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A method for preparing low-molecular-weight polysaccharides

A low-molecular-weight and high-molecular-weight technology, which is applied in the field of preparing low-molecular-weight polysaccharides, can solve problems such as unsuitability for industrialized expanded production, product structure and activity destructiveness, and low degradation rate, and achieve a suitable range of product molecular weight distribution, cheap raw materials, The effect of high extraction rate

Active Publication Date: 2018-10-02
北京颐方生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the cleavage of glycosidic bonds in the acid degradation method is random, and the molecular weight distribution of the product is wide, which is destructive to the structure and activity of the product.
The hydrogen peroxide degradation method requires the use of hydrogen peroxide, which is potentially dangerous during storage and transportation, and has a low degradation rate, so it is not suitable for industrialized expansion of production

Method used

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  • A method for preparing low-molecular-weight polysaccharides
  • A method for preparing low-molecular-weight polysaccharides

Examples

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Effect test

Embodiment 1

[0053] A method for degrading lentinan by using the electric Fenton method: take 1600g lentinan (molecular weight 500KDa), disperse it in 1.6Kg absolute ethanol, add it to 10L distilled water, make a concentration of sugar solution that is 160mg / ml, pack In an electrolytic cell with a volume of 30 L, weigh 85.2 g of sodium sulfate and dissolve it in the polysaccharide solution, and adjust the pH to 3 with 1 mol / L hydrochloric acid. Use 30cm×20cm barbed wire as the anode, 30cm×20cm porous graphite as the cathode, the distance between the two electrodes is 6cm, and 13V direct current is applied, and the current density is 10mA / cm 2 , and use the aeration device to feed in purified air at a speed of 2.0L / min (or to feed in oxygen at a speed of 0.6L / min, the aeration device contains two vent holes, and the two vent holes are blown into oxygen at the same time), per liter The amount of air introduced into the thick lentinan solution is about 24L (the amount of air introduced into t...

Embodiment 2

[0055] A method for degrading lentinan by using the electric Fenton method: take 900g lentinan (molecular weight 500KDa), disperse it in 0.72Kg dehydrated alcohol, add it to 10L distilled water, and make a sugar solution with a concentration of 90mg / ml. In an electrolytic cell with a volume of 30 L, weigh 71 g of sodium sulfate and dissolve it in the polysaccharide solution, and adjust the pH to 3 with 1 mol / L hydrochloric acid. With 20cm×20cm barbed wire as the anode and 20cm×20cm porous graphite as the cathode, the distance between the two electrodes is 5cm, and 13V direct current is applied, and the current density is 12mA / cm 2 , and use the aeration device to feed oxygen at a rate of 0.5L / min. The aeration device contains two vent holes, and the two vent holes are blown into oxygen at the same time. The amount of air per liter of crude lentinan solution is about 4.0L. The stirrer was stirred at a speed of 250rpm. After electrolysis at room temperature for 40 minutes, the p...

Embodiment 3

[0057] A kind of method that adopts electric Fenton method to degrade pachyphyllum: take by weighing 2000g polysaccharide (average molecular weight 452KDa), be dispersed in 2.0Kg dehydrated alcohol, join in 10L distilled water, make the sugar solution that concentration is 200mg / ml, pack into an electrolytic cell with a volume of 30 L, weigh 99.4 g of sodium sulfate and dissolve it in the polysaccharide solution, and adjust the pH to 3 with 1 mol / L hydrochloric acid. Use 30cm×30cm barbed wire as the anode, 30cm×30cm porous graphite as the cathode, the distance between the two electrodes is 5cm, 13V direct current is connected, and the current density is 14mA / cm 2 , and use an aeration device to feed oxygen at a speed of 0.6L / min. The aeration device contains two air holes, and the two air holes are blown into oxygen at the same time. The amount of air introduced into each liter of crude pachyphyllin solution is about 7.2L. The stirrer is stirred at a speed of 250rpm. After ele...

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Abstract

The invention relates to a method for preparing low-molecular-weight polysaccharide. The method comprises the specific steps that a high-molecular-weight polysaccharide solution with the concentration being 40-300mg / mL is introduced into an electrolytic cell provided with a cathode and an anode, and the solution is regulated to be acidic; oxygen is introduced into the cathode with the ratio of oxygen to polysaccharide being (2.0-20.0):1 in liters; and direct current is charged, the cathode current density is controlled to be 5-20mA / cm<2>, degrading is conducted under the conditions, electrolyte is collected, and the low-molecular-weight polysaccharide is obtained. The method provided by the invention is simple in process operation and efficient, saves time and facilitates scale production expanding; in addition, the reaction conditions are moderate, and pollution to the environment is small; and it is important that compared with acid degradation and a hydrogen peroxide degradation method, the polysaccharide degradation rate of an electric Fenton method is high, and the molecular weight range of the obtained low-molecular-weight polysaccharide is narrower.

Description

technical field [0001] The invention relates to the field of production of pharmaceutical raw materials, in particular to a method for preparing low-molecular-weight polysaccharides. Background technique [0002] Polysaccharide is formed by the condensation and dehydration of multiple monosaccharide molecules. It is a kind of carbohydrate with complex molecular structure and huge size. Active polysaccharide refers to a polysaccharide compound with special physiological activity, which has the function of regulating the circadian rhythm of the human body. Studies have shown that the biological activity of polysaccharides is closely related to its molecular weight. The larger the molecular weight, the larger the molecular volume, and the poor solubility. learning activity. Therefore, it is of great value to degrade high-molecular-weight polysaccharides to obtain low-molecular-weight polysaccharides so that they can better exert their biological activities. [0003] The polys...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C25B3/00C25B11/12C25B15/02
CPCC25B3/00C25B15/02C25B11/043
Inventor 石清东王姣
Owner 北京颐方生物科技有限公司
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