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Preparation method for diluent for milk goat artificial insemination technology

A technology for diluting and milking goats, applied in the biological field, can solve the problems of low sperm motility, low semen dilution ratio, short sperm survival time, etc., and achieve the effects of simple configuration method, improved breeding economic benefits, and easy to master.

Inactive Publication Date: 2017-01-11
XIAN LEMIN RUMINANT INST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a preparation method of diluent for artificial insemination of dairy goats, to overcome the low semen dilution factor of existing dairy goat diluents, short sperm survival time after semen dilution, low sperm motility after dilution and artificial insemination. Low conception rate of ewes

Method used

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  • Preparation method for diluent for milk goat artificial insemination technology

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preparation example Construction

[0017] The invention provides a method for preparing a diluent for artificial insemination of dairy goats, the specific steps of which are as follows:

[0018] Step 1, configure the base liquid

[0019] At an ambient temperature of 35-40°C, weigh 3-5g of glucose, 1-2g of sodium citrate and 0.05-0.2g of disodium edetate, pour these three substances into a container, and pour Add distilled water to the container to 100ml, stir evenly to obtain a mixed solution; then add enrofloxacin to the mixed solution, stir evenly to obtain a base liquid; add 5000UG enrofloxacin to every 100mL mixed solution; Store at a low temperature of 4°C;

[0020] Step 2, configure the protection solution

[0021] Under the condition that the ambient temperature is 35-40°C, configure an ethylene glycol solution with a mass fraction of 10%-15%; then add vitamin C and vitamin B to the ethylene glycol solution 12 , oxytocin and prostaglandins; add 0.03-0.10ml vitamin C and 0.01-0.5ml vitamin B per 1ml et...

Embodiment 1

[0031] A method for preparing a diluent for artificial insemination of dairy goats, the specific steps of which are as follows:

[0032] Step 1, configure the base liquid

[0033] At an ambient temperature of 40°C, weigh 3g of glucose, 2g of sodium citrate and 0.1g of disodium edetate, pour these three substances into a container, add distilled water to the container to 100ml, and stir uniform to obtain a mixed solution; then add enrofloxacin to the mixed solution and stir evenly to obtain a base solution; add 5000UG enrofloxacin to each 100mL mixed solution; finally store the base solution at a low temperature of 4°C;

[0034] Step 2, configure the protection solution

[0035] Under the condition that the ambient temperature is 40°C, configure an ethylene glycol solution with a mass fraction of 11%; then add vitamin C and vitamin B to the ethylene glycol solution 12 , oxytocin and prostaglandins; add 0.1ml vitamin C and 0.15ml vitamin B per 1ml ethylene glycol solution 12 ...

Embodiment 2

[0041] A method for preparing a diluent for artificial insemination of dairy goats, the specific steps of which are as follows:

[0042] Step 1, configure the base liquid

[0043] At an ambient temperature of 35°C, weigh 3.5g of glucose, 1.5g of sodium citrate and 0.05g of disodium edetate, pour these three substances into a container, and add distilled water to the container to 100ml , stir evenly to obtain a mixed solution; then add enrofloxacin to the mixed solution, and stir evenly to obtain a base solution; add 5000UG enrofloxacin to each 100mL mixed solution; finally store the base solution at 4°C at low temperature;

[0044] Step 2, configure the protection solution

[0045]Under the condition that the ambient temperature is 35°C, configure an ethylene glycol solution with a mass fraction of 13%; then add vitamin C and vitamin B to the ethylene glycol solution 12 , oxytocin and prostaglandins; add 0.08ml vitamin C and 0.5ml vitamin B per 1ml ethylene glycol solution ...

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Abstract

The invention provides a preparation method for a diluent for the milk goat artificial insemination technology. The method comprises the steps that glucose, sodium citrate and disodium edetate dehydrate are poured into a container, distilled water is added into the container, and enrofloxacin is added into the mixed solution to obtain a basic solution; vitamin C, vitamin B12, oxytocin and prostaglandin are added into an ethylene glycol solution to obtain a protective solution; then, kanamycin sulfate is added into skimmed goat milk to obtain a nutrient solution; finally, the basic solution, the protective solution and the nutrient solution are mixed and stirred to be uniform under the sterile condition at low temperature of 4 DEG C to obtain the diluent for the milk goat artificial insemination technology. The preparation method has the advantages that the semen dilution ratio can reach up to 30 times, sperm activity can still be kept at 0.3-0.4 120 h after semen is diluted, and it can be ensured that the ewe artificial insemination hybridization pregnancy rate is 85% or above.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preparing diluent for artificial insemination of dairy goats. Background technique [0002] Semen cryopreservation and artificial insemination technology play a pivotal role in modern animal husbandry production. The use of frozen semen for artificial insemination can not be limited by time, region, variety and individual, and can greatly reduce the number of male animals and reduce production costs. Improve the utilization rate of high-quality breeding males and improve the economic benefits of the breeding industry. The application of frozen semen technology and artificial insemination technology on dairy cows and beef cattle in my country has been relatively well developed. In the process of promoting artificial insemination technology for dairy goats, the diluent for artificial insemination of dairy goats on the market is not effective, making it difficult to promote...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226A01N1/0215A01N1/0221
Inventor 王光亚周敏娟黄耀辉何泰
Owner XIAN LEMIN RUMINANT INST
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