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Tissue culture method of rhizoma atractylodis

A technique of tissue culture and Atractylodes atractylodes, applied in the field of Atractylodes atractylodes seedlings, can solve the problems of low reproduction coefficient, easy production of endophytic bacteria, difficulty in explant disinfection, etc., and achieve the effect of improving the induction rate

Inactive Publication Date: 2017-02-01
李志勇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are research reports on Atractylodes atractylodes tissue culture at present, there are problems such as difficult disinfection of explants, easy generation of endophytic bacteria, low reproduction coefficient, etc., and the present invention has the characteristics of simplicity, economy, and high operability, and effectively improves the Bud induction rate, subculture coefficient and rooting rate, laying the foundation for its large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Disinfection of explants: Take the stem tip of the strong Atractylodes atractylodis in the same year, scrub it clean, soak it in washing powder solution for 6 minutes, then gently scrub the surface of the explant with a soft brush to remove the dust and some bacteria on the surface, Then rinse with tap water for 2 hours and place it in an ultra-clean workbench, first disinfect with 75% ethanol for 6 seconds, then wash with sterile water for 4 times, then disinfect with 0.2% mercury liter solution for 12 minutes, rinse with sterile water for 5 times, and then use sterile filter paper Wipe off any water droplets on the surface and set aside.

[0019] (2) Start-up culture: Inoculate the shoot tip treated in step (1) into the start-up medium for bud induction. After inoculation, culture it in total darkness at 26°C for 5 days, and then the contamination rate is as low as 5%, and the survival rate reaches 80%. %. Then it was illuminated for 12 hours every day, and the l...

Embodiment 2

[0025] (1) Disinfection of explants: take the stem tips of the robust Atractylodis atractylodis in the same year, scrub them clean, soak them in washing powder solution for 8 minutes, and then gently scrub the surface of the explants with a soft brush to remove the dust and some bacteria on the surface. Then rinse with tap water for 3 hours and place it in an ultra-clean workbench, first disinfect with 75% ethanol for 11 seconds, then wash with sterile water for 6 times, then disinfect with 0.1% mercury liter solution for 14 minutes, rinse with sterile water for 6 times, and then use sterile filter paper Wipe off any water droplets on the surface and set aside.

[0026] (2) Start-up culture: Inoculate the shoot tips treated in step (1) into the start-up medium for bud induction. After inoculation, culture them in total darkness at 26°C for 4 days, and then the pollution rate is as low as 6%, and the survival rate reaches 82%. %. Then it was placed under the condition of 9 hou...

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Abstract

The invention discloses a tissue culture method of rhizoma atractylodis. The tissue culture method is characterized by being a method for rapidly obtaining a large number of tube seedlings of a good rhizoma atractylodis variety within a short time by a plant tissue culture technology. By the tissue culture method, with shoot tips of the rhizoma atractylodis as explants, through processes of sterilizing the explants, performing start culture, performing proliferation culture, performing seedling strengthening culture, performing rooting culture, hardening seedlings, transplanting and the like, the shoot inducing rate, the subculture coefficient and the rooting rate are effectively improved, and thus an intact tissue culture system for rhizoma atractylodis is established.

Description

technical field [0001] The invention relates to the technical field of Atractylodes atractylodis seedling propagation, and is characterized by a method for culturing Atractyrrhizae tissue. Background technique [0002] Cangzhu is also known as Mao Cangzhu, Northern Cangzhu, Yishu, and Southern Cangzhu. Morphological characteristics are perennial herbs, underground rhizomes are nodular cylindrical or lumpy, 1-4 cm in diameter, and the surface is gray or dark brown. Stems erect, 30-80 cm high, with sparse spider-like pores or no pores, alternate leaves, middle and lower stems and leaves 8-12 cm long, 5-8 cm wide, large head pinnately parted or half-parted, lateral lobes 1~4 pairs, elliptic, oblong or obovate elliptic, 0.5~2 cm wide, top lobe 1.5~4.5 cm wide, upper leaf base has 1~2 pairs of triangular lobes, all The leaves are glabrous, hard in texture, with needle-like hairs or triangular thorns on the edge. Blooms from June to October, white or purple-blue flowers, formin...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李志勇
Owner 李志勇
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