

Active Publication Date: 2017-05-17
北京通州国际种业科技有限公司 +1
View PDF1 Cites 19 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The isoenzymes used for the identification of tomato variety seed purity mainly include POD, EST, ACP, ADH, MDH, etc. It is related to the development period, and it is difficult to use some F 1 Variety Identification and Purity Testing of Hybrids

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tomato 212 SNP loci as well as applications thereof to identification of variety authenticity and seed purity of Lycopersicon esculentum
  • Tomato 212 SNP loci as well as applications thereof to identification of variety authenticity and seed purity of Lycopersicon esculentum
  • Tomato 212 SNP loci as well as applications thereof to identification of variety authenticity and seed purity of Lycopersicon esculentum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Embodiment 1, the preparation of the primer set that is used to detect 212 SNP site genotypes

[0074] (1) Obtain information on 7,000 SNP sites covering 12 tomato chromosomes by searching literature (Sim SC, et al. (2012) High-Density SNP Genotyping of Tomato (Solanum lycopersicum L.) Reveals Patterns of Genetic Variation Due to Breeding . Plos One 7(9): e45520-e45520.).

[0075] (2) 212 SNP sites were selected from the above SNP sites, and the physical positions and flanking sequences of these 212 SNP sites were determined based on the whole genome sequence (version number SL2.50) of tomato variety Heinz 1706 Yes, the 212 SNP sites are numbered 001-212, and the 212 SNP sites are specifically shown in Table 1.

[0076] Table 1, 212 SNP site information

[0077]

[0078]

[0079]

[0080]Three primers are required to detect the deoxynucleotide of each SNP site, two upstream primers (denoted as P1 and P2) and one downstream primer (denoted as P3). The detecti...

Embodiment 2

[0082] Example 2. Using a set of primers for detecting the genotypes of 212 SNP sites to identify the authenticity of tomato varieties

[0083] (1) The tomato material for constructing the map was provided by Mr. Li Changbao from the Vegetable Center of Beijing Academy of Agriculture and Forestry Sciences. From 402 materials, 100 varieties were randomly selected (Table 2).

[0084] Table 2, 100 test tomato varieties

[0085]

[0086]

[0087] (2) Extract the DNA of the tested tomato varieties

[0088] Using the CTAB method to extract the DNA of the tested variety: take 20mg-30mg of young tomato leaves into a 2.0mL centrifuge tube, grind in a grinder for 10min; add 750μL of preheated CTAB extract, shake well, and put in a water bath at 65°C for 45min; add 750 μL of chloroform, mix up and down for 3 minutes, centrifuge at 12,000 g for 5 min; pipette 500 μL of supernatant into another 2.0 mL centrifuge tube, add 0.7 times the volume of pre-cooled isopropanol, invert the ce...

example 1：Allele1/Allele1，A/C，Allele1A，A/A。 example 2

[0099] According to the data read by the instrument's own software, the data obtained by the above two detection platforms are analyzed to form the fingerprint of each tomato variety. The genotype data of the homozygous site is recorded as Allele1 / Allele1 or Allele2 / Allele2, and the genotype data of the heterozygous site is recorded as Allele1 / Allele2, where Allele1 and Allele2 are the two alleles on the mutation site; The genotype data of missing loci were recorded as 0. Example 1: The genotype of a sample at a certain site is Allele1 / Allele1, the allele at this site is A / C, Allele1 represents base A, and the genotype at this site is recorded as A / A. Example 2: The genotype of the sample at a certain site is Allele1 / Allele2, the allele at this site is A / C, Allele1 represents base A, Allele2 represents base C, and the genotype at this site is recorded for A / C.

[0100] Determine whether the tomato to be tested is the same variety according to the test results: when the numbe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses tomato 212 SNP loci as well as applications thereof to identification of variety authenticity and seed purity of Lycopersicon esculentum. In the applications, physical locations of 212 SNP loci are determined based on whole genome sequence alignment of Lycopersicon esculentum variety Heinz 1706, and the version number of a whole genome sequence of Lycopersicon esculentum variety Heinz 1706 is SL2.50. 212 SNP loci are used for establishing a Lycopersicon esculentum DNA fingerprint identification standard experimental system, which is rapid and accurate, economic and simple, stable and reliable, and on the basis, a method which is suitable for establishing a Lycopersicon esculentum finger-print bank, and identifying variety authenticity and seed purity. The method for identifying variety authenticity and seed purity of Lycopersicon esculentum, and has the advantages of fully improved experimental efficiency in time, rapid, accuracy, convenient operation, and the like; and the identification results are not influenced by environments.

Description

technical field [0001] The invention relates to 212 SNP sites of tomato and its application in identifying tomato variety authenticity and seed purity in the field of biotechnology. Background technique [0002] Tomato (Lycopersicon esculentum L.) is one of the main crops of protected vegetable production in my country. It is also the vegetable crop with the largest planting area and high output value in Beijing. It plays an important role in ensuring the supply of vegetable baskets for the citizens of the capital. Due to the unique edible, health care and auxiliary therapeutic effects of tomato and its products, it occupies an important position in the world's agricultural production and trade. According to statistics released by the World Processed Tomato Council (WPTC): In 2013, the global output of processed tomatoes was 33.012 million tons. Along with the advantageous development of my country's tomato seed industry, some decked varieties appeared on the market, shoddy...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 刘宝平王喜萍李常保王铭堂张东峰王亚楠马俊召周明刘海燕杜敏敏
Owner 北京通州国际种业科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com