Three-channel SPR (surface plasma resonance) sensor based on seven-core optical fiber

Abstract

The invention provides a three-channel SPR (surface plasma resonance) sensor based on a seven-core optical fiber. The three-channel SPR sensor comprises a supercontinuum light source, a light injection optical fiber, a seven-core optical fiber coupling device, the seven-core optical fiber, a seven-core optical fiber SPR sensing probe, a receiving optical fiber, a spectrometer, a micro-injection pump, a microfluidic chip and a waste fluid pool, wherein source light is fed into the light injection optical fiber and is then injected into a light injection core of a pair of symmetrical cores of the seven-core optical fiber; switching between three groups of symmetrical fiber cores of the seven-core optical fiber can be realized by a precision rotator of the coupling device; light is subjected to three times of total reflection at symmetrical cone angle structures of the SPR probe at an end part of the seven-core optical fiber and subjected to SPR; reflected light of the probe enters a reflection core of the group of fiber cores, enters the receiving optical fiber through the coupling device and is fed into the spectrometer, an echo attenuation spectrum is collected, a to-be-detected solution is injected into the microfluidic chip by the micro-injection pump, and a waste fluid obtained after measurement flows into the waste fluid pool. The three-channel SPR sensor based on the seven-core optical fiber can realize multi-channel detection and can solve the problem about simultaneous detection of multiple analytes in a mixture in the same sensing area. An SPR segmented detection technology is realized by three groups of cone angle structures with different grinding angles of the seven-core optical fiber, and ultrasensitive SPR segmented optical fiber sensor can be constructed and has very good application prospect in the fields of biology, chemistry and medical treatment.

Description

technical field [0001] The invention belongs to the field of SPR sensors, in particular to a three-channel SPR sensor based on a seven-core optical fiber. Background technique [0002] Surface plasmon resonance (SPR) biosensors have been increasingly used for biomolecular interaction, chemical and biological analyte reaction detection. In these fields, SPR biosensors can perform real-time sensitive label-free measurement of the interaction between analytes (e.g. antigens, DNA) and specific binding partners (e.g. antibodies, complementary DNA) immobilized on SPR sensor metal membranes . SPR biosensors can detect changes in the refractive index resulting from analyte binding. However, it is impossible to effectively distinguish the refractive index change caused by specific binding and non-specific binding, and the refractive index change caused by analyte binding and the background medium (sample) refractive index change cannot be distinguished. In order to compensate for ...

AI Technical Summary

Problems solved by technology

However, due to the difficulty in adjusting the resonance range, there will be defects for specific detection channels

Images