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Detection method of ochratoxin a in capsicum extract

A technology of chili extract and ochratoxin, which is applied in the field of detection of ochratoxin A in chili extract, can solve the problems of inapplicability, high detection cost, and long detection process, and achieve the goal of saving cost and improving detection efficiency Effect

Active Publication Date: 2019-03-19
CHENGUANG BIOTECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The above methods all use immunoaffinity columns, which have low detection efficiency, long detection process and high detection cost, and are not suitable for complex matrix products such as pepper extracts.

Method used

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  • Detection method of ochratoxin a in capsicum extract
  • Detection method of ochratoxin a in capsicum extract
  • Detection method of ochratoxin a in capsicum extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: The detection method of ochratoxin A in the capsicum extract adopts the following specific techniques.

[0018] (1) Preparation of sample solution: Weigh 1 g of capsicum extract, add 100% methanol solution 100 mL; sonicate in an ultrasonic cleaner for 10 min at an ultrasonic frequency of 40 kHz; then centrifuge at 10,000 r / min and 0°C for 5 min; take the supernatant 5mL into a 25mL volumetric flask, dilute to the mark with PBS solution, shake well; filter into a round bottom flask with glass microfiber filter paper, and then concentrate the filtrate to dryness by rotary evaporation, the vacuum of rotary evaporation is -0.08MPa, The temperature is 50°C; add 1mL of 50 vol% methanol and ultrasonically dissolve, pass through a 0.45μm filter membrane to obtain a sample solution.

[0019] Among them, the PBS solution configuration method is: 7.9g sodium chloride, 1.8g dipotassium hydrogen phosphate, 0.24g potassium dihydrogen phosphate, 0.2g potassium chloride, dis...

Embodiment 2

[0024] Example 2: The detection method of ochratoxin A in the capsicum extract adopts the following specific process.

[0025] (1) Preparation of sample solution: Weigh 1 g of capsicum extract, add 80 mL of 95vol% ethanol; sonicate in an ultrasonic cleaner for 20 min at an ultrasonic frequency of 60 kHz; then centrifuge at 8000 r / min at -5 °C for 7 min; take the supernatant 5mL into a 25mL volumetric flask, dilute to the mark with PBS solution, and shake well; filter the glass fiber filter paper into a round bottom flask, and concentrate the filtrate to dryness by rotary evaporation, the vacuum degree of rotary evaporation is -0.1MPa, and the temperature is 40 ℃; add 1mL, 50 vol% ethanol solution and ultrasonically dissolve, pass through a 0.45μm filter membrane to obtain a sample solution. Wherein, the PBS solution configuration method is the same as that in Example 1.

[0026] (2) Detection: 50 μL of sample solution was taken and detected by liquid chromatography-fluorescen...

Embodiment 3

[0030] Example 3: The detection method of ochratoxin A in the capsicum extract adopts the following specific process.

[0031] (1) Preparation of sample solution: Weigh 1 g of capsicum extract, add 20 mL of 95 vol% methanol; sonicate in an ultrasonic cleaner for 30 min at an ultrasonic frequency of 100 kHz; then centrifuge at 8000 r / min and -5 °C for 10 min; take the supernatant Put 5mL of liquid solution into a 50mL volumetric flask, dilute to the mark with PBS solution, and shake well; filter the glass fiber filter paper into a round bottom flask, and concentrate the filtrate to dryness with rotary evaporation, the vacuum degree of rotary evaporation is -0.09MPa, and the temperature is 60°C; add 2mL of absolute ethanol and ultrasonically dissolve, pass through a 0.45μm filter membrane to obtain a sample solution. Wherein, the PBS solution configuration method is the same as that in Example 1.

[0032] (2) Detection: 10 μL of the sample solution was taken and detected by liq...

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Abstract

The invention discloses a method for detecting ochratoxin A in capsicum extract. The method comprises the following steps: (1) performing ultrasonic extraction on a capsicum extract sample by using an alcoholic solution, so as to obtain an extracting solution; (2) freezing and centrifugally separating the extracting solution, so as to obtain the supernatant; (3) diluting the supernatant by using a PBS solution, and filtering to obtain the filtered liquid; (4) concentrating the filtered liquid to be dry, and adding a solvent for ultrasonic dissolving, so as to obtain a dissolving solution; (5) detecting the content of the ochratoxin A in the dissolving solution by adopting a liquid chromatography-fluorescence method. According to the method, toxins can be fully extracted from the sample, the impurities are removed by freezing and centrifuging, the sample is further purified by a PBS solution, an immunoaffinity column is not used, batch sample treatment is facilitated, and the effects of improving the detection efficiency, reducing the cost, saving energy and reducing consumption can be achieved. According to the method, the extracting solution is diluted and filtered by PBS and directly concentrated and detected, the detection limit is 1 [mu]g / kg, and the detection requirement is met.

Description

technical field [0001] The invention belongs to the field of food safety detection, in particular to a method for detecting ochratoxin A in pepper extract. Background technique [0002] Ochratoxins are a group of structurally similar toxic metabolites produced by toxin-producing strains such as Aspergillus and Penicillium, which are widely found in various foods, feeds and other agricultural by-products. Ochratoxins include 7 compounds with similar chemical structures, among which ochratoxin A (ochratoxin A, OTA) is the most widely distributed in nature, the most toxic, and has the greatest impact on humans, animals and plants. Toxicological studies have shown that ochratoxin A has renal toxicity, liver toxicity, immunotoxicity, teratogenicity, carcinogenicity and mutagenicity, etc., and has great potential harm to animal and human health. Therefore, all countries in the world attach importance to the detection and control of ochratoxin A, and have formulated relevant limit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14G01N2030/062
Inventor 张玉芬杨清山冯默张欢欢翟彦伟
Owner CHENGUANG BIOTECH GRP CO LTD
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