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Chiral analysis method for nicotine in cigarette cut tobacco

An analysis method and nicotine technology, which are applied in the field of tobacco chemical composition determination, can solve the problems that cannot meet the needs of nicotine optical isomer analysis, cannot be applied to the analysis of nicotine chirality of cigarette cut tobacco, and improve the pretreatment effect. , recovery rate and repeatability, and the effect of low detection limit

Inactive Publication Date: 2017-01-11
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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Problems solved by technology

Considering that the ratio of R-(+)-nicotine in tobacco is about 0.2-0.3%, this patented method cannot meet the needs of the analysis of nicotine optical isomers in cigarette shreds
Liu et al. used multidimensional gas chromatography to analyze the chiral composition of nicotine in cigarette smoke. The detection limit of R-(+)-nicotine in this method was 0.5% (Liu, etc. Journal of Analytical & Applied Pyrolysis, 81, 157 -161. 2008.), this method is also not applicable to the analysis of chirality of nicotine in cigarette cut tobacco
In addition, the development of very low nicotine content cigarettes in recent years has made the nicotine content in this type of cigarettes about 95% lower than that of "light" cigarettes, which brings new challenges to the chiral analysis of nicotine

Method used

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  • Chiral analysis method for nicotine in cigarette cut tobacco
  • Chiral analysis method for nicotine in cigarette cut tobacco

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Embodiment Construction

[0032] The present invention is further described by the following specific examples, but does not limit the present invention.

[0033] 1. Instruments and Reagents

[0034] MDGCMS-QP2010Ultra multidimensional gas chromatography spectrometer (Shimadzu Corporation), autosampler AOC-5000 (Shimadzu Corporation), automatic solid phase microextraction fiber injection needle, 65 μm PDMS / DVB (polydimethylsiloxane) Oxane / divinylbenzene) stationary phase extraction fiber head (Supelco, USA), Rtx-5MS chromatographic column (30 m × 0.25 mm × 0.25 μm, Japan Shimadzu Corporation), InertCap CHIRAMIX chromatographic column (30 m × 0.25 mm × 0.25 μm, Japan Shimadzu Corporation), AE163 electronic balance (sensitivity: 0.0001 g, Switzerland Mettler Company), HY-8 speed-regulating oscillator (Changzhou Guohua Electric Co., Ltd.), high-speed pulverizer (Wuhan Yincai Technology Co., Ltd. ), Germany SIGMA 3-30K-high-speed desktop refrigerated centrifuge. n-Hexane (chromatographically pure), sodiu...

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Abstract

The invention discloses a chiral analysis method for nicotine in cigarette cut tobacco. The chiral analysis method for nicotine in cigarette cut tobacco is characterized by including the steps that cut tobacco samples are dried, smashed and screened, extracting liquid is added, the mixture is extracted and filtered, then common cut-tobacco-sample extracting liquid is directly analyzed through a multidimensional gas phase chromatography and mass spectrometry detector, cut-tobacco-sample extracting liquid with the excessively-low nicotine content is subjected to automatic solid phase microextraction to be subjected to multidimensional gas-phase-chromatography tandem-mass-spectrum analysis, and the proportion of S-(-)-nicotine and R-(+)-nicotine in the cigarette cut tobacco is quantitatively detected with the peak area normalization method. The detection method is simple, fast and convenient in sample treatment, low in method detection limit, high in sensitivity and good in accuracy degree, and analysis of cigarette cut tobacco samples can be met.

Description

technical field [0001] The invention relates to a method for measuring chemical components of tobacco, in particular to a chiral analysis method for nicotine in shredded tobacco of cigarettes. Background technique [0002] Nicotine is the most important pharmacological component of tobacco and one of the most important reasons why people consume tobacco. The nicotine molecule contains a chiral center, which is the 2-position carbon atom on the tetrahydropyrrole ring, so nicotine has two enantiomers: S-(-)-nicotine and R-(+)-nicotine. In tobacco, the two enantiomers have completely different amounts, mechanisms of metabolism and physiological properties. The content of S-(-)-nicotine in tobacco is much higher than that of R-(+)-nicotine. R-(+)-nicotine accounts for 0.2-0.3% of the total nicotine content in tobacco. Due to the existence of heating The isomers in the process are reversed, and the content of R-(+)-nicotine in cigarette smoke can reach about 3%, but it is still...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 付亚宁韩书磊刘彤陈欢侯宏卫胡清源
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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