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Application of indole-3-carbinol or dimer thereof

A technology of methanol and indole, applied in the field of application of indole-3-carbinol or its dimer, to achieve high safety effect

Inactive Publication Date: 2017-02-08
黄晶
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, there are no experimental and clinical research reports on the use of I3C for anti-atherosclerosis at home and abroad.

Method used

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  • Application of indole-3-carbinol or dimer thereof
  • Application of indole-3-carbinol or dimer thereof
  • Application of indole-3-carbinol or dimer thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1I3

[0047] Embodiment 1I3C is to the influence experiment of vascular smooth muscle cell

[0048] The proliferation of vascular smooth muscle cells is an important link in the development of atherosclerosis, and some clinical anti-atherosclerosis treatments are achieved by reducing the proliferation of vascular smooth muscle cells. Therefore, we conducted the following research on the effect of I3C on vascular smooth muscle cells, and the results are shown in Table 1-Table 5.

[0049] The experiment of the effect of I3C on the growth kinetics of vascular smooth muscle cells is that the VSMCs group with different concentrations of I3C in the medium is the test group, and the VSMCs without adding it is the control group. MTT colorimetric method was used to describe the growth of VSMCs treated with different concentrations of I3C for 120 hours; the effect of I3C on cell proliferation was observed by plate cloning experiment for 3 weeks; VSMCs proliferation and apoptosis, the results...

Embodiment 2

[0067] Example 2 Effect of DIM on vascular smooth muscle cells

[0068] The effect of DIM on the growth of VSMCs: the experiment was divided into 8 groups, the control group (no drug group): conventional culture; DMSO group: adding DMSO equal to the volume of the drug group in the medium; The final concentrations in the solution were 5, 25, 50, 100, 200, 400 μM, respectively. The growth of VSMCs treated with different concentrations of DIM for 3 days was described by MTT colorimetry. see results Figure 7 , Figure 8 , Figure 9 .

[0069] The results showed that each concentration of DIM had different inhibitory effects on the growth of VSMCs. Significant difference appeared after 24 hours of DIM50μM / l action, and after 72 hours of DIM action, there was also a significant difference between the 5μM / l group and the control group.

Embodiment 3I3

[0070] Example 3 Effects of I3C and DIM on VSMCs cell cycle

[0071] The experiment was divided into 6 groups, control group (no drug group): conventional culture; DMSO group, experimental group: add I3C and DIM to each group to make the final concentration in the culture solution 25umol / l, 50umol / l acted on VSMCs for 48 hours , flow cytometry analysis of its effect on the cell cycle. see results Figure 10 .

[0072] The results showed that DIM 50umol / l treated VSMCs for 48 hours, induced significant G1 phase arrest, and G0 / G1 phase cells increased significantly, which was significantly different from that of the control group. There was no significant difference between the cells in phase and the control group.

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PUM

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Abstract

The invention relates to the field of medicines and particularly relates to an application of indole-3-carbinol. The indole-3-carbinol is introduced to vascular aging preventing researches and is expected to delay progression of diseases, such as coronary heart disease, cerebral apoplexy and Alzheimer disease, related to vascular aging such as atherosclerosis, to prolong lifetime and to promote health of the aged. The indole-3-carbinol in a scheme is convenient to use and low in treatment cost, and lays a foundation for treatment of cardiovascular and cerebtovascular disease related to vascular aging such as atherosclerosis by utilizing the I3C and oligomer medicines thereof.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of indole-3-carbinol or its dimer. Background technique [0002] With the development of society and the improvement of living standards, the death caused by infectious diseases has been decreasing, while the death caused by arteriosclerosis has increased rapidly, and has become the main cause of global population death. Arteriosclerosis can be manifested as increased arterial wall hardness and atherosclerosis, and atherosclerosis is closely related to the aging of human blood vessels. Hard and narrow occlusion are the main pathological features. Atherosclerosis is a chronic inflammatory disease. Endothelial damage or high serum cholesterol levels lead to the deposition of a large number of cholesterol-based lipid particles under the arterial endothelium; these deposited lipids are then modified and marked to attract monocytes, lymphocytes, etc. in the blood to migrate t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/404A61P9/10A61P25/28A61P25/00A61P9/00A61P39/06
CPCA61K31/404
Inventor 黄晶江永红杨刚
Owner 黄晶
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