Technique for improving paraffin embedding of cell specimen

Abstract

The invention provides a technique for improving paraffin embedding of a cell specimen. The technique is characterized by comprising the following steps: depositing the cell specimen, fixing, performing centrifugal treatment, extracting the cell specimen, performing conventional fixation, dehydrating, performing transparency treatment, performing paraffin soaking treatment, performing paraffin embedding, slicing, dyeing, and packaging. The technique provided by the invention has the advantages of being convenient and rapid to operate, free of pollution, good in practical effect and very applicable to popularization and use in large hospitals as specimen treatment and dyeing processes can be implemented simultaneously with conventional pathological specimens, and not only is conventional inspection implemented, but also immunohistochemistry and special dyeing can be carried out.

Description

technical field [0001] The invention belongs to the cytological detection method in the fields of clinical pathological diagnosis and scientific research, and specifically relates to an improved technique for paraffin embedding of cell samples. Background technique [0002] Exfoliative cytology examination is one of the effective methods for tumor diagnosis. The small amount of hydrothorax and ascites absorbed during the preparation of ordinary smears cannot represent the status of the entire hydrothorax and ascites. Examine most of the cells in the pleural and ascites in order to observe the morphology of most cells, and the cell wax block is easy to preserve, which greatly improves the sensitivity and specificity of cytopathological diagnosis, and makes up for the limited and insufficient production of conventional cell smears. The inadequacies of multiple stains warrant further research and prospective studies. However, 10% neutral formalin is often used for pre-fixation...

AI Technical Summary

Problems solved by technology

However, 10% neutral formalin is often used for pre-fixation in the production of cell wax blocks. The fixation time is long, the cells in the cell wax blocks are scattered, not easy to form a block, and the cells are obviously lost; and the smell is strong, which is easy to cause serious health problems to the operator. damage