Method for preparing butanedioic acid

A technology of succinic acid and sodium succinate, applied in the field of bioengineering, can solve the problems of high loss rate of succinic acid, large amount of activated carbon, low purity of succinic acid products, etc., and achieve the effect of convenient regeneration

Inactive Publication Date: 2017-01-11
SINOPEC YANGZI PETROCHEM +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN101486637A discloses a process for extracting succinic acid from fermented liquid by electrodialysis, but this method consumes a lot of energy, and the membrane module is easy to pollute, so it is

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The strain used for fermentation: Actinobacillus succinogenes NJ113.

[0029] Seed medium (g / L): glucose 10, yeast extract 5, NaHCO 3 10, NaH 2 PO 4 2H 2 O2,K 2 HPO 4 ·3H 2 O 3 peptone 2.

[0030] Fermentation medium (g / L): glucose 80, yeast extract 10, corn steep liquor 5, sodium acetate 1, KH 2 PO3, MgCl 2 ·6H 2 O 0.3, anhydrous CaCl 2 0.3.

[0031] Fermentation tank culture: put the activated seed liquid and the sterilized glucose into the fermentation tank, the inoculum size is 7%, (v / v), the stirring speed is 100rpm, 37 ℃ fermentation culture, CO 2 The ventilation rate is 0.1vvm, and the pH is controlled at 6.2 during the whole fermentation process. The fermented liquid containing sodium succinate obtained with sodium carbonate as a pH regulator, wherein the concentration converted into succinic acid is 60g / L, the concentration of formic acid is 5g / L, the concentration of acetic acid is 11g / L, and there is no residual sugar, The broth was adjusted to p...

Embodiment 2

[0033] The strain used for fermentation: Actinobacillus succinogenes NJ113.

[0034] Seed medium (g / L): glucose 10, yeast extract 5, NaHCO 3 10, NaH 2 PO 4 2H 2 O2,K 2 HPO 4 ·3H 2 O 3 peptone 2.

[0035] Fermentation medium (g / L): glucose 80, yeast extract 10, corn steep liquor 5, sodium acetate 1, KH 2 PO3, MgCl 2 ·6H 2 O 0.3, anhydrous CaCl 2 0.3.

[0036]Fermentation tank culture: put the activated seed solution and the sterilized glucose into the fermentation tank, the inoculum size is 7%, (v / v), the stirring speed is 200rpm, 37 ℃ fermentation culture, CO 2 The ventilation rate is 0.1vvm, and the pH is controlled at 6.8 during the whole fermentation process. The fermented liquid containing sodium succinate obtained with sodium carbonate as a pH regulator, wherein the concentration converted into succinic acid is 60g / L, the concentration of formic acid is 5g / L, the concentration of acetic acid is 11g / L, and there is no residual sugar, The pH of the fermentation...

Embodiment 3

[0038] The strain used for fermentation: Actinobacillus succinogenes NJ113.

[0039] Seed medium (g / L): glucose 10, yeast extract 5, NaHCO 3 10, NaH 2 PO 4 2H 2 O2,K 2 HPO 4 ·3H 2 O 3 peptone 2.

[0040] Fermentation medium (g / L): glucose 80, yeast extract 10, corn steep liquor 5, sodium acetate 1, KH 2 PO3, MgCl 2 ·6H 2 O 0.3, anhydrous CaCl 2 0.3.

[0041] Fermentation tank culture: put the activated seed liquid and the sterilized glucose into the fermentation tank, the inoculum size is 7%, (v / v), the stirring speed is 300rpm, 37 ℃ fermentation culture, CO 2 The ventilation rate was 0.1vvm, and the pH was controlled at 7.0 throughout the fermentation process. The fermented liquid containing sodium succinate obtained with sodium carbonate as a pH regulator, wherein the concentration converted into succinic acid is 60g / L, the concentration of formic acid is 5g / L, the concentration of acetic acid is 11g / L, and there is no residual sugar, The pH of the fermentation ...

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PUM

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Abstract

The invention provides a method for preparing butanedioic acid and belongs to the field of microbes. The method comprises the following specific steps: (1) carrying out culture by a microbial fermentation method, so as to obtain sodium succinate fermentation liquor; and (2) adjusting the pH value of the butanedioic acid containing fermentation liquor, carrying out solid-liquid separation on the butanedioic acid containing fermentation liquor, removing part of proteins and pigments so as to obtain clarified fermentation liquor, subjecting the fermentation liquor to concentrating and crystallizing, then, dissolving crystals, carrying out activated-carbon decoloring on the dissolved liquid so as to obtain decolored fermentation liquor, and subjecting the obtained decolored fermentation liquor to acidifying, crystallizing and baking, thereby obtaining the butanedioic acid. The traditional calcium salt method, liquid-liquid extraction method and electrodialysis method all have some disadvantages. According to the method, a novel butanedioic acid extraction process is developed on the basis of a membrane separation technology; and compared with the prior art, the method has the advantages that the decoloring cost is reduced relatively substantially, and the purity of the obtained butanedioic acid product can reach 99.8% to the maximum.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for separating and extracting sodium succinate from fermentation broth. Background technique [0002] Succinic acid (Succinic Acid, Butanedioic Acid), also known as succinic acid, is recognized by the US FDA as "generally recognized as safe" (GRAS), so it can be used for a variety of purposes. A 2004 report by the U.S. Department of Energy pointed out that succinic acid is a platform chemical substance for the synthesis of bulk daily necessities and important chemicals, and has huge economic value. With the shortage of energy, the prospect of producing succinic acid by biological fermentation is very broad. [0003] A number of technologies have been developed to convert succinic acid into important industrial chemicals, including N-methylpyrrolidone, 1,4-butanediol, tetrahydrofuran, γ-butyrolactone, adipic acid, PBT resin, PBS degradable materials, ...

Claims

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Application Information

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IPC IPC(8): C07C55/10C07C51/42
CPCC07C51/42C07C55/10
Inventor 方晓江刘经伟陈韶辉杨爱武
Owner SINOPEC YANGZI PETROCHEM
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