Application of pilose antler peptide in promoting proliferation of mesenchymal stem cells
A technology of stem cells and velvet antler polypeptide, applied in the field of biomedicine, can solve the problems of high price of cytokines, difficult to widely use, etc., and achieve the effect of significant effect, promoting the repair of kidney damage and promoting the proliferation of ADSCs cells.
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Embodiment 1
[0032] The effect of different concentrations of velvet antler polypeptide on the cell proliferation was detected by MTT method after 24 hours of action on the cells.
[0033] The first step: cell culture. Cell culture uses low-sugar DMEM medium with 10% serum content, using 75cm 2 Cell culture flasks, at 37°C, 5% CO 2 cultured in an incubator.
[0034] Step Two: Cell Digestion. Adipose-derived mesenchymal stem cells (ADSCs) in good growth condition in the logarithmic growth phase were taken and digested routinely. When digesting, first add 4ml PBS to wash the adipose-derived mesenchymal stem cells (ADSCs), wash twice, wash away the residual medium, and prevent the neutralization of the residual medium and trypsin. Then add 4ml of trypsin, put in 37 ℃, 5% CO 2 Digest in an incubator for 1 min. When the cells shrank, an equal amount of culture medium was added to terminate the digestion. Blow the wall of the cell flask with a disposable sterile plastic tube to blow the c...
Embodiment 2
[0043] The effect of different concentrations of velvet antler polypeptide on the cell proliferation was detected by MTT method after 48h of action on the cells.
[0044] The first step: cell culture. Cell culture uses low-sugar DMEM medium with 10% serum content, using 75cm 2 Cell culture flasks, at 37°C, 5% CO 2 cultured in an incubator.
[0045] Step Two: Cell Digestion. Adipose-derived mesenchymal stem cells (ADSCs) in good growth condition in the logarithmic growth phase were taken and digested routinely. When digesting, first add 4ml PBS to wash the adipose-derived mesenchymal stem cells (ADSCs), wash twice, wash away the residual medium, and prevent the neutralization of the residual medium and trypsin. Then add 4ml of trypsin, put in 37 ℃, 5% CO 2 Digest in an incubator for 1 min. When the cells shrank, an equal amount of culture medium was added to terminate the digestion. Blow the wall of the cell flask with a disposable sterile plastic tube to blow the cells ...
Embodiment 3
[0054] The effect of different concentrations of velvet antler polypeptide on cell proliferation was detected by MTT method after 72 hours of action on cells.
[0055] The first step: cell culture. Cell culture uses low-sugar DMEM medium with 10% serum content, using 75cm 2 Cell culture flasks, at 37°C, 5% CO 2 cultured in an incubator.
[0056] Step Two: Cell Digestion. Adipose-derived mesenchymal stem cells (ADSCs) in good growth condition in the logarithmic growth phase were taken and digested routinely. When digesting, first add 4ml PBS to wash the adipose-derived mesenchymal stem cells (ADSCs), wash twice, wash away the residual medium, and prevent the neutralization of the residual medium and trypsin. Then add 4ml of trypsin, put in 37 ℃, 5% CO 2 Digest in an incubator for 1 min. When the cells shrank, an equal amount of culture medium was added to terminate the digestion. Blow the wall of the cell flask with a disposable sterile plastic tube to blow the cells off...
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