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Preparation method of fruit tree biological insecticide

A biopesticide and fruit tree technology, applied in botany equipment and methods, insecticides, biocides, etc., can solve problems affecting crop growth environment and human and animal health, loss of insecticidal effect, and pest resistance. Achieve the effects of low cost, simple preparation steps and broad application prospects

Inactive Publication Date: 2017-05-10
孙祎
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention: After long-term use of traditional fruit tree insecticides, the pests will develop drug resistance, lose the insecticidal effect, and the residual poison period is long, which seriously affects the crop growth environment and the health of humans and animals.
The present invention uses plant extracts to destroy the synthesis of proteins in pests, so as to obtain chitin formed by enzymatically decomposing pests, and prevent the process of peeling and metamorphosis of pests, so that they cannot peel and die, thus effectively solving the long-term use of traditional fruit tree pesticides Afterwards, it will cause the pests to develop drug resistance, lose the insecticidal effect, and the residual poison period is long, which seriously affects the crop growth environment and the health of humans and animals. The effect is good, and it does not contain toxic substances, and will not affect the growth of crops and the health of humans and animals

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0023] First take the soil at a distance of 0.8 cm from the main root of the fruit tree, mix it with a 6% glucose solution that is 1.2 times its mass, put it in a container, and place the container at 32°C for 12 hours, every 40 minutes, Oxygenate the mixture in the container from the bottom of the container, the oxygenation speed is 4mL / min, and the oxygenation time is 2min; The mixture in the container was stirred with 150r / min for 6min, and the mixture in the container was filtered with 50 mesh gauze, and the filtrate was collected to obtain a mixed bacterial suspension for subsequent use; in parts by weight, 55 parts of sterile water, 23 parts of peptone, 20 parts of yeast extract, 14 parts of glucose, 6 parts of tyrosine, 4 parts of lecithin and 3 parts of dipotassium hydrogen phosphate, put them into a blender and stir evenly, and put the stirred mixture into a high-temperature sterilization pot Sterilize to obtain nutrient solution; according to the volume ratio of 1:6,...

example 2

[0026] First take the soil at a distance of 0.4 cm from the main root of the fruit tree, mix it with a 6% glucose solution with a mass fraction of 0.9 times its mass, put it in a container, and place the container at 25°C for 10 hours, every 30 minutes, Oxygenate the mixture in the container from the bottom of the container, the oxygenation speed is 4mL / min, and the oxygenation time is 1min; The mixture in the container was stirred with 150r / min for 4min, and the mixture in the container was filtered with 50 mesh gauze, and the filtrate was collected to obtain a mixed bacterial suspension for subsequent use; in parts by weight, 50 parts of sterile water, 20 parts of peptone, 18 parts of yeast extract, 12 parts of glucose, 3 parts of tyrosine, 2 parts of lecithin and 1 part of dipotassium hydrogen phosphate, put them into a blender and stir evenly, and put the stirred mixture into a high-temperature sterilization pot Sterilize to obtain nutrient solution; according to the volum...

example 3

[0029] First take the soil at a distance of 0.6 cm from the main root of the fruit tree, mix it with a 6% glucose solution that is 1.1 times its mass, put it in a container, and place the container at 30°C for 11 hours, every 35 minutes, Oxygenate the mixture in the container from the bottom of the container, the oxygenation speed is 4mL / min, and the oxygenation time is 2min; The mixture in the container was stirred with 150r / min for 5min, and the mixture in the container was filtered with 50 mesh gauze, and the filtrate was collected to obtain a mixed bacterial suspension for subsequent use; in parts by weight, 52 parts of sterile water, 22 parts of peptone, 19 parts of yeast extract, 13 parts of glucose, 5 parts of tyrosine, 3 parts of lecithin and 2 parts of dipotassium hydrogen phosphate, put them in a blender and stir evenly, and put the stirred mixture into a high-temperature sterilization pot Sterilize to obtain nutrient solution; according to the volume ratio of 1:4, m...

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Abstract

The invention relates to a preparation method of a fruit tree biological insecticide, belonging to the technical field of insecticide preparation. In the invention, the soil at the fruit tree taproot is used as a bacteria source, and the harm to the fruit tree is avoided; the quantity of aerobic bacteria is increased by oxygen. The preparation method comprises the following steps: screening the aerobic bacteria by use of veratryl alcohol to obtain target mixed bacteria; performing optimized screening culture of the mixed bacteria; with tyrosine and lecithin as screening medium, screening the target mixed bacteria; with pest peeling powder as an induction nutrient, performing induction culture of the bacteria to obtain an enzyme for degrading pest peel; and then mixing with a plant extract to obtain the fruit tree biological insecticide. In the invention, the efficacy of the prepared fruit tree biological insecticide lasts for 40-45 days; after long-term use, drug resistance of the pests is avoided, the insecticidal rate can reach 98% or exceed 98%, and the insecticidal effect is relatively good; and without toxic substances, the fruit tree biological insecticide does not harm the health of crops and human body and meets the requirement for sustainable development.

Description

technical field [0001] The invention relates to a preparation method of a biological insecticide for fruit trees, belonging to the technical field of insecticide preparation. Background technique [0002] As one of the eight origin centers of cultivated plants in the world, my country has a long history of cultivation, rich resources, and a wide variety of varieties. Fruit trees have become an important part of my country's crop industry. However, fruit trees are very frequent and serious pests First, there are many types of fruit trees, complex varieties, and perennial plants, which provide a relatively stable ecological environment for the occurrence of pests and become a favorable place for the reproduction and outbreak of pests. A series of changes and damages occurred in the organizational structure, which seriously affected the fruit quality and yield. With the change of agricultural ecological environment, the adjustment of crop layout and industrial structure, the ren...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/34A01N63/00A01P7/04
CPCA01N65/00A01N63/00A01N65/34A01N2300/00
Inventor 孙祎薛红梅宋家平
Owner 孙祎
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