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Anti-drug resistant infection polypeptide Cbf-14-2 and application thereof

An anti-drug-resistant bacteria, drug-resistant technology, applied in the direction of anti-bacterial drugs, peptides, pharmaceutical formulations, etc., can solve problems such as multi-drug resistant bacteria, and achieve the effect of low toxicity and low hemolytic activity

Active Publication Date: 2017-03-29
CHINA PHARM UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the widespread use and abuse of antibiotics has led to the emergence of multidrug-resistant bacteria, which has brought great challenges to clinical diagnosis and treatment.

Method used

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  • Anti-drug resistant infection polypeptide Cbf-14-2 and application thereof
  • Anti-drug resistant infection polypeptide Cbf-14-2 and application thereof
  • Anti-drug resistant infection polypeptide Cbf-14-2 and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0060] Preparation of polypeptide Cbf-14-2

[0061] Artificially synthesize the polypeptide of the present invention according to the conventional solid-phase synthesis method, and the prepared polypeptide sequence of Cbf-14-2 is:

[0062] Arginine-Leucine-Leucine-Arginine-Ornithine-Phenylalanine-Phenylalanine-Arginine-Ornithine-Leucine-Lysine-Lysine- Serine-Valine

[0063] Synthesis of peptides: The synthesis of peptides is carried out one by one from the C-terminal to the N-terminal. Soak Fmoc-Val-Wang Resin in dichloromethane for 15 minutes, wait for the resin to swell, and remove the dichloromethane; add hexahydropyridine / DMF solution with a volume ratio of 1:4, use nitrogen agitation, and react twice for 5 minutes and 15min, after the reaction, the resin was washed 9 times with DMF. Take a small amount of resin and add 2-3 drops of each color test agent ABC (A solution: ninhydrin / dehydrated ethanol solution; B solution: pyridine; C solution: phenol / dehydrated ethanol s...

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Abstract

The invention relates to the technical field of polypeptide drugs in biochemistry, in particular to a polypeptide and application thereof. An unnatural amino acid is introduced according to an antimicrobial peptide Cbf-14 to obtain the polypeptide Cbf-14-2 having the high inhibiting activity on drug-resistant bacteria. An amino acid sequence of the antibacterial polypeptide is shown as SEQ ID: NO:1. An in-vitro antibacterial activity research shows that the polypeptide Cbf-14-2 has a more remarkable killing effect on clinic drug-resistant bacteria compared with a parent peptide Cbf-14. An in-vivo bacteremia model research shows that the polypeptide Cbf-14-2 can remarkably improve the survival rate of a rat suffering from bacteremia, the weight of the rat suffering from the bacteremia is protected, the bacterium concentration of the polypeptide in blood is reduced, and meanwhile the polypeptide has the very good treating effect on lung tissue infection inflammation caused by drug-resistant strains. The polypeptide Cbf-14-2 has very small toxicity to mammalian cells.

Description

technical field [0001] The invention relates to the technical field of polypeptide drugs in biochemistry, in particular to a polypeptide and its application, that is, the application of the polypeptide to resist drug-resistant bacterial infection. Background technique [0002] Since the discovery of penicillin, antibiotics have been considered to have miraculous effects in anti-infection and have significantly improved the quality of human life. However, the widespread use and abuse of antibiotics has led to the emergence of multidrug-resistant bacteria, which has brought great challenges to clinical diagnosis and treatment. [0003] Antimicrobial peptides are a key component of the body's innate immune system and are ubiquitous in nature. Naturally occurring antimicrobial peptides are usually shorter amino acid sequences, between 15 and 40 in length. The surface of the antimicrobial peptide is positively charged, usually provided by arginine, lysine and histidine in natur...

Claims

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Application Information

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IPC IPC(8): C07K7/08A61K38/10A61P31/04
CPCA61K38/00C07K7/08Y02A50/30
Inventor 周长林刘含含魏杉杉康玮窦洁贾源宾
Owner CHINA PHARM UNIV
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