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Preparation method and application of medicine-carrying nano-diamond compound

A nano-diamond and drug-loaded nanotechnology, which is applied in the field of tumor treatment, can solve the problems of single drug-loaded nano-diamond and poor curative effect, and achieve the effects of inhibiting growth and metastasis, prolonging life, and improving aggregation

Inactive Publication Date: 2017-02-15
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the shortcomings of the prior art described above, the purpose of the present invention is to provide a preparation method and application of a drug-loaded nano-diamond composite, which is used to solve the problems of single drug-loaded nano-diamond and poor curative effect in the prior art.

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  • Preparation method and application of medicine-carrying nano-diamond compound
  • Preparation method and application of medicine-carrying nano-diamond compound
  • Preparation method and application of medicine-carrying nano-diamond compound

Examples

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Embodiment 1

[0033] A preparation method of drug-loaded nano-diamond composites, comprising the following steps: 1) Take 10 mg of nano-diamonds (abbreviated as NDs), add 5 ml of deionized water, and use 50w ultrasonic dispersion for about 0.5 h at normal temperature and pressure to obtain shallow Brown clear and translucent solution, namely the first solution.

[0034] 2) Weigh 2 mg of doxorubicin hydrochloride, prepare a solution with 2 ml of deionized water, keep the reaction away from light, and ultrasonically assist the solution until the solution is clear and translucent to obtain the second solution.

[0035] 3) Add the second solution into the first solution under normal temperature and magnetic stirring to prepare the third solution.

[0036] 4) Take 0.02 mol / l NaOH solution and add it into the third solution to adjust the pH of the solution to 12, and react for 12 hours under normal temperature and magnetic stirring.

[0037] 5) After the reaction solution is centrifuged, the sup...

Embodiment 2

[0046] A preparation method of drug-loaded nano-diamond composites, comprising the following steps: 1) Take 10 mg of nano-diamonds (abbreviated as NDs), add 2 ml of deionized water, and use 100w ultrasonic dispersion for about 1.0 h at normal temperature and pressure to obtain shallow Brown clear and translucent solution, namely the first solution.

[0047] 2) Weigh 4 mg of doxorubicin hydrochloride, prepare a solution with 2 ml of deionized water, keep the reaction away from light, and ultrasonically assist the solution until the solution is clear and translucent to obtain the second solution.

[0048] 3) Add the second solution into the first solution under normal temperature and magnetic stirring to prepare the third solution.

[0049] 4) Add 0.02 mol / L NaOH solution to the third solution to adjust the pH of the solution to 13.0, and react for 12 hours under normal temperature and magnetic stirring.

[0050] 5) After the reaction solution was centrifuged, the supernatant was...

Embodiment 3

[0059] A preparation method of drug-loaded nano-diamond composite, comprising the steps of:

[0060] 1) Take 10mg of nano-diamonds (abbreviated as NDs), add 1ml of deionized water, and use 150w ultrasonic dispersion at normal temperature and pressure for about 1.5h to obtain a light brown clear and translucent solution, which is the first solution.

[0061] 2) Weigh 5 mg of doxorubicin hydrochloride, prepare a solution with 2 ml of deionized water, keep the reaction away from light, and ultrasonically assist the solution until the solution is clear and translucent to obtain the second solution.

[0062] 3) Add the second solution into the first solution under normal temperature and magnetic stirring to prepare the third solution.

[0063] 4) Add 0.02 mol / L NaOH aqueous solution to the third solution to adjust the pH of the solution to 13.6, and react for 12 hours under normal temperature and magnetic stirring.

[0064] 5) After the reaction solution is centrifuged, the supern...

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Abstract

The invention provides a preparation method and application of a medicine-carrying nano-diamond compound, wherein the method comprises the following steps: 1) mixing nano-diamond with water to prepare a first solution; 2) mixing doxorubicin hydrochloride with water to prepare a second solution; 3) mixing the first solution with the second solution to prepare a third solution; 4) adding a sodium hydroxide aqueous solution into the third solution to react completely; 5) centrifuging a reaction liquid, and collecting a sediment to prepare a doxorubicin-nano-diamond compound; 6) mixing the compound with water to prepare a fourth solution; 7) mixing the fourth solution with an all-transretinoic acid, then adding isopropanol or dimethylsulfoxide to react to prepare a fifth solution; and 8) centrifuging the fifth solution, and collecting a sediment to prepare the medicine-carrying nano-diamond compound. The two kinds of medicines are used in a combination manner, the growth and the metastasis of a tumor are inhibited, and the life of a living body is prolonged.

Description

technical field [0001] The invention relates to the field of tumor treatment, in particular to a preparation method and application of a drug-loaded nano-diamond compound. Background technique [0002] Nano-drug delivery systems have broad prospects for the treatment of malignant tumors, but there are problems in the course of treatment that lead to tumor recurrence and metastasis due to drug resistance of tumor cells. The latest research has found that drug-loaded nanodiamonds (NDs) can effectively overcome drug resistance and inhibit the growth and metastasis of tumor cells. The research published in "Science Translational Medicine" shows that tumor cells that can pump out anti-tumor drugs cannot transport nanodiamonds out of the cells, so it can help the drugs bound to nanodiamonds to stay in the cells. This conclusion is undoubtedly Overcoming drug resistance and increasing drug retention in tumor tissue provide new strategies. However, the clinical application of drug...

Claims

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Application Information

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IPC IPC(8): A61K9/14A61K47/04A61K31/704A61K9/08A61K9/00A61K41/00A61K47/12A61K47/10A61K47/20A61P35/00A61K31/203
CPCA61K31/704A61K9/0009A61K9/0019A61K9/08A61K9/143A61K31/203A61K41/0047A61K47/10A61K47/20A61K2300/00
Inventor 李桦楠曾德平刘宏英黄楠涂道月
Owner CHONGQING MEDICAL UNIVERSITY
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