Method for identifying different varieties of Anoectochilus roxburghii based on fingerprinting

A technology of fingerprint and clematis, which is applied in the field of high performance liquid chromatography, can solve problems such as limitations and difficulty in distinguishing

Inactive Publication Date: 2017-03-29
SHAOWU FORESTRY SCI TECH PROMOTION CENT
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for identifying different types of clematis based on fingerprints, aiming to solve the problem that the existing identification of clematis is only limited to the plant form, and it is difficult to distinguish them based on experience

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  • Method for identifying different varieties of Anoectochilus roxburghii based on fingerprinting
  • Method for identifying different varieties of Anoectochilus roxburghii based on fingerprinting
  • Method for identifying different varieties of Anoectochilus roxburghii based on fingerprinting

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Embodiment Construction

[0020] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0021] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0022] Such as figure 1 As shown, the method for identifying different types of Clematis clematis based on fingerprints provided by the embodiments of the present invention includes the following steps:

[0023] S101: Weigh 0.20g of the clematis medicinal material powder passed through a 90-mesh sieve into a 5ml centrifuge tube, add 1ml of 80% methanol precisely with a pipette gun, mix well in a vortex oscillator, and put it into a 60°C, 100W ultrasonic extraction 30min, put it at ro...

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Abstract

The invention discloses a method for identifying different varieties of Anoectochilus roxburghii based on fingerprinting; by establishing fingerprinting of Anoectochilus roxburghii through HPLC (high performance liquid chromatography) and qualitatively and quantitatively analyzing three ingredients of Anoectochilus roxburghii, it is possible to quick understand the quality of Anoectochilus roxburghii products, and uniformizing the quality standards for Anoectochilus roxburghii products is facilitated. In the method, the similarities of different batches of Anoectochilus roxburghii are analyzed and compared, peak areas of common peaks of different varieties of Anoectochilus roxburghii are compared, the peak areas of the common peaks and non-common peaks of Anoectochilus roxburghii cultivated in different modes are subjected to comparison and clustering analysis, Anoectochilus roxburghii planted in different modes are identified through the comparison of characteristic peaks, the three ingredients of Anoectochilus roxburghii are subjected to qualitative and quantitative analysis, and uniformizing the quality standards for Anoectochilus roxburghii products is facilitated.

Description

technical field [0001] The invention belongs to the technical field of high-performance liquid chromatography, and in particular relates to a method for identifying different types of Aurantia clematis based on fingerprints. Background technique [0002] At present, my country only involves literature research, distribution status, biological characteristics investigation, commodity investigation, tissue culture, cultivation, etc. for A. clematis in China. There are few establishment of fingerprints of A. clematis by HPLC. A certain part of clematis is limited, and there is no comprehensive and systematic research on the HPLC fingerprints of clematis on imitation ecology. The establishment of the HPLC fingerprints of clematis and the active ingredients Qualitative and quantitative analysis, comparison of clematis in different cultivation methods, and finding the most suitable cultivation method to replace wild clematis have not been reported yet. As a precious Chinese herba...

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 张海燕陈吓俤邹智岳杨开兴王涵林立洪伟祥陈锦秀
Owner SHAOWU FORESTRY SCI TECH PROMOTION CENT
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