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Method and kit for detecting human fructosediphosphate aldolase B gene

A bisphosphate aldolase and gene detection technology, which is applied in the field of genetic engineering, can solve the problems of requiring radiography, high technical requirements and low resolution, and achieves the effects of reducing false positives, simplifying the operation process and reducing the operation steps.

Active Publication Date: 2017-02-15
广东安科华南生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that the operation is complicated, the operator has high technical requirements, radiography is required, the resolution is not high, and it takes a long time

Method used

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  • Method and kit for detecting human fructosediphosphate aldolase B gene
  • Method and kit for detecting human fructosediphosphate aldolase B gene
  • Method and kit for detecting human fructosediphosphate aldolase B gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] ①Establishment of standard atlas

[0057] According to the above-mentioned use method, the control gene sequence is detected, and the following results are obtained: figure 1 with 2 The three-site detection map of the normal sequence compared with the wild-type specific primer and the three-site detection map of the mutant sequence with the mutant-type specific primer are shown;

[0058] ②Sample testing and comparison

[0059] Test No. 1 sample according to the above-mentioned method, and obtain the following results: image 3 As shown in the spectrum, it can be seen that sample No. 1 is a single point mutation at the A150P site.

Embodiment 2

[0061] Its overall flow process is identical with embodiment 1, and the testing result of No. 2 sample is as follows Figure 4 As shown, it can be seen that sample No. 2 has a single point mutation at the A175D site.

Embodiment 3

[0063] Its overall flow process is identical with embodiment 1, and the detection result of No. 3 sample is as follows Figure 5 As shown, it can be seen that sample No. 3 has a single point mutation at the N335K site.

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Abstract

The invention provides a method and a kit for detecting the human fructosediphosphate aldolase B gene. The detection kit comprises a primer pair for specifically amplifying three polymorphic sites on the human fructosediphosphate aldolase B gene, wherein the three polymorphic sites are respectively A150P, A175D and N335K, the primer pair comprises a common upstream primer and genotyping downstream primers, and the genotyping downstream primers comprise a wild type specific primer and a mutant type specific primer. With the adoption of the kit, the genotypes of the three polymorphic sites on the human fructosediphosphate aldolase B gene can be rapidly and efficiently detected, and the kit belongs to the kit for screening the human fructosediphosphate aldolase B gene, which is rapid, easy and convenient to operate, and is economical and efficient.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a human fructose diphosphate aldolase B gene detection kit and a detection method thereof. Background technique [0002] Hereditary Fructose Intolerance (HFI) is a kind of fructose metabolism disease. It is an autosomal recessive genetic disease. Deficiency or reduced activity, resulting in abnormal fructose metabolism in patients. The incidence rate in Europe is about 1:26100, while the incidence rate in my country is still unclear. With the improvement of people's living standards, the consumption of sugar is increasing, and the incidence of HFI will gradually increase. [0003] Clinically, children with hereditary fructose intolerance are rare. Since most formulas contain sucrose, newborns who are fed artificially after birth can experience vomiting, diarrhea, dehydration, and shock within 2-3 days and bleeding tendency and other symptoms of acute liver failure....

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/68C12Q1/6883C12Q1/686C12Q2600/156C12Q2563/107C12Q2545/101
Inventor 杜蔚安黄源坚郑文彦高静王邦超彭百华周玉吴智锋
Owner 广东安科华南生物科技有限公司
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