Cycli-dependent kinase 6 (CDK6) gene exon genetic groups used for cancer diagnosis and diagnosis kit

A technology of cancer diagnosis and kit, which is applied in the direction of recombinant DNA technology, microbial measurement/testing, DNA/RNA fragments, etc., and can solve problems such as differences in overexpression levels

Inactive Publication Date: 2017-03-29
THE FIRST AFFILIATED HOSPITAL OF XIAMEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Selective replication of DNA within cells, resulting in a large number of copies, which may eventually lead to overexpression of gene products, which varies with tumor type

Method used

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  • Cycli-dependent kinase 6 (CDK6) gene exon genetic groups used for cancer diagnosis and diagnosis kit
  • Cycli-dependent kinase 6 (CDK6) gene exon genetic groups used for cancer diagnosis and diagnosis kit
  • Cycli-dependent kinase 6 (CDK6) gene exon genetic groups used for cancer diagnosis and diagnosis kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Example 1: Fluorescent quantitative PCR method to detect the amplification of CDK6 gene Exon 1 in different cancer tissues (intestinal cancer and breast cancer) and corresponding normal tissues adjacent to cancer (intestine and breast)

[0096] 1. Collect 1 pair of colon cancer and breast cancer tissues and corresponding paracancerous bowel and breast normal tissues. All tissue samples are provided by the Specimen Bank of the First Affiliated Hospital of Xiamen University. Each patient specimen has a clear diagnosis record , and with the consent of the ethics committee, the specimens were collected and frozen in liquid nitrogen.

[0097] 2. DNA sample preparation

[0098] (1) Sample pretreatment: Take out the sample from the liquid nitrogen tank, put 30mg of the sample into a 2ml sterile EP tube, add 600μL of DNA extraction solution and 20mg / ml proteinase K, fully cut it into pieces with sterilized scissors, and then 65 Incubate for 1 hour at °C.

[0099] (2) Centrifu...

Embodiment 2

[0110] Example 2: Fluorescent quantitative PCR method to detect the amplification of CDK6 gene Exon 1-8 in 5 pairs of breast cancer tissues and corresponding adjacent normal tissues

[0111] 1. Collect 5 pairs of breast cancer tissue and corresponding normal adjacent tissue samples. All tissue samples are provided by the Specimen Bank of the First Affiliated Hospital of Xiamen University. Each patient sample has a clear diagnosis record and has been approved by the ethics committee , collected specimens and frozen in liquid nitrogen.

[0112] 2. DNA extraction and fluorescent quantitative PCR methods are the same as in Example 1.

[0113] 3. Data analysis: the experiments were repeated 3 times, and relative quantification 2 -△△Ct (△△Ct=(cancer tissue CDK6Exon1-8Ct value-cancer tissue Exon1-8GAPDH Ct value)-(normal tissue CDK6Exon1-8Ct value-normal tissue Exon1-8GAPDH Ct value)) method for statistical analysis, GAPDH as an internal reference gene, The data were analyzed using...

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PUM

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Abstract

The invention relates to a set of cycli-dependent kinase 6 (CDK6) gene exon genetic groups used for cancer diagnosis, application thereof, and a cancer diagnosis kit based on the CDK6 gene exon genetic groups. A novel molecular cancer diagnostic indicator, namely CDK6 gene exon amplification, is discovered; and application of the CDK6 gene exon genetic groups for preparing the cancer diagnosis kit, and the cancer diagnosis kit are provided. Compared with a traditional detecting method, diagnosis through a molecular marker is more timely and more specific, and accordingly the five-year survival rate of a cancer patient can be increased, the death rate is decreased, and the application prospects are wide.

Description

[0001] (1) Technical field [0002] The invention relates to a CDK6 gene exon gene group used for cancer diagnosis and reference thereof, and a cancer diagnosis kit based on the CDK6 gene exon gene group. [0003] (2) Background technology [0004] According to the statistics of the World Health Organization, the mortality rate of cancer has surpassed coronary heart disease and stroke to become the chronic disease with the highest mortality rate, seriously threatening human health. Worldwide, in 2012, there were 14.1 million new cancer cases and 8.2 million deaths worldwide. Lung cancer and breast cancer ranked first in the incidence and death of malignant tumors in men and women, respectively. The pathogenesis of cancer is still unclear, but it is closely related to population growth and aging, microbial infection, smoking, eating habits, family tumor history and environmental pollution. The incidence of malignant tumors in my country is generally on the rise, increasing at a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/158C12Q2600/166
Inventor 安汉祥张云周丹汤玮玮
Owner THE FIRST AFFILIATED HOSPITAL OF XIAMEN UNIV
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