Method for creating sesame mutants with EMS mutagenic agent

Abstract

The invention belongs to the technical field of sesame breeding, and particularly relates to a method for efficiently creating sesame mutants with an ethylmethane sulfonate (EMS) mutagenic agent on a large scale. The method includes the steps that sesame seeds are treated with the EMS mutagenic agent, the seeds are planted, seedlings are transplanted, and the mutants are obtained. According to the method, by optimizing EMS treatment conditions, the mutation efficiency is significantly improved; meanwhile, by carrying out material planting and character observation with single plants and single strains as the unit, the mutant character survey efficiency and accuracy can be significantly improved. The method further has the advantages that the method can be applied to sesame mutant library establishment and directly applied to new variety breeding, various types of mutants can be obtained, and genetic backgrounds are clear and consistent and bring convenience to gene cloning. In general, the method can overcome the technical bottlenecks of good germplasm deficiency, low germplasm creating efficiency and the like in sesame breeding to some extent, and a material foundation can be laid for breeding of good new verities.

Description

technical field [0001] The invention belongs to the technical field of sesame breeding, and specifically relates to a method for large-scale and efficient creation of sesame mutants by using EMS (ethyl methylsulfonate) mutagen. Background technique [0002] Sesame( Sesamum indicum L., 2n=26) belongs to the genus Linaceae, is one of the oldest oil crops in the world, and is also an important characteristic high-quality agricultural product in my country. my country's sesame planting area is about 7 million mu, ranking fourth in the world; the average yield per unit area is 93 kg / mu (2013 national statistics), ranking first in the world, and the average annual total output is 620,000 tons, ranking third in the world , occupies an important position in the world sesame production and international trade. However, compared with grain and other oil crops, sesame is still a low-yielding crop. Therefore, creating and screening new sesame germplasm with high yield, high quality, ...

AI Technical Summary

Problems solved by technology

[0007] In the EMS mutagenesis technology, the EMS mutagen dose, treatment period, and seed activity characteristics all have an important impact on the efficiency of mutagenic material creation, but there are no reports on the EMS mutagenesis efficiency and mutant library construction of sesame.

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