A kind of grapevine extract and its application
A kind of technology of extracting and porcine, applied in the field of natural product chemistry
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Embodiment 1
[0017] Embodiment 1: the concentration of extraction solvent (ethanol) is selected
[0018] 1. Take 20 g of the dried and pulverized powder of the stems and leaves of grape vines, immerse them in 200 ml of pure water at room temperature and extract them ultrasonically twice, each time for 60 minutes (500 W), combine the filtrates after filtration, recover the solvent under reduced pressure to dryness, and obtain crude vines Extract; take the obtained grape pan crude extract and prepare a 0.05mg / ml solution for later use.
[0019] 2. Take 20 g of the powder after drying and pulverizing the stems and leaves of grape vines, immerse them in 200 ml of ethanol solution with a volume concentration of 30% at room temperature and extract them ultrasonically twice, each time for 60 min (500 W), filter and combine the filtrates, and recover the solvent under reduced pressure to dryness , to obtain the crude extract of grape pan; the obtained crude extract of grape pan was prepared into a...
Embodiment 2
[0034] Embodiment 2: the selection of solid-liquid ratio
[0035] 1. Take 20 g of the powder after drying and pulverizing the stems and leaves of grape vines, immerse them in 160 ml of ethanol solution with a volume concentration of 70% at room temperature, and extract them ultrasonically twice, each time for 60 min (500 W). After filtering, combine the filtrates, and recover the solvent under reduced pressure to dryness. , to obtain the crude extract of grape pan; the obtained crude extract of grape pan was prepared into a solution of 0.05 mg / ml for subsequent use.
[0036] 2. Get 20 g of the powder after drying and pulverizing the stems and leaves of grape vines, immerse them in 200 ml of ethanol solution with a volume concentration of 70% at room temperature, and extract them ultrasonically for 2 times, each time for 60 min (500 W). , to obtain the crude extract of grape pan; the obtained crude extract of grape pan was prepared into a solution of 0.05 mg / ml for subsequent u...
Embodiment 3
[0043] Embodiment 3: the preparation of grape pan extract
[0044] The preparation of the crude extract of the grape pan: get the powder 20g after the dry pulverization of the stem and leaf of the grape pan, soak it with 200ml of ethanol solution with a volume concentration of 70% at room temperature and extract it by ultrasonic for 2 times, each time for 60min (500W), filter and combine the filtrate, The solvent was recovered under reduced pressure to dryness, and the crude extract of grape pan was obtained, which was used for efficacy research.
[0045]Preparation of the active-enriched extract of Grapevine: Take 100 g of the dried and pulverized powder of Grapevine stems and leaves, soak it with 1000 ml of ethanol solution with a volume concentration of 70% at room temperature and extract it twice with ultrasound, each time for 60 minutes (500 W), filter and combine Filtrate (about 1800ml), recover the solvent under reduced pressure until there is no alcohol smell, pass thr...
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