Method for identifying Chinese agaricus sinodeliciosus

An identification method, a delicious technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of no quick method, complicated microscopic identification, low accuracy, etc., and achieve good application prospects and accurate identification High performance and accurate positioning effect

Inactive Publication Date: 2017-02-22
SHANGHAI ACAD OF AGRI SCI
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Problems solved by technology

However, microscopic identification is more complicated and the accuracy is not high
In the late 20th century, molecular sequences were gradually used in identification, but only as a reference
However, there has been no fast method for the identification of Agaricus species in China, especially for the identification of mushroom species with great economic value, which is convenient for safeguarding the rights and interests of researchers. Therefore, it is of great economic importance to discover and identify Agaricus species. It is imperative to identify and utilize meaning types

Method used

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  • Method for identifying Chinese agaricus sinodeliciosus
  • Method for identifying Chinese agaricus sinodeliciosus
  • Method for identifying Chinese agaricus sinodeliciosus

Examples

Experimental program
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Embodiment 1

[0038] Take wild Chinese delicious mushrooms from somewhere:

[0039] (1) External form identification

[0040] The external morphology of Chinese delicious mushroom (A. sinodeliciosus) includes: a large cap of 10-35cm, a thick stipe of 5-20cm, gills that change from pink and flesh color to brown to dark black, and a ring on the upper part of the stipe And the entire fruiting body is buried 45-55 centimeters underground with trees growing around it;

[0041] (2) Anatomical identification

[0042] Cut the Chinese delicious mushroom from the middle of the cap and the stipe, let it stand for 2 minutes, and the section changes from white to pink;

[0043] (3) Identification of chemical reagents

[0044] Take the pre-prepared 5% KOH solution, drop it on the surface of the cap of the fruiting bodies of fresh Chinese delicious mushrooms, let it stand for 5 minutes, and the titration point of the KOH solution turns light yellow;

[0045] (4) Molecular sequence identification

[0...

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Abstract

The invention relates to a method for identifying Chinese agaricus sinodeliciosus. The method includes the steps of (1) external form identifying; (2) microstructure identifying; (3) chemical reagent detection; (4) molecular sequence identifying. According to the method, the A. sinodeliciosus is identified with the method of combining identifying of the external form characteristic, changes in the color of an anatomical structure, a chemical reagent and the molecular sequence, and the method has the advantages of being high in identification accuracy and accurate in positioning; the method is representative in identification of Chinese agaricus sp. classes, and has good application prospects.

Description

technical field [0001] The invention belongs to the field of mushroom identification, in particular to an identification method of Chinese delicious mushroom (A. sinodeliciosus). Background technique [0002] The identification of mushrooms in my country has existed since the beginning of the 20th century, but it has always been relatively primitive, and the identification is not accurate, and in many cases it cannot be located at the species level. With the application of microscopy in mushroom identification, microstructure observation has been applied in the identification of mushrooms. However, microscopic identification is more complicated and the accuracy is not high. In the late 20th century, molecular sequences were gradually used in identification, but only as a reference. There are as many as thousands of species in the genus Agaricus, and the species in the genus Agaricus in China accounts for a large part of the world. However, there has been no fast method fo...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04
CPCC12Q1/6895C12Q1/04C12Q1/6869C12Q2531/113
Inventor 李传华曲明清李正鹏宋晓霞奚莉萍汪虹陈明杰李文张劲松鲍大鹏黄建春沈学香
Owner SHANGHAI ACAD OF AGRI SCI
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