Microfluidic chip for Raman detection of amphetamin chloride in hair, and use method thereof
A microfluidic chip and methamphetamine technology, which is applied in Raman scattering, chemical instruments and methods, and laboratory containers, can solve the problems of short detection time limit and easy false positives, and achieve reliable and easy-to-preserve detection results , easy to carry and use
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Embodiment 1
[0040] see figure 1 , figure 2 , the device structure of the present invention is as follows:
[0041]The main body is in the shape of a rectangular parallelepiped (length X width: 31.5X15.75mm), and is divided into upper and lower layers, including a substrate A and a cover B, and sealed alignment bonding can be achieved between the two layers.
[0042] The substrate A includes: a labeling solution pool 1 for containing immunogold sol, a washing solution pool 2 for containing buffer solution, a sample reaction and Raman detection area 4, and a waste solution pool 5 for containing stop solution , for the detection liquid pool 6 for containing the immunomagnetic antibody, for the detection liquid pool 7 for the liquid to be tested for hair extraction, wherein:
[0043] The waste liquid pool 5 is located at the rightmost end of the chip, and is connected to the detection area 4 on the left side through the fourth channel 4-1. A magnet is placed on the back of the detection ar...
Embodiment 2
[0050] The preparation process of the nanoparticles labeled with immunogold is as follows;
[0051] Take 49.5ml ultrapure water + 0.5ml HAuCl 3 Stir and disperse evenly, heat and condense under reflux, add 0.5ml Na at one time 3 Cit (trisodium citrate), the color first turned black, purple-black, and finally purple-red, and then condensed and refluxed for 15 minutes to obtain Au sol with a particle size of about 35 nm; take 1 ml of Au sol + 2.5 μl of 1mM MBA, incubate for 1.5 hours, and centrifuge to disperse ; Add 2 μl of 5 μg / ml methamphetamine antigen and incubate for 3.5 h, centrifuge to disperse, and resuspend in 1 ml of phosphate buffer; add 10 μl of 10% BSA to incubate for 1 h, and centrifuge to disperse 1 ml of phosphate buffer.
[0052] The preparation process of the immunomagnetic nanoparticle solution is as follows:
[0053] Take 0.68g FeCl 3 +0.2g Na 3 Cit+20ml EG (ethylene glycol) was dissolved by ultrasonic, and 1.2g CH was added 3 COONa was stirred and mi...
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