New uses of semen persicae
A technology of peach kernels and uses, which is applied in the field of medicine, can solve the problem of not finding tumor neovascularization, etc., and achieve a significant effect and the effect of inhibiting tumor neovascularization
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[0043] 1. Materials and methods
[0044] 1.1 Material
[0045] Chinese medicines were purchased from Sichuan Huichuntang Pharmaceutical Co., Ltd. All reagents were purchased from Sigma-Aldrich Co. (St. Louis, MO), and PTK787 (vatalanib) was purchased from Selleckchem. Fli1a-EGFP transgenic zebrafish was used for experiments. Zebrafish are reared in a standard environment.
[0046] 1.2 Reagent preparation
[0047] Weigh 450g of traditional Chinese medicine, decoct in 8 times the amount of water for 3 times, 1 hour each time, combine the 3 times of decoction on an 800W electric furnace and concentrate to about 400ml, pass through a 100-mesh sieve (filter residue sample), and apply evenly In the evaporating dish, place the evaporating dish in an electric heating blast drying oven, and after intermittent drying at 60°C for 62 hours, continue to dry in a glass desiccator at room temperature until the moisture content is reached <5% (determined by drying method) of extract.
[0048] 1.3 E...
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