Manufacturing method of kidney specificity administration model

Abstract

The invention discloses a method for making a kidney-specific drug delivery model. Compared with the prior art, the invention facilitates the reverse diffusion of the drug or the virus vector carrying the target gene through the renal pelvis, and uniformly reaches all the kidneys of the used nephron. Small tubules and glomerular capsules, drugs or target genes form a uniform therapeutic high concentration in the local kidney, and have less impact on extrarenal tissues and organs. It is an ideal kidney-targeted drug delivery method and is conducive to efficient research on chronic kidney disease. Therapeutic targets and pathogenesis. Compared with other current methods, it has the advantages of simple operation, good organ specificity, uniform distribution of drug (gene) in organs, controllable concentration, and small extrarenal impact.

Description

technical field [0001] The invention relates to the construction of a chronic kidney disease model, in particular to a method for making a kidney-specific drug delivery model. Background technique [0002] With the development of social economy and the change of people's lifestyle, chronic kidney disease (CKD) has become an epidemic trend, and the incidence rate in the population is as high as 10%. Given that people's understanding of the pathogenesis of CKD is not deep enough, and there is a lack of satisfactory clinical intervention methods, most patients eventually enter the stage of end-stage renal disease, requiring blood purification and kidney transplantation, which brings a heavy burden to families and society. Tubulointerstitial fibrosis is an important histological manifestation of CKD. The deterioration of renal function depends largely on the degree of tubulointerstitial fibrosis. The unilateral ureteral obstruction (UUO) model is an experimental nephropathy mo...

AI Technical Summary

Problems solved by technology

However, the current UUO model still has some shortcomings

[0003] 1. At present, once the UUO mouse model is established, the obstruction in the body cannot be relieved, which is not conducive to the observation of the treatment effect of the disease

[0004] 2. For the treatment of CKD, systemic drug administration is basically used to observe the recovery effect of renal function. There is no effective local drug therapy for the kidney

Ultrasonic transfection is the transfection of genes into the body through ultrasonic waves. Ultrasonic transfection is only suitable for animals and is not easy to operate, and strong ultrasonic waves can cause damage to surrounding normal tissues

High-pressure injection transfection is to inject a high-volume liquid containing the target gene into the body instantaneously, resulting in a transient high perfusion pressure, so that the target gene enters the cell; high-pressure injection transfection will cause instantaneous hypertension and high volume load in the body, It is extremely harmful to the body, which limits its popularization and application

[0006] It is the lack of animal models that can be widely used and recognized for the treatment of obstructive nephropathy that prevents rapid progress in research in this field and greatly limits the development of clinical intervention methods