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A method of improving the resistance of bacterial strains to inhibitors by using micro-aeration

A technology of inhibitors and bacterial strains, applied in the field of bioengineering, can solve problems such as unfavorable biotransformation, and achieve the effects of increasing xylitol yield, increasing yield, and reducing residual xylose

Active Publication Date: 2019-11-05
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Lignocellulosic raw materials are mainly composed of cellulose, hemicellulose and lignin; cellulose and hemicellulose can be hydrolyzed to produce glucose, xylose, arabinose and other monosaccharides, but lignocellulosic raw materials must be pretreated After that, it can be hydrolyzed by biological enzymes; some toxic by-products generated during the pretreatment of lignocellulosic raw materials have an inhibitory effect on the subsequent fermentation process, which is not conducive to the progress of biotransformation;

Method used

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  • A method of improving the resistance of bacterial strains to inhibitors by using micro-aeration
  • A method of improving the resistance of bacterial strains to inhibitors by using micro-aeration
  • A method of improving the resistance of bacterial strains to inhibitors by using micro-aeration

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Embodiment 1

[0032] The micro-aeration of the present embodiment improves the method for the resistance of bacterial strains to cellulose hydrolyzate inhibitors, comprising the following steps:

[0033] 1) Take the Kluyveromyces marxianus 1727 (K.marxianus 1727) strain for seed cultivation and expanded cultivation; the cultivation process is specifically:

[0034] Seed culture: Prepare the seed liquid medium by sterilizing 10g / L of yeast powder, 20g / L of peptone, and 20g / L of glucose at 121°C for 15 minutes, and inoculate the K. marxianus 1727 strain to the culture medium, cultivated for 18h until the strain concentration reached OD 620 to 2~2.4;

[0035] Expansion cultivation: take the bacteria obtained in the seed cultivation, inoculate 6% of the inoculum into the shake flask containing the expansion medium, the expansion temperature is 30°C, the rotation speed is 150rpm, and the cultivation time is 14h until the strain concentration reaches OD 620 to 2~2.4;

[0036] The culture mediu...

Embodiment 2

[0041] In this embodiment, micro-aeration improves the resistance of bacterial strains to cellulose hydrolyzate inhibitors. The K. marxianus 1727 (K.marxianus 1727) bacterial strain is used for seed cultivation and expansion cultivation in the same manner as in Example 1. , and use the same fermentation broth and fermentation conditions for fermentation, the only difference is that the ventilation of 0.2vvm is maintained during the fermentation process;

Embodiment 3

[0043] In this embodiment, micro-aeration improves the resistance of bacterial strains to cellulose hydrolyzate inhibitors. The K. marxianus 1727 (K.marxianus 1727) bacterial strain is used for seed cultivation and expansion cultivation in the same manner as in Example 1. , and use the same fermentation broth and fermentation conditions for fermentation, the only difference is that the ventilation of 1vvm is maintained during the fermentation process;

[0044] Result: by figure 1 It can be seen that in the presence of compound inhibitors, when 100g / L glucose is used as the carbon source, the fermentation performance of aeration and no aeration is significantly different; under the condition of slight aeration, the glucose consumption rate and ethanol production rate are significantly accelerated; Fermentation was just over, but under the conditions of 0.2, 0.5, and 1vvm, the fermentation ended after 8h, 9h, and 9h respectively, and the time was shortened by 43%, 36%, and 36% r...

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Abstract

The invention provides a method for improving resistance of a bacterial strain to inhibitors through micro aeration. The micro aeration is conducted, so the resistance of the bacterial strain to the various inhibitors (formic acid, acetic acid, furfural and hydroxymethylfurfural) in a cellulosic hydrolysate is improved, and the fermentation performance of glucose and xylose are significantly improved. Xylose fermentation conducted by using the method is compared with a control group without ventilation, the xylose has resistance to the various inhibitors in the cellulosic hydrolysate, under the conditions of four kinds of mixed inhibitors with relatively high concentrations, the fermentation time is shortened, residual xylose is decreased greatly and the yield of xylitol is improved. The fermentation of glucose and xylose mixed carbon sources by using the method is compared with a control group without ventilation, under the conditions of four kinds of mixed inhibitors with relatively high concentrations, the method promotes the simultaneous fermentation of glucose and xylose, the yield of ethyl alcohol remains unchanged, and the yield of xylitol is improved.

Description

technical field [0001] The invention belongs to the field of bioengineering and relates to a method for improving the resistance of bacterial strains to inhibitors by using micro-aeration. Background technique [0002] Conversion of lignocellulosic feedstocks to biofuels, such as ethanol, can ease the need for gasoline and reduce CO by combining diverse natural grass cultivation, sustainable agriculture, and forestry 2 Moreover, the use of lignocellulosic ethanol as a vehicle fuel can reduce greenhouse gas emissions by 88% compared to gasoline, which is much higher than the 18% reduction of corn ethanol; [Yi-Kai Su. , Laura B. Willis., Thomas W. Jeffries., Effects of Aeration on Growth, Ethanol and Polyol Accumulation by Spathasporapassalidarum NRRL Y-27907 and Scheffersomyces stipitis NRRL Y-7124. Biotechnology and Bioengineering. 2015; 112:457–469 [0003] Lignocellulosic raw materials are mainly composed of cellulose, hemicellulose and lignin; cellulose and hemicellulose...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/06C12R1/645
CPCC12P7/06Y02E50/10
Inventor 袁文杰冯华良高教琪
Owner DALIAN UNIV OF TECH
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