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Culture medium for promoting rapid germination of bacillus stearothermophilus spores

A thermophilic fat, culture medium technology, applied in the directions of bacteria, microorganism-based methods, microorganism determination/inspection, etc., can solve the problems of low spore germination rate, long time, etc., and achieve high germination rate, low price, and shortened time. Effect

Inactive Publication Date: 2017-03-15
SHINVA MEDICAL INSTR CO LTD
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  • Summary
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] When carrying out disinfection and sterilization monitoring, it is necessary to restore the culture of spores, induce the spores to germinate and grow to the vegetative stage, and then monitor the sterilization effect, but the germination rate of spores induced by the spore recovery medium in the ordinary sense is relatively low , and the time is long, therefore, it is necessary to research and improve the existing medium to quickly and effectively increase the germination rate of spores

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The components and contents of the medium for promoting rapid germination of Bacillus stearothermophilus spores are as follows: glucose 3g / L, peptone 6g / L, dipotassium hydrogen phosphate 1g / L, soluble starch 1.5g / L, alanine 0.3g / L L, leucine 0.5g / L, creatinine 0.1g / L, solvent is water.

[0017] Stir the above proportioned components at room temperature until completely dissolved, adjust the pH value to 7.0-7.2, and sterilize at 115°C for 30 minutes before use.

Embodiment 2

[0019] The components and content of the medium for promoting rapid germination of Bacillus stearothermophilus spores are as follows: glucose 4g / L, peptone 7g / L, dipotassium hydrogen phosphate 2g / L, soluble starch 2g / L, alanine 0.4g / L , leucine 0.6g / L, creatinine 0.2g / L, solvent is water.

[0020] Stir the above proportioned components at room temperature until completely dissolved, adjust the pH value to 7.0-7.2, and sterilize at 115°C for 30 minutes before use.

Embodiment 3

[0022] The components and content of the medium for promoting rapid germination of Bacillus stearothermophilus spores are as follows: glucose 5g / L, peptone 8g / L, dipotassium hydrogen phosphate 3g / L, soluble starch 2.5g / L, alanine 0.5g / L L, leucine 0.8g / L, creatinine 0.3g / L, solvent is water.

[0023] Stir the above proportioned components at room temperature until completely dissolved, adjust the pH value to 7.0-7.2, and sterilize at 115°C for 30 minutes before use.

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Abstract

The invention relates to a culture medium for promoting rapid germination of bacillus stearothermophilus spores, and belongs to the technical field of microorganism culture media. The culture medium comprises 3-5g / L of glucose, 6-8g / L of peptone, 1-3g / L of dipotassium phosphate, 1.5-2.5g / L of soluble starch, 0.3-0.5g / L of alanine, 0.5-0.8g / L of leucine and 0.1-0.3g / L of creatinine, wherein water serves as a solvent. The culture medium has the advantages that through addition of special inducing factors, the germination rate of the bacillus stearothermophilus spores is increased effectively, and time for the bacillus stearothermophilus spores recovering to propagules is shortened; the components of the culture medium are low in price and easy to get.

Description

technical field [0001] The invention relates to a culture medium for promoting rapid germination of bacillus stearothermophilus spores, belonging to the technical field of microbial culture medium. Background technique [0002] Bacillus stearothermophilus is an aerobic or facultative anaerobic bacterium, which belongs to Gram-positive bacteria. The bacterial spores are colored malachite green. It uses glucose to grow, produces acid but not gas, and its optimum growth and reproduction temperature is 55-60°C , the highest growth temperature is 65-75 ° C, which belongs to thermophilic bacteria, and the collection number of China Industrial Microorganism Culture Collection Center (CICC) is: 10267, that is, ATCC: 7953. [0003] The spores produced by Bacillus stearothermophilus are non-toxic and non-pathogenic, and have strong resistance to heat, radiation and chemical substances, especially to pressure steam and hydrogen peroxide, which are stronger than most microorganisms. The...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/04C12R1/07
CPCC12N1/20C12Q1/045
Inventor 赵健存韩宝福田友昊王庆州屈道银
Owner SHINVA MEDICAL INSTR CO LTD
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