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Multicomponent three-dimensional organism printing device and method based on multi-channel nozzle

A technology of three-dimensional biology and printing devices, which is applied in medical science, prosthesis, additive processing, etc., can solve problems such as limited application, limited cell survival rate, high solution viscosity, and high surface tension, and achieves broad application prospects. The effect of simple preparation method

Inactive Publication Date: 2017-03-22
RENMIN UNIVERSITY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

3D printing based on inkjet mode can usually mix a variety of cells, but the requirements for the viscosity and surface tension of the solution are high, and the application is severely limited
However, the 3D bioprinting technology based on ultraviolet light curing has certain limitations on the survival rate of cells because ultraviolet light has certain lethality on cells.

Method used

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  • Multicomponent three-dimensional organism printing device and method based on multi-channel nozzle
  • Multicomponent three-dimensional organism printing device and method based on multi-channel nozzle
  • Multicomponent three-dimensional organism printing device and method based on multi-channel nozzle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] like figure 2 Shown is a schematic diagram of the device of the three-channel nozzle provided in Example 1 of the present invention. In this embodiment, three glass capillaries are arranged side by side to form a multi-channel nozzle 1 , and the inner diameters of the glass capillaries are all 500 μm. Each glass capillary top uses Teflon tube 13 and liquid reservoir 3 ( figure 2 (not shown in ) and sealed with paraffin 14. The No. 1 ink that the present embodiment selects is the sodium alginate solution that the mass fraction is 5%; No. 2 and No. 3 inks are the calcium chloride (CaCl 2 ) aqueous solution.

[0041] After connecting the liquid channels respectively, the multi-channel nozzle 1 is fixed on the three-axis stepping motor 2, and the z-axis height is adjusted so that the distance between the multi-channel nozzle 1 and the printing substrate is less than 1mm. Start the printing program, set the printing speed to 20mm / s, and the liquid injection speed to 0....

Embodiment 2

[0043] like image 3 As shown, changing the No. 3 ink in Example 1 to ordinary blue ink, and printing with other parameters unchanged, a piece of blue gel can be printed, similarly, a variety of different gels can be mixed at different positions Ink, print out colored gel.

Embodiment 3

[0045] like Figure 4 As shown, the No. 3 ink in Example 1 was replaced with rat aortic endothelial cell suspension, and printing was performed with the same parameters, and a gel evenly loaded with cells could be obtained.

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Abstract

The invention relates to a multicomponent three-dimensional organism printing device and method based on multi-channel nozzle. The device comprises a multi-channel nozzle which is located on a three axis stepper motor. The multi-channel mozzle is made of multiple nozzles arranged in parallel. One end of all micro-channels is aligned to form the nozzle, and the other end is connected with all liquid storage containers through Teflon pipes. All liquid drive devices are connected with all the liquid storage containers to drive the liquid in the liquid storage containers. A terminal control system controls the three axis stepper motor, all the liquid drive devices and temperature control devices to make the multi-channel nozzle move correspondingly and eject ink, thus achieving the three dimensional printing on a temperature control substrate. The device can precisely adjust the proportion of different ink types through controlling the ink feeding speed of all the channels. The printed artificial tissues and cells are more close to the actual tissues or organs, thus making the device have a broader application prospect compared with the prior art of tissue engineering.

Description

technical field [0001] The present invention relates to a bioprinting device and method, in particular to a multi-component three-dimensional bioprinting device and method based on multi-channel nozzles. Background technique [0002] Since the concept of tissue engineering was proposed in the 1980s, its related development has greatly promoted the progress of modern biomedicine. Among them, the development of tissue engineering relies heavily on the construction of tissue scaffolds; however, traditional tissue scaffolds are limited by the existing material molding technology and can only obtain some simple structures. With the rapid development of 3D printing technology, many researchers have used 3D printing technology to assist tissue engineering and further applied it to the field of biomedicine, making significant progress. The cells are placed in the tissue scaffold in situ during the printing process, and after being formed, they are cultured in a certain culture envi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/02B33Y30/00B33Y10/00
CPCA61F2/02A61F2240/001B33Y10/00B33Y30/00
Inventor 王亚培廖声茏
Owner RENMIN UNIVERSITY OF CHINA
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