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Monomer stable carbon isotope analysis method for polybrominated diphenyl ether in fish

A technology for stabilizing carbon isotopes and polybrominated diphenyl ethers, which is applied in the field of isotope analysis, can solve problems such as complex sample matrix, and achieve the effect of saving solvent and good purification effect.

Inactive Publication Date: 2016-12-21
SOUTH CHINA INST OF ENVIRONMENTAL SCI MEP
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Problems solved by technology

Therefore, in order to obtain a sufficiently high PBDE content, the experiment requires a large sample volume, which will undoubtedly make the sample matrix very complicated, and more organic substances such as fats and proteins need to be processed

Method used

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  • Monomer stable carbon isotope analysis method for polybrominated diphenyl ether in fish
  • Monomer stable carbon isotope analysis method for polybrominated diphenyl ether in fish
  • Monomer stable carbon isotope analysis method for polybrominated diphenyl ether in fish

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Embodiment Construction

[0021] The technical solution of the present invention will be described in detail below with reference to the accompanying drawings 1-3.

[0022] This example provides the stable carbon isotope analysis method of polybrominated diphenyl ethers in the fish body of the present invention, which requires the following experimental reagents and materials.

[0023] Experimental reagents mainly include: PBDEs industrial product TBDE-71X, PBDEs standard product, distilled water, acetone, dichloromethane, n-hexane, concentrated sulfuric acid, neutral silica gel, anhydrous sodium sulfate, florisil. All solvents were of analytical grade (AR) and double-distilled in an all-glass system before use.

[0024] Alumina: 100-200 mesh, manufactured by China Wuhan Chemical Reagent Company. It was extracted with dichloromethane for 72 hours, and the extract was changed once during the period. After drying in a fume hood, activate at 250°C for 12 hours, cool to room temperature in a desiccator, ...

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Abstract

The invention relates to a monomer stable carbon isotope analysis method for polybrominated diphenyl ether in fish. The monomer stable carbon isotope analysis method is characterized by specifically comprising: 1, extracting a target compound; 2, carrying out first purification on a fish sample; 3, carrying out second purification on the sample; 4, carrying out elution separation on the target compound, wherein the extraction liquid in the step 3 is injected, pre-leaching is performed with 22 mL of n-hexane, and then leaching is performed with 5 mL of n-hexane and 15 mL of dichloromethane-n-hexane having a volume ratio of 1:1 to obtain polybrominated diphenyl ether (PBDEs); 5, completing the quantitative analysis of the polybrominated diphenyl ether (PBDEs); and 6, completing the monomer stable carbon isotope analysis of the polybrominated diphenyl ether (PBDEs). According to the present invention, compared to the commonly used biological sample treatment method, the used experiment method of the present invention has advantages of time saving, solvent saving and good purification effect; and the prepared sample can effectively meet the target compound content required by CSIA analysis so as to easily develop the pollutant source analysis through the CSIA technology.

Description

technical field [0001] The invention relates to an isotope analysis method, in particular to a monomer stable carbon isotope analysis method of polybrominated diphenyl ethers in fish. Background technique [0002] Monomer Isotope Analysis (CSIA) is also known as Gas Chromatography-Isotope Ratio Mass Spectrometry (GC-IRMS). Organic compounds usually have their own stable characteristic isotopic composition when they enter the environment. Under a specific environment, if the isotopic composition of the compound remains unchanged, its isotopic composition can be directly used for source tracing. Due to the influence of certain biological processes in the environment, such as microbial degradation and biological enzyme metabolism, the isotopic composition of some compounds will change regularly. With the continuous development of gas chromatography-isotope ratio mass spectrometry (GC-IRMS), stable isotope analysis (CSIA) of monomeric organic compounds is becoming more and more...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 郑晶曾艳红郑晓波罗孝俊胡国成张丽娟任明忠麦碧娴
Owner SOUTH CHINA INST OF ENVIRONMENTAL SCI MEP
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