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Exposing method for transformation-mode nanomaterial induced mammalian cell malignant transformation

A nanomaterial, mammalian technology, applied in animal cells, vertebrate cells, embryonic cells, etc., can solve problems such as inability to accurately reflect real toxicity, and achieve the effect of being suitable for large-scale promotion, simple method and reliable results

Inactive Publication Date: 2017-03-15
ANHUI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The physical and chemical properties and genotoxicity of nanomaterials can be strongly affected by natural environmental conditions, resulting in the toxicity of "original state" nanomaterials not accurately reflecting the true toxicity of transformed nanomaterials in the environment; therefore, it is necessary to design a An effective exposure method for malignant transformation of cells induced by nanomaterials

Method used

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  • Exposing method for transformation-mode nanomaterial induced mammalian cell malignant transformation
  • Exposing method for transformation-mode nanomaterial induced mammalian cell malignant transformation

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Experimental program
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Effect test

Embodiment 1

[0031] The present invention provides a kind of technical scheme step as follows:

[0032] Step 1: culture normal mouse embryonic fibroblasts and mouse embryonic fibroblasts containing activating mutations in DMEM medium containing fetal bovine serum in an incubator;

[0033] Step 2: Take three dishes of normal mouse embryonic fibroblasts and mouse embryonic fibroblasts containing activating mutations in the logarithmic growth phase and 80% fullness after the treatment in step 1 and divide them into three groups. The group included a dish of normal mouse embryonic fibroblasts and a dish of mouse embryonic fibroblasts containing activating mutations, and the groups were marked, and the number of plants in each dish was 1×10 5 , the size of the culture dish is 60mm, and each group of cells is digested with trypsin;

[0034] Step 3: After the three groups of cells in step 2 grow to the logarithmic growth phase, discard the culture medium in step 2, add 4 ml of DMEM medium contai...

Embodiment 2

[0047] Proceed as follows:

[0048] Step 1: culture normal mouse embryonic fibroblasts and mouse embryonic fibroblasts containing activating mutations in DMEM medium containing fetal bovine serum in an incubator;

[0049] Step 2: Take three dishes of normal mouse embryonic fibroblasts and mouse embryonic fibroblasts containing activating mutations in the logarithmic growth phase and 80% fullness after the treatment in step 1 and divide them into three groups. The group included a dish of normal mouse embryonic fibroblasts and a dish of mouse embryonic fibroblasts containing activating mutations, and the groups were marked, and the number of plants in each dish was 1×10 5 , the size of the culture dish is 60mm, and each group of cells is digested with trypsin;

[0050] Step 3: After the three groups of cells in step 2 grow to the logarithmic growth phase, discard the culture medium in step 2, add 4 ml of DMEM medium containing 10% volume of fetal bovine serum to the first grou...

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Abstract

The invention discloses an exposing method for transformation-mode nanomaterial induced mammalian cell malignant transformation; normal mouse embryo fibroblasts and mouse embryo fibroblasts with activating mutation are cultured respectively in a culture medium, at least three vessels of cells are collected and divided into at least three groups, each group includes one vessel of normal mouse embryo fibroblasts and one vessel of mouse embryo fibroblasts with activating mutation, and the groups are marked; after the cells grow to logarithmic phase, supernate is removed, DMEM (Dulbecco's modified eagle medium) with fetal calf serum is added to the first group, DMEM with fresh liquid and fetal calf serum is added to the second group, and DMEM with 'transformation-mode' nanomaterial suspension and fetal calf serum is added to the third group; after conditioned media are removed, each vessel of cells in each group is rinsed; cyclic culture is carried out. The method has the advantages of good simplicity, low operation difficulty, low cost, good results reliability and the like, and is suitable for large-scale popularization.

Description

technical field [0001] The invention relates to the field of biological safety of nanomaterials, in particular to an exposure method for malignant transformation of mammalian cells induced by transformed nanomaterials. Background technique [0002] Nanomaterials have become a new type of environmental pollutants that humans are widely exposed to. It has been proven that they can cause DNA damage and gene mutation, but their carcinogenicity / acceleration is still inconclusive. Why can nanomaterials cause mutations but no carcinogenicity? It may be due to the current research ideas and research methods are limited. At present, most studies on the toxic effects and carcinogenicity of nanomaterials are focused on the "pristine" nanomaterials that have not entered the environment after synthesis. The association of carcinogenicity often lacks the necessary considerations, which means that the current nanosafety assessment is not comprehensive. Studies in recent years have shown...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073
CPCC12N5/0603
Inventor 王妹梅杜华汪思应刘亚坤曹瑞
Owner ANHUI MEDICAL UNIV
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