Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of apigenin in preparation of drugs for preventing and treating renal fibrosis

A technology of kidney fibrosis and apigenin, applied in the field of medicine, can solve the problems of prolonging the life of patients and unsolved fundamental problems, and achieves the effect of treating kidney fibrosis, improving kidney fibrosis, and having obvious effect.

Inactive Publication Date: 2017-05-10
THE THIRD AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIV OF PLA
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

7. End-stage renal disease can choose blood, peritoneal dialysis or transplantation to prolong the life of the patient, but the fundamental problem has not been solved in the end
However, apigenin is used to treat renal fibrosis, and there are no reports in domestic and foreign literature.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of apigenin in preparation of drugs for preventing and treating renal fibrosis
  • Application of apigenin in preparation of drugs for preventing and treating renal fibrosis
  • Application of apigenin in preparation of drugs for preventing and treating renal fibrosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1D

[0051] Example 1 DOCA-salt-induced renal fibrosis animal model

[0052] 1. DOCA-salt-induced renal fibrosis animal model:

[0053] Twenty-four healthy 2-month-old male SD rats (provided by the Animal Experiment Center of Daping Hospital, Third Military Medical University), weighing 250-300 g, were housed in separate cages and allowed to eat and drink freely. Natural day and night lighting, room temperature 18°C-26°C, feeding in the morning and evening every day, changing water every other day. Humidity around 50%. They were adaptively fed for 1 week before the operation, and then the left nephrectomy was performed. Rats were weighed and anesthetized by intraperitoneal injection of 10% urethane solution (1m1 / kg), then the back operation area was sheared, fixed on the operation board in the prone position, and after strict disinfection, a test was made on the left side of the spine and the lower edge of the twelfth rib. An incision about 2 cm in length was made to separate th...

Embodiment 2

[0058] Embodiment 2 animal experiments

[0059] 1. Determination of body weight: common weighing method.

[0060] 2. Determination of urinary albumin and creatinine, the results are as follows: Image 6 shown.

[0061] 2.1 Collection of 24-hour urine samples

[0062] After the dietary intervention, the rats were placed in metabolic cages in the morning, fed in separate cages, 1 rat per cage, fasted, and free to drink water. Natural lighting day and night, room temperature 18°C-26°C, humidity around 50%. Feed was added by special personnel, and the weight of the feed was weighed regularly with a balance every day, and the daily food intake of each rat was calculated. Collect 24-hour urine volume, centrifuge at 4000r / min at room temperature for 10min, and store in -70°C refrigerator for later use.

[0063] 2.2 Reagent preparation, usage and storage

[0064] Alb. Standards: Add 0.5ml of buffer solution to dissolve each standard, shake well after 15 minutes of dissolution. ...

Embodiment 3

[0116] Embodiment 3 molecular experiment

[0117] 1. Western blot, the results are as follows Figure 10 , Figure 11 and Figure 12 shown.

[0118] 1.1 Tissue and cell total protein extraction method

[0119] 1.1.1 Take 100mg of tissue and add appropriate amount of pre-cooled RIPA lysate (1ml protein homogenization buffer plus PMSF50ug, Leuptin 5ug), homogenize with a homogenizer, collect the homogenate in a 1.5ml centrifuge tube; if it is a cell sample, Add an appropriate amount of lysate directly to the culture flask or six-well plate, and collect the cells with a cell scraper.

[0120] 1.1.2-20°C for 20 minutes to precipitate protein.

[0121] 1.1.3 Centrifuge at 12,000 rpm at 4°C for 20 minutes, collect the supernatant, namely histoprotein, for protein quantification and immunoblotting experiments, or freeze at -70°C for later use.

[0122] 1.1.4 Protein quantification method (Bradford method)

[0123] 1.1.5 Preparation of protein standard solution: Prepare 1 mg / ml...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to application of apigenin in the preparation of drugs for preventing and treating renal fibrosis. The drugs are used to treat early fibrosis and improve progress of renal fibrosis, have no significant adverse effects, are good in safety with low toxic and side effects, and are particularly suitable for patents with early fibrosis.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the use of apigenin in the preparation of medicines for preventing and treating renal fibrosis. Background technique [0002] Renal fibrosis is a common change in the development of all kidney diseases to end-stage renal failure, and all chronic kidney diseases can eventually develop into renal fibrosis. Its main pathogenesis is as follows: in the process of renal fibrosis, cell changes will activate fibroblasts and mesangial cells, promote the infiltration of T lymphocytes, monocytes or macrophages, and the transformation of tubular epithelial cells into mesangial cells ( EMT). A variety of infiltrating cells and self-cells interact and participate in the formation of renal fibrosis. When the kidney is damaged, a series of repair reactions will occur in its tissue, and a large amount of ECM will be produced, and the activated ECM effector cells are usually the core of renal fibrosis. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P13/12
CPCA61K31/352
Inventor 祝之明刘道燕魏星
Owner THE THIRD AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIV OF PLA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com