Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method and application of mucor-fermented soybean bitter peptide

A technology of bitter peptide and fermented soya bean, which is applied in the field of preparation of mucormycin-type fermented fermented soya bean bitter peptide, can solve the problems of poor stability and low purity of bitter peptide, achieve the effect of easy chemical synthesis, high product purity, and broaden the application field

Active Publication Date: 2017-01-04
SOUTHWEST UNIVERSITY
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the above technical problems, the present invention provides a preparation method and application of a mucormycin-type tempeh bitter peptide, aiming at solving the problem of poor stability and low purity of the bitter peptide produced by the existing mucor-type fermented soybean bitter peptide preparation method The problem

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of mucor-fermented soybean bitter peptide
  • Preparation method and application of mucor-fermented soybean bitter peptide
  • Preparation method and application of mucor-fermented soybean bitter peptide

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0032] Such as figure 1 As shown, the preparation method of the Mucor type tempeh bitter peptide of the embodiment of the present invention comprises the following steps:

[0033] S101: Preparation of crude peptide: Accurately weigh a certain amount of tempeh sample (10.00g)→quick freezing in liquid nitrogen→grind into powder in the frozen state→add deionized water (such as 50mL) according to the ratio of material to liquid 1:8-16→ice bath Homogenize for 6min-12min→ultrasonic extraction at 60W-120W for 20min-40min→adjust pH to 5.5-6.5→stir at 45°C for 50min-90min→centrifuge (2000g) at 4°C for 15min-30min→take supernatant→repeat extraction once →Combine the two extracts→centrifuge (10500g) at 4°C for 15min-30min→dialyze the supernatant through a 100Da-200Da dialysis bag for 18h-30h→collect the extract and freeze-dry to obtain crude peptide powder A;

[0034] S102: Dissolve powder A in deionized water to make the concentration reach 5mg / mL-40mg / mL, use NKA-II macroporous resin ...

Embodiment 1

[0057] Preparation of crude peptide: Accurately weigh tempeh sample (10.00g)→quick freezing in liquid nitrogen→grind into powder in frozen state→add deionized water (50mL), homogenate in ice bath (8min)→ultrasonic extraction (80W, 30min)→adjust pH is 6.0→Stir (45°C, 60min)→Centrifuge (2,000g, 4°C, 20min)→Take supernatant→Repeat extraction once, combine two extracts→Centrifuge (10,500g, 4°C, 20min)→Up The clear liquid was dialyzed through a 100Da dialysis bag for 24 hours → the extract was collected and freeze-dried to obtain crude peptide powder A;

[0058] Powder A was dissolved in deionized water to make the concentration reach 5mg / mL, and purified by NKA-II macroporous resin column, first eluted with deionized water to equilibrium, and then eluted with 25% ethanol solution as eluent, Collect the eluted fractions, concentrate and lyophilize to obtain powder B;

[0059] Dissolve powder B in deionized water to reach a concentration of 5 mg / mL, then use Sephadex G-25 gel colum...

Embodiment 2

[0063] Preparation of crude peptide: Accurately weigh tempeh sample (20.00g)→quick freezing in liquid nitrogen→grind into powder in frozen state→add deionized water (320mL), homogenate in ice bath (12min)→ultrasonic extraction (120W, 30min)→adjust pH is 6.5→Stir (45℃, 90min)→Centrifuge (2,000g, 4℃, 30min)→Take supernatant→Repeat extraction once, combine two extracts→Centrifuge (10,500g, 4℃, 30min)→Up The clear liquid was dialyzed by 200Da dialysis bag for 32 hours → the extract was collected and freeze-dried to obtain crude peptide powder A;

[0064] Dissolve powder A in deionized water to make the concentration reach 40mg / mL, use NKA-II macroporous resin column for purification, first elute with deionized water to equilibrium, and then elute with 30% ethanol solution as eluent , collect the eluted fractions, concentrate and freeze-dry to obtain powder B;

[0065] Dissolve powder B in deionized water to reach a concentration of 40mg / mL, then use Sephadex G-25 gel column for p...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a preparation method and application of mucor-fermented soybean bitter peptide. The preparation method comprises the following steps of preparing powder A, powder B and powder C; dissolving the powder C into deionized water; by taking aqueous solution of A phase trifluoroacetic acid with the mass fraction of 0.05 percent and B phase trifluoroacetate acetonitrile with the mass fraction of 0.05 percent as eluent, performing gradient elution by adopting a semi-preparative HPLC (High Performance Liquid Chromatography) inverse phase C18 column, collecting a target peak, and performing freeze drying to obtain powder D; dissolving the powder D into the deionized water; by taking the aqueous solution of the A phase trifluoroacetic acid with the mass fraction of 0.05 percent and the B phase trifluoroacetate acetonitrile with the mass fraction of 0.05 percent as the eluent, performing gradient elution by adopting an analytical HPLC inverse phase C18 column, collecting a target peak, and performing freeze drying to obtain bitter peptide I. The prepared bitter peptide I is applied to food, and can be used for providing the bitterness of soybean in food, and increasing the delicate flavor of the food; the bitter peptide is easy for chemical synthesis; in addition, a synthesized product is high in purity and good in stability; the application range of the bitter peptide can be widened.

Description

technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a preparation method and application of a mucormycin-type tempeh bitter peptide. Background technique [0002] Mucoral fermented soya bean is a traditional fermented soy product with Chinese characteristics, which has a long history. Therefore, for thousands of years Mucor type fermented soya bean is deeply liked by Chinese sons and daughters always. With the development of the times, the production mode of Mucor-type Douchi has also become modernized, but our country lags behind Japanese natto and Korean soybean paste in the research on the bitter peptide of Mucor-type Douchi. How to learn from the successful experience of natto and soybean paste, and thoroughly study the structure and bitterness mechanism of the mucor-type tempeh bitter peptide is the focus of current research. [0003] Bitter peptide is an important component that affects the expression o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K1/20C07K1/16C07K1/14A23L27/20
CPCA23V2002/00C07K1/14C07K1/16C07K1/20A23V2200/16A23V2250/55
Inventor 阚建全李希宇夏雪娟丁涌波任廷远索化夷张甫生
Owner SOUTHWEST UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com