Micro-channel PCR amplification system based on electromagnetic propulsion

Abstract

The invention discloses a micro-channel PCR amplification system based on electromagnetic propulsion, and belongs to the field of biology, analytical chemistry and medical test. The system comprises an annealing low-temperature region, an extending moderate-temperature region, a thermal-change high-temperature region, a PCR reagent and an electromagnetic propulsion module. The micro-channel PCR amplification system based on electromagnetic propulsion is mainly used for PCR amplification operation applied to the inside of a space range under the condition of weight loss and follow-up construction of a real-time fluorescent PCR detection system with a miniature structure; the purpose of integrating a micro-channel circulating PCR amplification module and an electromagnetic propulsion module is achieved; a portable miniaturized PCR fluorescent real-time detection system which meets requirements of space operation is realized; and purposes of function integration, micro-structure, light weight, small size and full-automatic detection are achieved.

Description

technical field [0001] The invention relates to a microchannel PCR amplification system, which belongs to the fields of biology, analytical chemistry and medical detection, and in particular to a microchannel PCR amplification system based on electromagnetic propulsion. Background technique [0002] With the maturity of my country's manned spaceflight technology and the gradual extension of the space mission cycle, the requirements for astronauts' health protection will become higher and higher. The unmonitored microorganisms in the spacecraft seriously threaten the health of astronauts. During long-term spaceflight, the growth of microbial flora and the variation of strains will affect the performance of instruments and equipment and the working environment conditions, and cause harm to the normal operation of spacecraft and its instruments and equipment. In order to prevent the occurrence, spread and effective treatment of infectious diseases in space flight, the microorg...

AI Technical Summary

Problems solved by technology

At present, there are three ways to control the injection of single-channel PCR amplification reaction devices: 1. Applying the control operation of the stepping motor to the single-chip microcomputer to realize the movement of the PCR reagent in the three temperature zones, but the AD chip and the DA chip in the control circuit There is its own delay error. Since the PCR amplification reaction is a repeated process of 40 cycles, there are some inevitable errors in each cycle, which seriously affects the amplification results after 40 repeated cycles. For micro The error loss caused by the system structure is more obvious

Moreover, if the objective error is reduced while improving the quality of the circuit control chip, the cost of the equipment is increased and the effect is not obvious

In addition, due to the size limitation of the circuit itself, the integration of the system is reduced, and peripherals cannot be added to the detection and acquisition system in the future.

2. Using the principle of thermal expansion and contraction of gas, use a single-chip microcomputer to drive semiconductor refrigeration chips to change the pressure in the microchannel to push the reagents. This control method is nonlinear, and the control is relatively difficult, and the device requires a large space, which is inconvenient. Microscale; 3. Utilize the deformation of piezoelectric ceramics to form a squeezing force on the reagent in the microchannel, and then realize the push of the reagent in the microchannel. The disadvantage of this method is that the deformation is small and the driving force formed is limited. Under the current device conditions Insufficient for reagent movement in three temperature zones

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