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Preparation and application of carcino-embryonic antigen electrochemical immunosensor based on Au@Ag@Au marker

An immunosensor, carcinoembryonic antigen technology, applied in the field of electrochemical immunosensing, can solve the problems of high cost, large sample consumption, complicated operation, etc., and achieve the effect of promoting electron transfer, high sensitivity, and increasing sensitivity.

Inactive Publication Date: 2017-02-22
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the detection methods of carcinoembryonic antigen mainly include enzyme-linked immunoassay, high performance liquid chromatography, and microbial method. These methods have high sensitivity and selectivity, but the detection process requires expensive special instruments and complex pretreatment. Moreover, the operation is cumbersome and the sample consumption is large, so it is not suitable for rapid detection

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  • Preparation and application of carcino-embryonic antigen electrochemical immunosensor based on Au@Ag@Au marker
  • Preparation and application of carcino-embryonic antigen electrochemical immunosensor based on Au@Ag@Au marker

Examples

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Effect test

Embodiment 1

[0036] Example 1 Preparation method of carcinoembryonic antigen electrochemical immunosensor based on Au@Ag@Au labeling:

[0037] (1) with Al 2 o3 Polish the glassy carbon electrode (GCE) with a diameter of 4 mm, ultrasonically clean it in ethanol and ultrapure water for 3 min, and dry it with nitrogen; take 6 μL of the complex of nitrogen-doped graphene / carcinoembryonic antigen primary antibody (NG-Ab 1 ) dispersion solution onto the surface of the electrode, incubated in a refrigerator at 4 °C for 12 h, and washed with PBS (pH 7.4) buffer solution to obtain Ab 1 -NG / GCE;

[0038] (2) Take 10 μL of bovine serum albumin (BSA) solution with a mass fraction of 1% drop-coated on the surface of the electrode, incubate at 37°C for 1 h, block the non-specific binding site, and use PBS (pH 7.4) buffer solution Rinse the electrode surface to get BSA / Ab 1 -NG / GCE;

[0039] (3) Add 10 μL of a series of carcinoembryonic antigen standard solutions with a concentration of 0.01-100 ng / ...

Embodiment 2

[0041] Example 2 Preparation method of carcinoembryonic antigen electrochemical immunosensor based on Au@Ag@Au labeling:

[0042] (1) with Al 2 o 3 Polish a glassy carbon electrode (GCE) with a diameter of 4 mm, ultrasonically clean it in ethanol and ultrapure water for 3 min, and dry it with nitrogen; take 8 μL of the complex of nitrogen-doped graphene / carcinoembryonic antigen primary antibody (NG-Ab 1 ) dispersion solution onto the surface of the electrode, incubated in a refrigerator at 4 °C for 12 h, and washed with PBS (pH 7.4) buffer solution to obtain Ab 1 -NG / GCE;

[0043] (2) Take 10 μL of bovine serum albumin (BSA) solution with a mass fraction of 2% and drop-coat it on the surface of the electrode, incubate at 37°C for 1 h, block the non-specific binding site, and wash with PBS (pH 7.4) buffer solution Rinse the electrode surface to get BSA / Ab 1 -NG / GCE;

[0044] (3) Add 10 μL of a series of carcinoembryonic antigen standard solutions with a concentration of 0...

Embodiment 3

[0046] Example 3 A method for preparing a carcinoembryonic antigen electrochemical immunosensor based on Au@Ag@Au labeling:

[0047] (1) with Al 2 o 3 Polish the glassy carbon electrode (GCE) with a diameter of 4 mm, ultrasonically clean it in ethanol and ultrapure water for 3 min, and dry it with nitrogen; take 10 μL of the complex of nitrogen-doped graphene / carcinoembryonic antigen primary antibody (NG-Ab 1 ) dispersion solution onto the surface of the electrode, incubated in a refrigerator at 4 °C for 12 h, and washed with PBS (pH 7.4) buffer solution to obtain Ab 1 -NG / GCE;

[0048] (2) Take 10 μL of bovine serum albumin (BSA) solution with a mass fraction of 3% and drop-coat it on the surface of the electrode, incubate at 37°C for 1 h, block the non-specific binding site, and use PBS (pH 7.4) buffer solution Rinse the electrode surface to get BSA / Ab 1 -NG / GCE;

[0049] (3) Add 10 μL of a series of carcinoembryonic antigen standard solutions with a concentration of 0...

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Abstract

The invention relates to the technical field of electrochemical immunosense, in particular to preparation and application of a carcino-embryonic antigen electrochemical immunosensor based on an Au@Ag@Au core-shell nano-composite (Au@Ag@Au NPs) marker. Specifically, the surface of a glassy carbon electrode is modified by nitrogen-doped graphene (NG) to prepare an antibody capture substrate, Au@Ag@Au NPs tracks and marks a carcino-embryonic antigen second antibody, the second antibody marked by Au@Ag@Au NPs is captured on the surface of the sensor through sandwich immune response, and detection of a carcino-embryonic antigen is achieved by detecting electrochemical signals of hydrogen peroxide and hydroquinone in a PBS solution.

Description

technical field [0001] The invention relates to the technical field of electrochemical immunosensing, in particular to the preparation and application of a carcinoembryonic antigen electrochemical immunosensor based on gold-silver-gold core-shell nanocomposite (Au@Ag@Au NPs) labeling. Specifically, nitrogen-doped graphene (NG) was used to modify the surface of the glassy carbon electrode as the antibody capture substrate. Au@Ag@Au NPs tracked and labeled the second antibody of carcinoembryonic antigen. The secondary antibody was captured on the surface of the sensor, and the detection of carcinoembryonic antigen was realized by detecting the electrochemical signals of hydrogen peroxide and hydroquinone in PBS solution. Background technique [0002] Carcinoembryonic antigen (CEA) is an important tumor marker synthesized by the gastrointestinal tract and hepatopancreas derived from fetal endoderm, with a molecular weight of 150-300 kD. The mechanism of tumor detection is: the...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3278
Inventor 花小霞周长利夏方诠乔雪莹陈培培
Owner UNIV OF JINAN
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