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Non-toxic paulownia fortunei seedling culture method

A cultivation method, the technology of Paulownia albicans, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems affecting seedling quality, reproduction rate, rooting rate and survival rate, affecting seedling washing efficiency and transplanting survival rate, The detoxification time of tissue culture materials is long and other problems, so as to improve the efficiency of seedling washing and transplanting survival rate, high proliferation and rooting efficiency, and high rooting rate

Active Publication Date: 2017-03-15
GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the research on the industrialization of tissue culture and rapid propagation of Paulownia albacore clones, the applicant found that there are still three main problems affecting the industrial efficiency in the prior art: one is that the detoxification time of tissue culture materials is long, and the effect is not ideal , the detoxification efficiency is not high; the second is that after multiple subcultures on a single medium with high hormone concentration, the material is easy to vitrify, usually the vitrification rate of the fifth generation is about 20%, and with the number of subcultures Increasing the vitrification rate tends to increase, which greatly affects the seedling quality, reproduction rate, rooting rate and survival rate; the third is that when the tissue culture seedlings take root on the medium with agar as the support, a large amount of long ca. The 5mm fine hair absorbs a large amount of medium, and it is labor-intensive and time-consuming to wash the seedlings. It is easy to cause damage to the seedlings during washing, which affects the efficiency of seedling washing and the survival rate of transplanting.

Method used

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  • Non-toxic paulownia fortunei seedling culture method

Examples

Experimental program
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Effect test

Embodiment 1

[0032] 1) Using the stem segment of Paulownia albaflora (the Paulownia albaflora is an excellent clone of Paulownia albaflora obtained by screening according to the existing conventional screening method) with axillary buds from the Paulownia albaflora in the Guangxi Institute of Botany in northern Guangxi as an explant, place Soak in detergent solution with a mass concentration of 1% for 10 minutes, take it out and rinse it under running tap water, then place it on an ultra-clean workbench and soak it in ethanol with a volume concentration of 70% for 60 seconds, take it out and place it in a mass concentration 0.1% HgCl 2 Soak in water for 7 minutes, take it out, and wash it 5 times with sterile water;

[0033] 2) Inoculate the sterilized explants in the first-generation culture medium, cultivate them under light for 30 days, and develop and grow the axillary buds of the explants to form 2-3cm high sprouts (such as figure 1 shown), that is, the primary seedlings; wherein, th...

Embodiment 2

[0063] 1) Using the stem segment of Paulownia albaflora (the Paulownia albaflora is an excellent clone of Paulownia albaflora obtained by screening according to the existing conventional screening method) with axillary buds from the Paulownia albaflora in the Guangxi Institute of Botany in northern Guangxi as an explant, place Soak in detergent solution with a mass concentration of 1% for 10 minutes, take it out and rinse it under running tap water, then place it on an ultra-clean workbench and soak it in ethanol with a volume concentration of 70% for 50 seconds, take it out and place it in a mass concentration 0.1% HgCl 2 Soak in water for 6 minutes, take it out, and wash it 4 times with sterile water;

[0064] 2) Inoculate the sterilized explants in the first-generation culture medium, and cultivate them under light for 25 days. The axillary buds of the explants develop and grow, forming sprouts with a height of 2-3 cm, which are the first-generation seedlings. The resulting...

Embodiment 3

[0070] 1) Take the stem section with axillary buds of Paulownia albiflora (the Paulownia albiflora is an excellent clone C001 of Paulownia albiflora) as an explant, soak it in a detergent solution with a mass concentration of 1% for 10 minutes, take it out, and place it under flowing tap water Rinse it clean, then place it on the ultra-clean workbench and soak it in ethanol with a volume concentration of 75% for 70s, take it out and place it in HgCl with a mass concentration of 0.2% 2 Soak in water for 8 minutes, take it out, and wash 5 times with sterile water;

[0071] 2) Inoculate the sterilized explants in the first-generation culture medium, and cultivate them under light for 25 days. The axillary buds of the explants develop and grow, forming sprouts with a height of 2-3 cm, which are the first-generation seedlings. The resulting seedlings are strong, the leaves are bright green, and grow Good, induction rate 60%; wherein, the formula of the primary medium is: MS+6-BA 4....

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Abstract

The invention discloses a non-toxic paulownia fortunei seedling culture method which includes the steps: 1) taking paulownia fortunei stem segments with axillary buds as explants and performing primary culture after disinfection to form primary seedlings; 2) performing virus-free culture for the obtained seedlings to obtain virus-free materials; 3) alternately placing the obtained virus-free materials into a high-concentration plant hormone subculture medium and a low-concentration plant hormone subculture medium for light culture to obtain rank seedlings; 4) dividing the obtained rank seedlings and then performing rooting culture to obtain rooting seedlings. The method is simple to operate, high in virus-free, propagation and rooting efficiency and high in transplanting survival rate, the obtained rooting seedlings have developed root systems and short fine hair (<1mm), and labor and time are saved when the seedlings are cleaned.

Description

technical field [0001] The invention relates to plant tissue culture technology, in particular to a method for cultivating non-toxic Paulownia albaflower seedlings. Background technique [0002] Paulownia fortunei (Seem.) Hemsl is a tall tree of Scrophulariaceae and Paulownia genus, mainly produced in southern China, with a height of up to 30 meters, a straight trunk, and a diameter at breast height of up to 2 meters. Fast-growing and high-quality deciduous tree species. The characteristics of excellent clones of Paulownia alba are: (1) good stress resistance, strong adaptability, and can be planted in the south of the Yangtze River Basin; (2) fast growth, can be harvested in 5-6 years, average DBH 42cm, average mu Yield 33m 3 ; (3) Light material, good toughness, low density, high strength and bending strength, high economic value. [0003] In order to ensure that the traits of excellent trees can be inherited stably, their seedling propagation must be carried out by ase...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 黄宁珍苏江何金祥付传明冼康华黄惠锦龚庆芳
Owner GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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