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An automatic microscopic imaging system for multi-cell culture process

A technology for cell culture and microscopic imaging, which is used in microscopes, enzymology/microbiology devices, and material analysis by optical means to improve experimental efficiency, reduce experimental costs, and improve imaging quality.

Inactive Publication Date: 2011-12-21
BEIHANG UNIV
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Problems solved by technology

[0003] The technical problem solved by the present invention is to overcome the deficiencies of the prior art and provide an automatic microscopic imaging system, which considers the problems of low power consumption, automatic focus and tracking imaging in the imaging process, and realizes automatic real-time imaging in the process of cell culture And cell growth state analysis, can be used in some unattended special cell culture environment

Method used

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  • An automatic microscopic imaging system for multi-cell culture process
  • An automatic microscopic imaging system for multi-cell culture process
  • An automatic microscopic imaging system for multi-cell culture process

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Embodiment Construction

[0031] Such as figure 1 As shown, the measurement device of the present invention includes a light source system 1 , an optical mechanism 2 , a building-type multilayer cell culture observation mechanism 3 , a multi-axis servo system 4 , and an automatic culture imaging system control mechanism 5 . Among them, such as image 3 As shown in (a), the center of the light source system and the center of the optical component are installed at the coaxial position of the Z axis, such as figure 2 Shown is the design and lighting mode of the light source system 1. The programmable LED array 101 provides selectable lighting areas and lighting intensity in the field of view to achieve low power consumption of lighting. The light is converted into collimated light having a diameter suitable for coupling to the large area stationary optic 103; thereafter passed through the large area stationary optic 103, including small angle diffusers or beam homogenizers, improving collimation. Spati...

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Abstract

An automatic microscopic imaging system for multi-cell culture process, which is used for automatic imaging and analysis of long-term cell culture process. The system has the functions of automatic observation target switching, automatic low-power light source lighting, auto-focus and image fusion, intelligent online analysis of cell growth status, and tracking imaging of interested imaging regions and moving cells. Including light source system, optical mechanism, building-type multi-layer cell culture observation mechanism, multi-axis servo system, automatic culture imaging system control mechanism, etc., can realize simultaneous cultivation and imaging of various cells without human participation for a long time.

Description

technical field [0001] The invention relates to an automatic cell imaging system, which belongs to the technical field of microscopic imaging in biomedicine, in particular to automatic imaging in the process of cell culture and real-time measurement and analysis of the cell state in the culture process. Background technique [0002] Cells constitute the basic unit of life and reflect the essential laws of life. So the study of cell biology provides the backbone for the study of other biology, including genetic biology; muscle, bone, and mineral metabolism; cardiorespiratory and other adaptive systems; immunology; and motor-sensory coordination. Research at the tissue and organismal level in each field is ultimately determined by the normal function of individual cells and the physiological networks formed by their fusion. Advanced cell culture techniques have made it possible to study certain cells individually at the cellular level. In recent years, cell culture technolog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B21/36G02B21/06G01N21/17C12M1/00C12M1/38C12M1/36
Inventor 樊尚春夏伟强邢维巍
Owner BEIHANG UNIV
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