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Competitive hog cholera virus antibody chemiluminescence quantitative detection kit

A technology for quantitative detection of swine fever virus, applied in chemiluminescence/bioluminescence, analysis through chemical reactions of materials, measurement devices, etc., can solve problems such as carcinogens, toxic substrates, and endogenous enzyme interference. It achieves the effects of low carcinogenicity and harm, short detection time and simplified operation process

Inactive Publication Date: 2017-03-08
CHINA INST OF VETERINARY DRUG CONTROL +1
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Problems solved by technology

Although these methods have high sensitivity and specificity, they have disadvantages such as endogenous enzyme interference, and most of the substrates are toxic or carcinogenic.

Method used

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  • Competitive hog cholera virus antibody chemiluminescence quantitative detection kit
  • Competitive hog cholera virus antibody chemiluminescence quantitative detection kit

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Embodiment Construction

[0026] A kit for quantitative detection of swine fever virus antibody competition chemiluminescence, including antigen-coated plates, enzyme-labeled antibodies, calibrator, concentrated washing solution, luminescent substrate A, and luminescent substrate B;

[0027] The antigen-coated plate uses the purified CSFV E2 protein as the coated antigen, and uses pH9.6 carbonate buffer solution to coat the CSFV E2 protein on an opaque polystyrene plate;

[0028] The enzyme-labeled antibody is a monoclonal antibody against classical swine fever virus labeled with horseradish peroxidase by an enzyme-labeled antibody; the glutaraldehyde method is used for labeling, that is, the monoclonal antibody to classical swine fever virus, glutaraldehyde and horseradish peroxide The enzyme is added to the container for cross-linking, and the resulting mixture is dialyzed to remove excess glutaraldehyde; an equal volume of glycerol is added to the dialyzed mixture, and stored at -20°C;

[0029] For ...

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Abstract

The invention relates to a competitive hog cholera virus antibody chemiluminescence quantitative detection kit, which comprises a coating antigen coated plate, an enzyme-labeled antibody, a calibrator, a washing concentrate, a luminescent substrate A and a luminescent substrate B, wherein a purified hog cholera virus E2 protein is taken as a coating antigen of the antigen coated plate; the enzyme-labeled antibody is a hog cholera virus monoclonal antibody labeled by horseradish peroxidase; hyperimmune serum immune to a live vaccine is diluted to concentrations of 0NU / mL, 2NU / mL, 5NU / mL, 10NU / mL, 30NU / mL and 60NU / mL by a calibrator diluent respectively; the luminescent substrate A contains luminol, hydroxycoumarin, gallic acid and a Tris-Hcl buffer solution. The kit has the advantages of short detection time, capability of greatly enhancing a detection signal, high sensitivity and the like.

Description

technical field [0001] The invention relates to a kit, in particular to a kit for quantitative detection of swine fever virus antibody competition chemiluminescence. Background technique [0002] Swine fever, also known as cholera, is commonly known as "rotten intestinal fever" in my country. It is a highly contagious infectious disease of pigs caused by swine fever virus. It will cause degeneration of small blood vessel walls, multiple hemorrhages, necrosis and infarction of internal organs. The disease spreads quickly, is popular, has high morbidity and mortality, and is extremely harmful. The World Health Organization lists it as a statutory reportable animal disease, and my country's 2008 revision of the "Records of Types I, II, and III Animal Diseases" lists it as a type I animal disease. The characteristics of swine fever in my country are atypical swine fever or mild swine fever and reproductive failure of pregnant sows as the main manifestations. Persistent infectio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N21/76
CPCG01N21/76G01N33/56983
Inventor 李秀梅王琴刘玉宝徐璐耿玉静任雪建李莹
Owner CHINA INST OF VETERINARY DRUG CONTROL
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