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Compound microbial preparation for preventing brown spot of Cassava and preparing method thereof

A technology of microbial preparations and composite microorganisms, applied in botany equipment and methods, biocides, chemicals for biological control, etc., can solve the problems of endangering the environment, human physical and mental health, long breeding cycle, and low efficiency. Achieve super-strong control of cassava brown spot, highly targeted, and low-use effects

Inactive Publication Date: 2017-01-18
FOSHAN YANHUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional chemical control, although chemical control has the advantages of quick results and high efficiency, it not only endangers the environment and human physical and mental health, but also increases the resistance of pathogenic bacteria and changes the physiological race of pathogens.
Breeding for resistance to diseases and insect pests, using the resistance of crops to control pests and diseases is also a key research direction, but the breeding cycle is long, the efficiency is low, and it cannot adapt to the rapid development of diseases; the second is biological control, due to the efficacy of biological control High, long-lasting, not easy to produce drug resistance, environmental friendliness and other advantages are increasingly favored by people
However, there is no relevant report on biological agents against cassava brown spot

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A microbial preparation for preventing and treating cassava brown spot disease. The raw material ratio of the microbial preparation is: 20 parts of Streptomyces Durham fermentation broth, 30 parts of Streptomyces russsa fermentation broth, 20 parts of Bacillus terescens fermentation broth, Barcelona type 20 parts of Bacillus fermentation liquid invaded 20 parts of Trichoderma fermentation liquid.

[0037] The preparation method of the compound microbial preparation of this control cassava brown spot disease, comprises the following steps:

[0038] Step 1: Preparation of Fermented Broth of Streptomyces Durham

[0039] Take out the preservation tube of Streptomyces durham, scratch the plate with Gaoshi No. 1 solid medium for recovery, and culture at 30°C for 7 days. Pick a single colony under the plate and inoculate it into an eggplant bottle with 150 ml of Gaoshi No. 1 solid medium, and culture it in an incubator at 30°C for 6-8 days. When the lawn is overgrown with the...

Embodiment 2

[0063] Example 2 Application effect of compound microbial preparation in cassava field

[0064] 1. The test was selected to be carried out in high-yield cassava production areas;

[0065] 2. The test location is Wuming County, Nanning City, Guangxi Autonomous Region; in the same piece of land with obvious cassava brown spot disease,

[0066] 3. Drug usage and dosage:

[0067] Test group: after the occurrence of brown spot disease, spray 2L of the compound microbial preparation per mu on the foliage, the dilution factor is 100-200 times, use it twice continuously, and use it once every 15 days;

[0068] Chemical agent group: after the occurrence of brown spot disease, spray with carbendazim as the control agent, and the area used for each group is 2 mu;

[0069] Clean water control group: spray with clean water;

[0070] The spraying equipment is a manual knapsack sprayer commonly used by farmers, and other management measures are the same;

[0071] 4, test result: as can be ...

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PUM

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Abstract

The invention discloses a compound microbial preparation for preventing brown spot of Cassava and a preparing method thereof. The raw materials of the compound microbial preparation comprises fermentation liquor of streptomyces Durham, fermentation liquor of streptomyces Russa, fermentation liquor of mud Bacillus licheniformis, fermentation liquor of Paenibacillus Barcelona, and fermentation liquor of Trichoderma aggression. According to the compound microbial preparation for preventing brown spot of Cassava, cultures can make full use of antibiosis, competition, hyperparasitism, bacteriolysis of interspecific or intraspecific microorganism; and through secondary metabolite, Trichoderma is induced to generate disease resistance, which can produce multiple antibiotics such as durhamycin, antifungal pentaene antibiotics, and antibiotics which can produce antifungal tetraene; russa mycin is used to prohibit pathogenic bacteria in brown spot of Cassava, and improves disease preventing effect of Cassava. The Compound microbial preparation for preventing brown spot of Cassava is environmental friendly and safe to people and livestock, and can not easily generate drug resistance; meanwhile, the preparing method is simple in procedures, low in cost and easy in using.

Description

technical field [0001] The invention relates to the technical field of microbial application, in particular to a compound microbial preparation for preventing and treating cassava brown spot and a preparation method thereof. Background technique [0002] Brown spot disease is a worldwide disease in cassava production, which widely occurs in cassava producing areas in Asia, Africa and America, seriously affecting the yield and quality of cassava. [0003] Cassava brown spot disease is caused by the pathogenic bacteria Cercospora cassava, which belongs to the subphylum Deuteromycota, Rhizoctonia, Rhizoctonia, Pleurobacteriaceae, and Cercospora. The fungus parasitizes in the intercellular canal of the leaves, forming a sub-base with a depth of 2-6 layers of cells and a diameter of 20-45 μm. The conidiophores grow in clusters on the sub-base. Conidiophores light brown (mostly close to light black), uniform in color and width, unbranched, 0-2 geniculate in the middle, rounded at...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01P3/00A01P21/00
CPCA01N63/00
Inventor 蒋常德胡艳晖
Owner FOSHAN YANHUI BIOTECH CO LTD
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