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Amino-acylase immobilization carrier and preparation method thereof

A technology of immobilized carrier and aminoacylase, applied in the field of enzyme immobilization, can solve the problems of difficult product separation and purification, unfavorable industrial production, etc.

Inactive Publication Date: 2016-12-21
YINGJING LIHONG TECH PROMOTION SERVICES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problem of difficult separation and purification of products in the prior art, which is not conducive to industrial production, and provides an aminoacylase immobilized carrier, which can be applied to the aminoacylase for the production of amino acids, increasing the stability of the enzyme So as to prolong the service life of the enzyme, improve the enzyme activity, and facilitate the separation and purification of products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] An aminoacylase immobilized carrier, comprising a carrier matrix resin and diethylamine, wherein the carrier matrix resin includes the following raw materials in parts by weight:

[0031] Oil phase:

[0032] Glycidyl methacrylate 30 parts

[0033] 40 parts of divinylbenzene

[0034] Acrylonitrile 5 parts

[0035] 50 parts of toluene

[0036] 50 parts of 2-ethylhexanol

[0037] 1 part of dibenzoyl peroxide;

[0038] water box:

[0039] 5 parts polyvinyl alcohol

[0040] 5 parts gelatin

[0041] NaCl 100 parts

[0042] CaCO 3 15 copies

[0043] 1000 parts of deionized water.

Embodiment 2

[0045] An aminoacylase immobilized carrier, comprising a carrier matrix resin and diethylamine, wherein the carrier matrix resin includes the following raw materials in parts by weight:

[0046] Oil phase:

[0047] Glycidyl methacrylate 40 parts

[0048] 50 parts of divinylbenzene

[0049] Acrylonitrile 10 parts

[0050] 60 parts of toluene

[0051] 60 parts of 2-ethylhexanol

[0052] 3 parts of dibenzoyl peroxide;

[0053] water box:

[0054] 10 parts polyvinyl alcohol

[0055] 10 parts gelatin

[0056] NaCl 200 parts

[0057] CaCO 3 15 copies

[0058] 1000 parts of deionized water.

Embodiment 3

[0060] An aminoacylase immobilized carrier, comprising a carrier matrix resin and diethylamine, wherein the carrier matrix resin includes the following raw materials in parts by weight:

[0061] Oil phase:

[0062] Glycidyl methacrylate 35 parts

[0063] 45 parts of divinylbenzene

[0064] Acrylonitrile 7.5 parts

[0065] 55 parts of toluene

[0066] 55 parts of 2-ethylhexanol

[0067] 1-3 parts of dibenzoyl peroxide;

[0068] water box:

[0069] 7.5 parts of polyvinyl alcohol

[0070] 7.5 parts of gelatin

[0071] NaCl 150 parts

[0072] CaCO 3 15 copies

[0073] 1000 parts of deionized water.

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PUM

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Abstract

The invention relates to an amino-acylase immobilization carrier and a preparation method thereof, and belongs to the technical field of enzyme immobilization. The carrier is prepared from epoxypropyl methacrylate, divinyl benzene, acrylonitrile, methylbenzene, 2-ethyl hexanol, dibenzoyl peroxide, polyvinyl alcohol, gelatin, NaCl, CaCO3 and deionized water. Matrix resin reacts with diethylamine to prepare the amino-acylase immobilization carrier. The activity of an immobilized enzyme obtained by immobilizing amino-acylase through the carrier ranges from 1,000 micromoles per gram to 1,200 micromoles per gram.

Description

technical field [0001] The invention relates to an enzyme-immobilized carrier and a preparation method thereof. More specifically, the invention relates to an aminoacylase-immobilized carrier and a preparation method thereof, belonging to the technical field of enzyme immobilization. Background technique [0002] Liquid enzyme immobilization has many advantages: it can increase the stability of the enzyme and prolong the service life of the enzyme; after the end of the enzyme-catalyzed reaction, the enzyme, the reaction product and the reaction solution can be easily separated by filtration, which not only reduces the operating cost, It also avoids the pollution of the enzyme to the catalytic product; the column operation of the enzyme catalysis can be realized, and the efficiency of the enzyme catalysis can be improved. [0003] At present, the main methods of producing amino acids are microbial fermentation and chemical synthesis. With the development of pharmaceutical in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/08C08L25/00C08L29/04C08L89/00C08F212/36C08F220/32C08F220/44
CPCC12N11/08C08F212/36C08L25/00C08L2205/03C12N9/80C12Y305/01014C08F220/32C08F220/44C08L29/04C08L89/00
Inventor 王有杨景然
Owner YINGJING LIHONG TECH PROMOTION SERVICES CO LTD
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