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Prostatic cancer diagnosis product and application thereof

A technology for prostate cancer and products, applied in the biological field, can solve the problems of misdiagnosed conditions, not very stable diagnosis schemes, and misdiagnosed diseases.

Inactive Publication Date: 2017-03-15
邱宾涛
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under normal circumstances, the PSA in the blood is not high (not higher than 4ng / ml). When in the state of prostate cancer and other prostate diseases, the increase of PSA has become the most sensitive tumor marker for screening prostate cancer, but the increase of PSA is also It is often related to non-prostate cancer diseases, such as prostatitis, benign prostatic hyperplasia and other urinary system diseases, so it is not easy to diagnose, and may even lead to misdiagnosis, misdiagnosis or delay of disease
In addition, when prostate cancer is diagnosed by an increase in PSA, the patient is often already in the middle and late stages, and the purpose of early diagnosis cannot be achieved.
However, the detection rate of needle biopsy is closely related to the number and location of biopsy, which is not a very stable diagnostic solution.

Method used

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  • Prostatic cancer diagnosis product and application thereof
  • Prostatic cancer diagnosis product and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 High-throughput sequencing screening of differentially expressed genes

[0038] 1. Sampling

[0039] Select 10 cases of fresh prostate cancer tissues and corresponding paracancerous tissues, samples from prostate cancer patients who underwent radical prostatectomy and bilateral pelvic lymph node dissection from October 2012 to December 2015 in Peking Union Medical College Hospital. Under the guidance of the pathologist, the prostate cancer and paracancerous tissues were immediately taken and put into liquid nitrogen, and stored in a -80°C low-temperature refrigerator after serial numbering.

[0040] 2. Total RNA extraction from tissue samples

[0041] use Reagent (invitrogen, Cat. No. 15596-018) was used to extract sample RNA, and the experimental operation was carried out according to the product manual. The specific operation was as follows:

[0042] Collect the samples and freeze them in liquid nitrogen. After taking them out, put the tissue samples in...

Embodiment 2

[0053] Example 2 RT-PCR verification of expression of LOC730668 in prostate cancer tissues and corresponding paracancerous tissues

[0054] 1. Materials

[0055] 15 prostate cancer patients were selected, and the samples were collected from prostate cancer patients who underwent radical prostatectomy and bilateral pelvic lymph node dissection from October 2012 to December 2015 in Peking Union Medical College Hospital. Immediately take prostate cancer and paracancerous tissues and put them into liquid nitrogen, and store them in a -80°C low-temperature refrigerator after serial numbering.

[0056] 2. Method

[0057] 2.1 Extract the total RNA from the tissue samples of the subjects, the same as the extraction method in Example 1.

[0058] 2.2 Synthesis of cDNA by reverse transcription

[0059] use III Reverse Transcriptase (invitrogen, Cat. No. 18080-044) was used for cDNA reverse transcription, and the experimental operation was carried out according to the product manual...

Embodiment 3

[0078] Example 3 Application of Gene Chip Diagnosis of Prostate Cancer

[0079] 1, the acquisition of material, with embodiment 2.

[0080] 2, the extraction of total RNA, with the method for embodiment 1.

[0081] 3. Gene chip verification

[0082] After the total RNA was linearized and amplified, the cy3-UTP labeled and fluorescently labeled cRNAs were purified using the RNEASY Mini Kit, and the labeled cRNAs were fragmented with Amhion’s RNA Fragmentation Reagents. Human whole gene expression profile chips (4x 44K genes) from Agilent Company of the United States were used, hybridized in a chip hybridization oven at 65°C for 17 hours, then eluted, stained, and finally scanned with an Agilent DNA MicroarrayScanner scanner.

[0083] After the hybridized chip reads the data points by the chip scanner, the data is imported into the analysis software, and the genes whose absolute value of the natural logarithm of the ratio of the two groups is greater than 2.0 or less than 0.5 ...

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Abstract

The invention discloses a prostatic cancer diagnosis product and application thereof. The product can diagnose a prostatic cancer by detecting the expression level of LOC730668 in serum, urine and tissue specimens of a subject. The invention further discloses application of the product in prostatic cancer high risk group screening, diagnosing, treating, monitoring and prognosing. By utilizing the product to diagnose the prostatic cancer, not only can early detection be quickly and effectively achieved, but also a treatment target and an important basis are provided for clinical application such as gene therapy, drug therapy and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a product for diagnosing prostate cancer and its application. Background technique [0002] Prostate cancer is a malignant tumor that occurs in the prostate tissue of men. It has become the second most common tumor in men worldwide and ranks sixth in cancer-related mortality. In developed countries and regions such as Europe and the United States, it is the most common malignant tumor in men, and its mortality rate ranks second among various cancers; in Asia, its incidence rate is lower than that of Western countries, but it has shown a rapid upward trend in recent years faster than developed countries in Europe and America. The incidence of prostate cancer in my country is also increasing year by year, ranking third among male genitourinary system malignancies, second only to bladder cancer and kidney cancer. Prostate cancer generally occurs after the age of 50, and 95% of it occu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/118C12Q2600/158
Inventor 邱宾涛吴志宏叶伟亮
Owner 邱宾涛
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