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Method for simultaneously extracting oil and protein and/or glucoside in moringa seeds

A technology of Moringa seed oil and Moringa seed, which is applied in the direction of fat oil/fat production, fat production, fermentation, etc., can solve the problems of insufficient comprehensive utilization and low extraction rate of Moringa seed oil, and achieve easy promotion and comprehensive utilization Value, the effect that the health care function is strong

Inactive Publication Date: 2017-01-04
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to provide a method for simultaneously extracting oil, protein and / or glycosides in Moringa seeds in view of the technical insufficiency of providing multiple components in Moringa seeds at the same time in the prior art, and simultaneously solve the problem of Moringa seeds Low oil extraction rate, insufficient comprehensive utilization of protein and carbohydrates, etc.

Method used

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  • Method for simultaneously extracting oil and protein and/or glucoside in moringa seeds
  • Method for simultaneously extracting oil and protein and/or glucoside in moringa seeds
  • Method for simultaneously extracting oil and protein and/or glucoside in moringa seeds

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Experimental program
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Effect test

Embodiment 1

[0075] Embodiment 1 Grease and crude protein preparation

[0076] S1. Extract Moringa oleifera seed oil: Dehull and pulverize moringa oleifera seeds and use subcritical fluid extraction to extract oil and get moringa oleifera seed oil and residue I; the extraction agent is butane, and the ratio of solid to liquid is 1:2 (g / mL), the extraction temperature is 30°C, the extraction time is 40min, and the extraction times are 2 times;

[0077] S2. Ultrasonic treatment: take the residue I after extracting Moringa oleifera seed oil in step S1, add a sodium hydroxide solution with a pH of 8.0 according to a solid-liquid ratio of 1:5 (g / mL), and then ultrasonically treat it at 300W and 50°C 15min.

[0078] S3. Enzymolysis: After ultrasonic treatment, the mixture is centrifuged to obtain supernatant and residue. Mix the residue with water at a ratio of 1:8 (g / mL). Cellulase was added to the mixed solution in an amount of 0.1% of the mass of the residue II, and then extracted at 30° ...

Embodiment 2

[0082] Embodiment 2 fat and crude protein preparation

[0083] S1. Extract Moringa oleifera seed oil: Dehull and pulverize Moringa oleifera seeds and use subcritical fluid extraction method for oil extraction to obtain Moringa oleifera seed oil and residue I; the extraction agent is propane, and the ratio of solid to liquid is 1:3 (g / mL ), the extraction temperature is 50°C, the extraction time is 20min, and the extraction times are 3 times;

[0084] S2. Ultrasonic treatment: take the residue I after extracting Moringa oleifera seed oil from S1, add sodium hydroxide solution with a pH of 9.0 according to a solid-liquid ratio of 1:10 (g / mL), and then ultrasonicate at 400W and 60°C for 20min.

[0085] S3. Enzymolysis: After ultrasonic treatment, the mixture is centrifuged to obtain supernatant and residue. Mix the residue with water at a solid-liquid ratio of 1:12 (g / mL). Protease was added to the mixture in an amount of 0.5% of the mass of the residue II, and then extracted a...

Embodiment 3

[0089] Embodiment 3 fat and crude protein preparation

[0090] S1. Extract Moringa oleifera seed oil; perform solvent extraction according to conventional methods to obtain oil and residue I.

[0091] S2. Ultrasonic treatment: Take the residue I after solvent extraction of Moringa oleifera seed oil, add sodium hydroxide solution with a pH of 10.0 according to a solid-liquid ratio of 1:15 (g / mL), and then ultrasonicate at 500W and 75°C for 15min.

[0092] S3. Enzymolysis: After ultrasonic treatment, the mixture is centrifuged to obtain supernatant and residue. Mix the residue with water at a solid-liquid ratio of 1:15 (g / mL). Add alkaline protease to the mixed solution first, and then add cellulase after enzymolysis for 1.5 hours. The total amount of the two enzymes added is 1.0% of the mass of residue II, and the ratio is alkaline protease: cellulase = 1:2 (w / w), and then stirred and extracted at 50° C. for 6.0 h to obtain an enzymatic hydrolysis solution.

[0093] S4. Enzy...

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Abstract

The invention discloses a method for simultaneously extracting oil and protein and / or glucoside in moringa seeds. Alkali liquor is added into residues obtained after moringa seed oil is extracted, after ultrasonic treatment and pertinent enzyme treatment are completed, treatment means such as membrane separation and isoelectric precipitation are taken, and crude protein and glucoside crude products are obtained after recovery is completed. According to the method, through organic combination of multiple optimizing treatment means, effective ingredients in the moringa seeds are efficiently extracted, and the oil extraction rate is larger than 90%, the protein and glucoside recovery rate can reach up to 85% or above. The moringa seed oil obtained through the method is high in quality, good in protein functional character and high in glucoside purity, and simultaneous extraction of oil, protein and glucoside in the moringa seeds is creatively achieved.

Description

technical field [0001] The invention relates to the field of deep processing and comprehensive utilization of woody oils, and more particularly relates to a method for simultaneously extracting oils, proteins and / or glycosides in Moringa oleifera seeds. Background technique [0002] Moringa, also known as Drumstick tree, is a perennial tropical deciduous tree native to northern India. There are about 14 species in the world. At present, there are two commonly eaten species: Indian traditional Moringa ( Moringaoleifera Lam.), African Moringa (Moringastenopetala). [0003] The seeds of Moringa oleifera, commonly known as Moringa seeds, have a crisp and sweet taste with a long-lasting sweetness. Moringa seeds have high nutritional value, containing 30% to 37% oil, 35% to 40% protein, 15% to 18% soluble sugar, and rich mineral elements. Regular consumption of Moringa seeds can enhance immunity, detoxification, body sculpting, anti-aging, anti-cancer, and has a great improvemen...

Claims

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Application Information

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IPC IPC(8): C12P21/06C12P19/14C12P19/00C11B1/06C11B1/10C11B1/00C11B1/04
CPCC11B1/00C11B1/04C11B1/06C11B1/104C12P19/00C12P19/14C12P21/06Y02P20/54
Inventor 吴雪辉龙婷
Owner SOUTH CHINA AGRI UNIV
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