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Method for preparing trigonelline from Abrus plants

A technology of trigonelline and Acacia genus, applied in the field of preparation of trigonelline, can solve the problems of complex extraction and separation methods, unsuitability for industrial production, and high solvent toxicity, and achieve short cycle, low solvent toxicity, and large preparation volume Effect

Inactive Publication Date: 2017-03-15
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no method for extracting and purifying trigonelline in Acacia guangzhou, Acacia mau and Acacia acacia, but the extraction and separation methods of trigonelline monomer components in other plants are complicated and the extraction rate is low. The solvent is highly toxic and not suitable for industrial production
[0007] Chinese patent application 201310706466.9 discloses a novel extraction method of wild trigonelline, which uses ultra-high pressure technology and macroporous resin adsorption and column chromatography coupling technology to enrich and purify trigonelline in fenugreek, and uses gradient ethanol to macroporous Adsorption resin is used for elution, and then acetone and petroleum ether in different proportions are used for gradient elution of silica gel column chromatography containing trigonelline semi-finished products. This method is complicated in process and the solvent used is more toxic

Method used

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  • Method for preparing trigonelline from Abrus plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Get 1000g of Acacia guangzhou, add 15 times of the weight of the medicinal material in purified water, ultrasonically extract 3 times, each time for 0.5 hours, filter, combine the filtrate, and concentrate the filtrate to obtain a water concentrate containing 1g of medicinal material per ml, and then Put the water concentrate on a 200-mesh neutral alumina column, elute it with 3 times the column volume of purified water until it is colorless, collect all the eluate that passes through the column, and concentrate the eluate to dryness under reduced pressure to obtain trigonelline crude purification;

[0027] (2) add its weight 10 times methanol to the crude purified trigonelline obtained in step (1) and extract 2 times, combine the extracts, filter with a 1000 molecular weight ultrafiltration membrane, concentrate the filtrate, and recrystallize to obtain high-purity trigonelline Trigonelline 3.5g.

Embodiment 2

[0029] (1) Get 1000g of Acacia magnolia, add 10 times the weight of the medicinal material in purified water, soak and extract 2 times, each time for 3 hours, filter, combine the filtrate, and concentrate the filtrate to obtain a water concentrate containing 0.2g of medicinal material per ml. Then put the water concentrate on a 100-mesh neutral alumina column, elute with purified water of 4 times the column volume until it is colorless, collect all the eluate that passes through the column, and concentrate the eluate to dryness under reduced pressure to obtain Fenugreek Alkaline crude purification;

[0030] (2) Add 15 times of its weight to the crude purified product of trigonelline obtained in step (1) to extract 2 times, combine the extracts, filter with 1000 molecular weight ultrafiltration membrane, concentrate the filtrate, and recrystallize to obtain high-purity trigonelline Trigonelline 3.2g.

Embodiment 3

[0032] (1) Get 1000g of acacia, add purified water 20 times the weight of the medicinal material, heat and reflux to extract 3 times, each time for 2 hours, filter, combine the filtrate, and concentrate the filtrate to obtain a water concentrate containing 0.1g of medicinal material per ml. Then put the water concentrate on a 200-mesh neutral alumina column, elute with purified water of 2 times the column volume until it is colorless, collect all the eluate that passes through the column, and concentrate the eluate to dryness under reduced pressure to obtain Fenugreek Alkaline crude purification;

[0033] (2) add its weight 15 times of ethanol to the crude purified product of trigonelline obtained in step (1) to extract 3 times, combine the extract, filter with 1000 molecular weight ultrafiltration membrane, concentrate the filtrate, and recrystallize to obtain high-purity trigonelline Trigonelline 3.8g.

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Abstract

The invention belongs to the field of natural drug extraction and relates to a method for preparing trigonelline from Abrus plants. The preparation method comprises that a Abrus plant such as Abrus cantoniensis Hance, Abrus mollis Hance or Abrus precatorius L. as a raw material is extracted by an aqueous solvent and is concentrated, the concentrated extract is separated and purified by column chromatography, the purified extract is subjected to ultrafiltration and the filtrate is subjected to recrystallization so that high purity trigonelline is obtained. The method has the advantages of high extraction purification yield, safety, stability, simple processes, low cost, high efficiency and short cycle, and can be used for industrial production.

Description

technical field [0001] The invention belongs to the field of natural medicine extraction, and in particular relates to a method for preparing trigonelline from plants of the genus Acacia. Background technique [0002] Aburs cantoniensis Hance, Abrus mollis Hance and Abrus precatorius L. are all leguminous plants of the genus Acacia, which have the functions of clearing heat and detoxifying, soothing the liver and relieving pain, etc., and are mostly used in the treatment of acute Diseases such as chronic hepatitis. [0003] Trigonelline, whose chemical name is N-methylnicotinic acid lactone or N-methylnicotinic acid inner salt, is soluble in water-soluble polar solvents such as water, methanol, ethanol, etc., and its molecular formula is C 7 h 7 NO 2 , the molecular weight is 137.14, and the structural formula is: [0004] [0005] Trigonelline exists in the whole plant of leguminous plant Acacia genus Aburs cantoniensis Hance, Abrus mollis Hance and Abrus precatorius...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D213/80C07D213/803
CPCC07D213/80C07D213/803
Inventor 袁旭江
Owner GUANGDONG PHARMA UNIV
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