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Process for preparing fish skin antifreeze peptide through one-step enzymatic hydrolysis

An antifreeze peptide and enzymatic hydrolysis technology, which is applied in the field of one-step enzymatic hydrolysis to prepare fish skin antifreeze peptides, can solve the problems of frost cracking and water separation, and achieve the effect of less operation steps, low requirements for equipment and instruments, and low cost of consumables

Active Publication Date: 2017-02-15
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process of the present invention provides a low-cost, simple and fast method for preparing fish skin antifreeze peptides with a high yield of antifreeze peptides. The prepared fish skin antifreeze peptides are added to surimi products to solve the problem of surimi. The repeated freezing and thawing of products in the process of frozen storage, transportation and sales leads to key common technical problems such as cracking and water separation

Method used

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  • Process for preparing fish skin antifreeze peptide through one-step enzymatic hydrolysis
  • Process for preparing fish skin antifreeze peptide through one-step enzymatic hydrolysis
  • Process for preparing fish skin antifreeze peptide through one-step enzymatic hydrolysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Material selection: choose the skin of Spanish mackerel, which is more produced near the coast, as the raw material for antifreeze peptide extraction;

[0029] (2) Cleaning: Wash the inside and outside of the taken Spanish mackerel skin with water, control the water temperature at 4°C, and remove the excess debris adhered to the inside of the fish skin;

[0030] (3) Homogenization: Mix fish skin and water at a W / V ratio of 1:5, and place in a beater for homogenization;

[0031] (4) Enzymolysis: Alkaline protease was used to enzymolyze the fish skin homogenate, the ratio of enzyme to substrate was controlled to be 1:15, the pH of enzymolysis was 8, the temperature of enzymolysis was 40°C, and the time of enzymolysis was 90min. Cryopeptide crude solution;

[0032] (5) Membrane filtration: After centrifuging the crude solution of antifreeze peptide, take the supernatant, and select ultrafiltration membranes with molecular weight cut-offs of 20kDa, 10kDa and 5kDa under...

Embodiment 2

[0036] (1) Material selection: choose the skin of Spanish mackerel, which is more produced near the coast, as the raw material for antifreeze peptide extraction;

[0037] (2) Cleaning: Wash the inside and outside of the taken mackerel skin with water, control the water temperature at 7°C, and remove the excess debris adhering to the inside of the fish skin;

[0038] (3) Homogenization: Mix fish skin and water at a W / V ratio of 1:5, and place in a beater for homogenization;

[0039] (4) Enzymolysis: Alkaline protease was used to enzymolyze the fish skin homogenate, the ratio of enzyme to substrate was controlled at 1:20, the pH of enzymolysis was 8.5, the temperature of enzymolysis was 45°C, and the time of enzymolysis was 100min. Cryopeptide crude solution;

[0040] (5) Membrane filtration: After centrifuging the crude solution of antifreeze peptide, take the supernatant, and select ultrafiltration membranes with molecular weight cut-offs of 20kDa, 10kDa and 5kDa under the co...

Embodiment 3

[0044] (1) Material selection: choose the skin of Spanish mackerel, which is more produced near the coast, as the raw material for antifreeze peptide extraction;

[0045] (2) Cleaning: Wash the inside and outside of the taken mackerel skin with water, control the water temperature at 10°C, and remove the excess debris adhered to the inside of the fish skin;

[0046] (3) Homogenization: Mix fish skin and water at a W / V ratio of 1:5, and place in a beater for homogenization;

[0047] (4) Enzymolysis: Alkaline protease was used to enzymolyze the fish skin homogenate, the ratio of enzyme to substrate was controlled to be 1:30, the pH of enzymolysis was 9, the temperature of enzymolysis was 50°C, and the time of enzymolysis was 120min. Cryopeptide crude solution;

[0048] (5) Membrane filtration: After centrifuging the crude solution of antifreeze peptide, take the supernatant, and select ultrafiltration membranes with molecular weight cut-offs of 20kDa, 10kDa and 5kDa under the con...

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Abstract

The invention discloses a process for preparing a fish skin antifreeze peptide through one-step enzymatic hydrolysis. The fish skin antifreeze peptide is prepared by steps of selecting materials, cleaning the materials, homogenizing the materials, enzymatically hydrolyzing the materials, conducting membrane filtration, conducting double-effect vacuum concentration and conducting freeze-drying. The process provided by the invention is simple and low in production cost, and the process can reduce intermediate steps of preparing the antifreeze peptide, so that the loss of the antifreeze peptide caused by the intermediate steps is relieved and the yield of the antifreeze peptide is improved; and by controlling an enzymatic hydrolysis process, the obtained antifreeze peptide is relatively high in heat stagnation activity which is significantly higher than that of pig skin and chicken skin; and the industrial production of the antifreeze peptide is achieved.

Description

technical field [0001] The invention belongs to the technical field of aquatic product processing, and in particular relates to a process for preparing fish skin antifreeze peptide through one-step enzymatic hydrolysis. Background technique [0002] Mackerel (Scomberomorus niphonius) belongs to the order Perciformes, Mackerelidae, and the genus Mackerel. It is long and flat on the sides, with very small scales, and its shape resembles mackerel. It is widely distributed in my country's Yellow Sea, Bohai Sea, East China Sea, South China Sea and Taiwan Strait, with the highest yield in Yellow Sea and Bohai Sea. Fishery resources are quite rich, and it is one of the important marine economic fish in our country. And its skin is rich in collagen, which provides a good raw material for enzymatic hydrolysis to prepare antifreeze peptides. [0003] Antifreeze is a kind of substance that can lower the freezing point of the solution after being added to other solutions, thereby impr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/36C07K1/34C07K1/14
CPCC07K14/78C12P21/06
Inventor 张怡郑宝东傅维擎曾红亮郭泽镔
Owner FUJIAN AGRI & FORESTRY UNIV
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