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Preservation method and application of soybean virus

A technology of soybean mosaic virus and preservation method, which is applied in the field of preservation of soybean mosaic virus, can solve the problems that it cannot be directly used as a source of virus, the activity of soybean mosaic virus is reduced, time-consuming and laborious, and so on, so as to avoid the interaction between viruses and species Effects of cross-infection, reducing the chance of virus mutation, and improving efficiency

Inactive Publication Date: 2016-12-07
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above-mentioned preservation method can preserve soybean mosaic virus, as the storage time prolongs, especially for more than 1 year, the activity of soybean mosaic virus is significantly reduced, and it cannot be used directly as a source of virus, and the host must be reused (i.e. inoculated with susceptible soybeans). ) to proliferate the virus and restore the infectivity, and then take the young leaves with obvious symptoms as the source of the virus, and it takes at least one and a half months for the host to proliferate the virus once, which is time-consuming and laborious

Method used

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  • Preservation method and application of soybean virus
  • Preservation method and application of soybean virus
  • Preservation method and application of soybean virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1, identification adopts the virus activity of the soybean mosaic virus preserved by different preservation methods

[0060] 1. Soybean mosaic virus materials preserved by different preservation methods

[0061] Material 1: Soybean mosaic virus was inoculated into Nannong 1138-2, and fresh leaves with typical symptoms of soybean mosaic virus were collected and stored at -80°C for 1 year. This material is material 1.

[0062] Material 2: Soybean mosaic virus was inoculated into Nannong 1138-2, and fresh leaves with typical symptoms of soybean mosaic virus were collected and stored at -80°C for 3 years. This material is material 2.

[0063] Material 3: Soybean mosaic virus was inoculated into Nannong 1138-2, and fresh leaves with typical symptoms of soybean mosaic virus were collected and stored at -80°C for 5 years. This material is material 3.

[0064] Material 4: inoculate Nannong 1138-2 with soybean mosaic virus, and collect fresh leaves with typical sympt...

Embodiment 2

[0114] Embodiment 2, utilizing the inoculum prepared in Example 1 to identify the resistance of soybean variety material to soybean mosaic virus

[0115] 1. Materials of soybean varieties to be tested

[0116] The titles of 30 soybean variety materials to be tested and the corresponding disease index (see Table 4) are all recorded in the following documents: Song Yingpei et al., Identification of Soybean Mosaic Virus Adult Plant Resistance and Grain Resistance, Soybean Science, 2015, 34:1015-1019.

[0117] 2. Using the inoculum to identify the disease index of soybean variety materials to soybean mosaic virus

[0118] 1. Planting

[0119] The experiment adopts random block design, with three repetitions in total, and 30 plots in each repetition block, that is, 30 samples of soybean varieties to be tested. All test materials were planted in the experimental farm of the Institute of Oil Crops, Chinese Academy of Agricultural Sciences, and sowed in insect-proof nets. Three ro...

Embodiment 3

[0146] Embodiment 3, utilizing the inoculum prepared in Example 1 to identify the resistance of soybean breeding material to soybean mosaic virus

[0147] 1. Soybean breeding materials to be tested

[0148] The F7 family of Zhongdou 29×Zhongdou 32 was obtained according to the following method (recorded in the following literature: Zhou Xin'an et al., Variation of three and four pods of soybean recombinant inbred lines and its relationship with yield, Chinese Journal of Oil Crops, 2005 , 27:22-25): Zhongdou 29 and Zhongdou 32 were crossed, and the offspring of the hybrids were self-crossed continuously. From the F3 generation, they were planted in separate rows, and each subsequent generation randomly selected a single plant from each row to derive the next generation. Five individual plants were randomly selected from the same family in the F7 generation, the seeds were mixed, and different families were planted separately to obtain a recombinant inbred line population consi...

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Abstract

The invention discloses a preservation method of soybean virus. The preservation method provided by the invention comprises the following steps: a virus preserving fluid is adopted for preserving soybean virus; the virus preserving fluid comprises a polymer in the formula (I) shown in the specification and ascorbic acid; and n represents a natural number ranging from 20 to 70. Experiments prove that the soybean virus preserved by using the preservation method provided by the invention can completely maintain activities within three years under an extra low temperature of -80 DEG C, and can be taken as a drug source for being used directly at any time without need of excitation, the efficiency for identifying the soybean virus is improved greatly, unnecessary subcultures during the multiplication process of the mosaic virus are reduced, mutual cross infection of virus seeds can be avoided, the probability of virus variation is lowered, and the genetic stability of viruses can be kept. Therefore, the preservation method provided by the invention has a significant application value.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preserving soybean mosaic virus and its application. Background technique [0002] Soybean is an important oil crop and food crop in the world, and it is also the source of raw materials for animal husbandry, aquaculture, medicine, light industry, chemical industry and other industries. It plays an important role in the development of my country's national economy, the composition of world food crops and the international oil crop production. status. Soybean mosaic virus disease is a worldwide soybean disease and one of the main soybean diseases in my country. It is distributed in all soybean producing areas. Soybean mosaic virus disease is caused by soybean mosaic virus (Soybeanmosaic virus, SMV). When it infects soybeans, it will lead to a decrease in chlorophyll content and leaf quality in soybean leaves, a decrease in leaf area, and affect photosynthetic area and pho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12Q1/70
CPCC12N7/00C12N2770/34011C12Q1/04
Inventor 陈海峰杨中路单志慧陈李淼张婵娟袁松丽陈水莲邱德珍万乔郝青南张晓娟宫晓敏杨艳艳周新安
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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