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Method for preparing ethanol fermentation broth through fermentation of manganese alginate and lycoris radiate bulbs

A technology of Lycoris safflower and ethanol fermentation, which is applied in the field of fermentation and can solve the problems of low conversion rate of ethanol

Inactive Publication Date: 2017-02-22
周世容
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cost of ethanol is greatly reduced by fermenting ethanol based on Saccharomyces cerevisiae engineered bacteria, but the conversion rate of ethanol in the prior art is generally low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The method for preparing ethanol fermentation broth by fermenting manganese alginate and safflower bulbs of Lycoris radiata includes the following steps:

[0019] The first step is to soak the bulbs of Lycoris radiata in 45° white wine, soak them at room temperature for 6 days, take out the bulbs of Lycoris radiata, and use the soaked white wine for later use;

[0020] In the second step, mash the taken out bulb of Lycoris radiata, add α-amylase and cellulase, and cook for 50 minutes at 75°C;

[0021] In the third step, add manganese alginate to the liquor soaked in the first step according to the mass ratio of 9:1, heat it to 45°C, and add the safflower lycoris bulb after the second step of steaming;

[0022] The fourth step is to inoculate Saccharomyces cerevisiae while adding glucoamylase, the fermentation temperature is 35°C, the fermentation time is 25 hours, and the ethanol fermentation broth is obtained.

[0023] In the above process, based on 1 kg of Lycoris saffron bulbs...

Embodiment 2

[0025] The method for preparing ethanol fermentation broth by fermenting manganese alginate and safflower bulbs of Lycoris radiata includes the following steps:

[0026] The first step is to soak the bulbs of Lycoris safflower in 40° white wine, soak them for 5 days at room temperature, take out the bulbs of Lycoris safflower, and set aside the soaked white wine;

[0027] The second step is to mash the taken out safflower Lycoris bulbs, add α-amylase and cellulase, and cook for 40 minutes at 70°C;

[0028] In the third step, add manganese alginate to the liquor soaked in the first step according to the mass ratio of 9:1, heat it to 40°C, and add the safflower lycoris bulb after the second step of steaming;

[0029] The fourth step is to inoculate Saccharomyces cerevisiae while adding glucoamylase, the fermentation temperature is 30°C, and the fermentation time is 20 hours to obtain ethanol fermentation broth.

[0030] In the above process, based on 1 kg of Lycoris saffron bulbs, 4 g amy...

Embodiment 3

[0032] The method for preparing ethanol fermentation broth by fermenting manganese alginate and safflower bulbs of Lycoris radiata includes the following steps:

[0033] In the first step, soak the bulbs of Lycoris radiata in 50° white wine, soak them at room temperature for 7 days, take out the bulbs of Lycoris radiata, and set aside the soaked white wine;

[0034] The second step is to mash the taken out safflower Lycoris bulbs, add α-amylase and cellulase, and cook for 60 minutes at 80°C;

[0035] In the third step, add manganese alginate to the white wine soaked in the first step according to the mass ratio of 9:1, heat to 50°C, and add the safflower lycoris bulb after the second step steaming;

[0036] The fourth step is to inoculate Saccharomyces cerevisiae while adding glucoamylase, the fermentation temperature is 40°C, and the fermentation time is 30 hours to obtain ethanol fermentation broth.

[0037] In the above process, based on 1 kg of Lycoris saffron bulbs, 5 g amylase, 10...

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PUM

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Abstract

The invention discloses a method for preparing an ethanol fermentation broth through fermentation of manganese alginate and lycoris radiate bulbs. The method comprises steps as follows: step one, the lycoris radiate bulbs are soaked in 40%-50%(v / v) baijiu at the normal temperature for 5-7 days and then taken out, and the soaked baijiu is left for later use; step two, the lycoris radiate bulbs taken out in the step one are mashed, amylase and cellulose are added, and the mixture is cooked at the temperature of 70-80 DEG C for 40-60 min; step three, manganese alginate is added to the soaking baijiu in the step one in the mass ratio being 9:1, the mixture is heated to 40-50 DEG C, and the lycoris radiate bulbs cooked in the step two are added; step four, the mixture is inoculated with yeast for fermentation while saccharifying enzymes are added, and the ethanol fermentation broth is obtained. Glucose produced during saccharification can allow bacteria to grow and breed, fermentation and saccharification are performed simultaneously, glucose produced during saccharification is sufficiently utilized by the yeast once produced, the efficiency is improved, and microbiological contamination is prevented.

Description

Technical field [0001] The invention discloses a method for preparing ethanol fermentation broth by fermenting manganese alginate and safflower bulbs, which belongs to the field of fermentation. Background technique [0002] The bulbs of Safflower Lycoris contain many kinds of alkaloids, the main ones are high lycorine, lycorine, polydacticine, lycorine, lycorine, pseudolycorine and galanthinine. Alkaloids such as rain lycorine, nor-methyl lycorine, nor-methyl homolycorine, small star allinine, epigalanthine, streakine and cylindrossine, and contains two kinds of alkaloids that inhibit plant growth and resistance The carcinogenic components of Lycorisidine and Lycorisidine. [0003] The patent with the application number CN201410588693.0 discloses an engineered Saccharomyces cerevisiae using xylose for ethanol fermentation, and a preparation method and application. The strain contains the following nucleotide sequences: xylose reductase gene, xylitol dehydrogenase gene and xylul...

Claims

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Application Information

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IPC IPC(8): C12P7/06C12P19/14C12P19/02C12R1/865
CPCC12P7/06C12P19/02C12P19/14Y02E50/10
Inventor 周世容
Owner 周世容
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