Method for extracting total anthraquinone from rheum officinale and product obtained through method

Abstract

The invention discloses a method for extracting total anthraquinone from rheum officinale. The method comprises the following steps of 1 extraction with ethanol, wherein rheum officinale is crushed, screened and extracted with ethanol, an ethanol extracting solution is recycled, centrifuging is conducted, supernatant liquid is collected, ethanol is extracted through vacuum concentration, and ethanol extracting paste of rheum officinale is obtained; 2 acidic hydrolysis, wherein the ethanol extracting paste of rheum officinale is heated for hydrolysis in an acidic aqueous solution, standing and suction filtration are conducted, and an obtained filter cake is a total aglycone extract; 3 alkali extraction and acid precipitation, wherein the total aglycone extract is dissolved with an alkaline aqueous solution, suction filtration is conducted, filtrate is collected, the pH of the filtrate is regulated to be acidic with an acidic aqueous solution, standing is conducted, precipitates are separated out, filtering is conducted, and an obtained filter cake is a total anthraquinone extract. The method is simple and feasible in technology route, low in production cost, short in period, high in safety coefficient and low in hazard coefficient to the environment and can achieve industrialized production.

Description

technical field [0001] The invention relates to a method for extracting active ingredients from rhubarb, in particular to a method for extracting total anthraquinones in rhubarb. Background technique [0002] The total anthraquinones of rhubarb are the main active ingredients of rhubarb, a traditional Chinese medicinal material. The total anthraquinones of rhubarb include free anthraquinones and conjugated anthraquinones, but free anthraquinones and conjugated anthraquinones have different pharmacological activities. Studies in recent years have also shown that long-term excessive application of rhubarb can cause liver and kidney damage, and its toxic components may also be the anthraquinones contained in it, but the toxicity of bound anthraquinones and free anthraquinones is also different. The latter, combined with anthraquinones, exert a laxative effect after intestinal degradation to free anthraquinones. Therefore, extracting total anthraquinones from rhubarb is more m...

AI Technical Summary

Problems solved by technology

At present, there are many patents and papers on the extraction of total anthraquinones in rhubarb reported in the literature, and the main problems include: 1. Most of the methods are only suitable for laboratory research, the operation steps are complicated, and the process route is difficult to achieve industrial production, such as using methanol extraction, Organic solvents or technologies with low safety factors such as chloroform extraction (including environmental and food safety); 2. A variety of chromatographic techniques are used in the separation process, such as macroporous adsorption resins, polyamide chromatography, etc., with high costs, complicated production routes, and poor quality. Difficult to control

Up to now, there is no report on the method of preparing high-purity rhubarb total anthraquinones with industrial value

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