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Method for cultivating mother strain of nodule bacteria

A cultivation method and technology of rhizobia, applied in the field of cultivating rhizobia mother species, can solve the problems of low nodulation rate of inoculation of leguminous plants, poor adaptability of rhizobia, etc., and the requirements are not strict, the adaptability is good, and the production cost is low. low effect

Active Publication Date: 2017-02-01
SHANDONG WEIFANG ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

[0003] The purpose of the present invention is to provide a kind of cultivation method of rhizobia parent species for the deficiencies in the prior art, better solve the problems of poor adaptability of rhizobia and low nodulation rate of inoculation of leguminous plants, and make the effect of rhizobia fully play , the product meets the requirements of organic agricultural products, with low cost, high yield and good benefit

Method used

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Experimental program
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Embodiment

[0026] Embodiment, a kind of cultivation method of Rhizobium mother species, comprises the steps:

[0027] 1. Strain selection. The standards for the strain land are: ①Long-term production of similar leguminous crops, the old garden soil where the leguminous crops have been planted for no less than 5 years is used as the strain selection object, and the bean crops in the previous stubble have good nodules; ②No soil transmission Or epidemic diseases and insect pests, and no fungicides have been used recently; ③The type of soil and substrate is similar to the target environment (soil, substrate), and the relative difference between the main physical and chemical indicators such as organic matter content, PH value, and electrical conductivity does not exceed 50%; ④Soil samples To be as fresh as possible, long-term storage or long-distance transportation should maintain a low temperature of 5-10°C and a relative humidity of 60-90%.

[0028] Take more than 15cm of mellow soil, who...

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Abstract

The invention relates to a method for cultivating a mother strain of nodule bacteria. The method comprises a strain domesticating and culturing step: carrying out inferior elimination and superior selection by virtue of methods of environmental screening, antagonism competition and survival of the fittest in a simulated target environment, so as to find and cultivate the strain matched with the target environment and the variety. By virtue of the method, the problems that the nodule bacteria are poor in adaptability and the inoculating scaffolding rates of leguminous plants are low are well solved, the action of the nodule bacteria is fully exerted, and a product meets the requirements of organic agricultural products and is low in cost, high in yield and good in benefit.

Description

technical field [0001] The invention relates to a cultivation method of rhizobia parent species. Background technique [0002] Leguminous crops have the characteristic of using rhizobia to nodulate and fix nitrogen. Under suitable conditions, the nitrogen fixation of the crop itself can meet 50-70% of the demand. However, rhizobia have the characteristics of species and environment specificity, especially in legumes and vegetables. In production, there are common phenomena that legumes and vegetables do not form nodules and the effect of rhizobia fertilizer is poor. Especially when soilless cultivation is carried out on a sterilized substrate, the existing bacterial fertilizer has almost no effect. Tracing it to its cause is the problem of bacterial strain adaptability, furthermore, it is the problem of mother species breeding method. The existing rhizobia fertiliser species adopts the cultivation path of "bacteria collection-medium cultivation-dominant strain screening-d...

Claims

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Application Information

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IPC IPC(8): A01G1/00
Inventor 王本明林秀花宋银行郎文培李付军杨红光于海涛
Owner SHANDONG WEIFANG ACADEMY OF AGRICULTURAL SCIENCES
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