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Haemophilus paragallinarum B strain fermentation medium, preparation method thereof and application thereof

A technology of Haemophilus gallinarum and fermentation medium, which is applied in the field of fermentation medium of Haemophilus paragallinarum type B strain, can solve the problems of not being suitable for the growth of type B strains, long adaptation period of bacteria, slow growth, etc., and achieve shortening Bacterial adaptation period, strong bacterial adaptability, and high bacterial content

Active Publication Date: 2016-12-07
山东滨州沃华生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the cultivation process using commercial TSB medium, it was found that the bacteria have a long adaptation period, slow growth, and high cost, which is not suitable for the growth of type B strains

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Medium formula components and ratio:

[0030] Casein peptone 30g, yeast powder 10g, polyvalent peptone 10g, sodium chloride 10g, sodium glutamate 10g, fish meal peptone 8g, glucose 20g, trace element solution 10ml, chicken serum 30ml, coenzyme (NAD solution) 10ml, water , 10M sodium hydroxide solution to adjust pH 7.3.

[0031] Among them, the trace element solution is 2 g of ferrous sulfate heptahydrate, 1.8 g of magnesium chloride, 1.4 g of zinc sulfate heptahydrate, 2.1 g of calcium chloride, 50 g of 36% concentrated hydrochloric acid, and the resulting solution is fixed to 1 L. The concentration of coenzyme (NAD solution) is 1.0g / L.

[0032] The culture medium is prepared by the following method:

[0033] 1) After dissolving casein peptone, yeast powder, polyvalent peptone, NaCl, sodium glutamate, and fish meal peptone, add the trace element solution, add water, dilute to 1000mL, heat up to 37°C, adjust the pH to 7.3, at 121°C Autoclave sterilization for 20 minutes.

[003...

Embodiment 2

[0039] Medium formula components and ratio:

[0040] Casein peptone 15g, yeast powder 8g, polyvalent peptone 8g, sodium chloride 10g, sodium glutamate 10g, fish meal peptone 5g, glucose 15g, trace element solution 50mL, chicken serum 30mL, coenzyme (NAD solution) 10mL, water, 10M Sodium hydroxide solution adjusts the pH to 7.4.

[0041] Among them, the trace element solution is 2 g of ferrous sulfate heptahydrate, 1.8 g of magnesium chloride, 1.4 g of zinc sulfate heptahydrate, 2.1 g of calcium chloride, 50 g of 36% concentrated hydrochloric acid, and the resulting solution is fixed to 1 L. The concentration of coenzyme (NAD solution) is 1.0g / L.

[0042] The medium is prepared by the following method:

[0043] 1) After dissolving casein peptone, yeast powder, polyvalent peptone, NaCl, sodium glutamate, and fish meal peptone, add the trace element solution, add water, dilute to 1000mL, heat up to 37℃, adjust the pH to 7.4, and under 121℃ Autoclave sterilization for 20 minutes.

[0044...

Embodiment 3

[0049] Medium formula components and ratio:

[0050] Casein peptone 15g, yeast powder 5g, polyvalent peptone 5g, sodium chloride 10g, sodium glutamate 10g, fish meal peptone 5g, glucose 15g, trace element solution 5ml, chicken serum 30ml, coenzyme (NAD solution) 5ml, water, 10M Sodium hydroxide solution adjusts the pH to 7.2.

[0051] Among them, the trace element solution is 2 g of ferrous sulfate heptahydrate, 1.8 g of magnesium chloride, 1.4 g of zinc sulfate heptahydrate, 2.1 g of calcium chloride, 50 g of 36% concentrated hydrochloric acid, and the resulting solution is fixed to 1 L. The concentration of coenzyme (NAD solution) is 1.0g / L.

[0052] The medium is prepared by the following method:

[0053] 1) After dissolving casein peptone, yeast powder, polyvalent peptone, NaCl, sodium glutamate, and fish meal peptone, add the trace element solution, add water, dilute to 1000 mL, heat up to 37°C, adjust the pH to 7.4, and adjust the pH to 121°C Autoclave sterilization for 20 min...

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PUM

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Abstract

The invention discloses a haemophilus paragallinarum B strain fermentation medium, a preparation method thereof and application thereof, and belongs to the field of agricultural microorganism. The haemophilus paragallinarum B strain fermentation medium is prepared from 5 to 100 g / L of casein peptone, 0.5 to 80 g / L of yeast powder, 0.5 to 80 g / L of polyvalent peptone, 0.5 to 20 g / L of sodium chloride, 0.5 to 20 g / L of sodium glutamate, 0.5 to 30 g / L of glucose, 0.5 to 30 g / L of fish meal peptone, 5 to 100 mL / L of chicken serum, 0.5 to 50 mL / L of microelement solution and 1 to 30 mL / L of NAD (Nicotinamide Adenine Dinucleotide) solution. The haemophilus paragallinarum B strain fermentation medium disclosed by the invention contains all nutritional ingredients which are needed for growth of haemophilus paragallinarum B strains, growth and metabolism of the strains are facilitated, the adaption period of thallus is shortened, the adaptive capacity of the thallus after inoculation is strong, the fermentation period is short, the viable bacteria quantity during harvesting is high, and the haemophilus paragallinarum B strain fermentation medium is suitable for large-scale application and has a wide prospect.

Description

Technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a fermentation medium for a type B strain of Haemophilus paragallinarum. Background technique [0002] As the pathogenic bacteria of infectious rhinitis in chickens, Haemophilus paragallinarum can cause upper respiratory tract infections in chickens. When the disease breaks out, it can cause a decrease in egg production of laying hens, hinder the growth and development of pregnant and adult chickens and increase the elimination rate, which is beneficial to the chicken industry. Cause greater losses, etc. [0003] According to Page's typing method, Haemophilus paragallinarum can be divided into three serotypes: A, B, and C, and there is no cross-protection between each type. According to epidemiological investigations, the prevalence of type B strains is wide. [0004] At present, there are many research reports on type A in the literature, and certain results have be...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/21
Inventor 朱杰王楠牛成明董新荣
Owner 山东滨州沃华生物工程有限公司
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