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Tissue culture and rapid propagation method of seed germination way of semiliquidambar cathayensis

A technique for tissue culture rapid propagation and semi-maple lotus, applied in the field of plant tissue culture

Inactive Publication Date: 2016-12-07
YULIN NORMAL UNIVERSITY
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Problems solved by technology

[0009] 3. Insufficiency of existing tissue culture technology
In recent years, research on the medicinal ingredients and value of Banfenghe has been increasing. At present, there is no report on the sterile tissue culture of the complete process of Banfenghe from seed germination to multiplication and rooting of strong seedlings. In terms of rapid propagation of bacteria culture, stems and leaves are often used as explants to induce

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  • Tissue culture and rapid propagation method of seed germination way of semiliquidambar cathayensis
  • Tissue culture and rapid propagation method of seed germination way of semiliquidambar cathayensis
  • Tissue culture and rapid propagation method of seed germination way of semiliquidambar cathayensis

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Embodiment 1

[0068] Tissue Culture of a Seed Germination Pathway of Scutellaria chinensis

[0069] (1) Germination and cultivation of the seeds of the maple lotus: take the mature wild maple lotus capsules from Naqin, Fangchenggang, Guangxi, dry the mature capsules of the maple lotus seeds until they are 90% dry, and take out the seeds as explant raw materials; Clean the selected seeds with detergent, place them on an ultra-clean workbench, soak them in 0.1% sodium hypochlorite stock solution for 5-7 minutes, and then rinse them with sterile water for 5-8 times; disinfect the explants and inoculate them in In a sterile induction medium, cultured under the conditions of temperature 25-27°C, light intensity 1500-2000lx, and light time 10 hours / day, the germination rate of seeds reached 77% in 15 days, and 93% after 30 days . See attached figure 1 with figure 2 .

[0070] The seed germination medium is:

[0071] B1: Improved MS+GA 3 0.2mg / L+VB 2 1.0mg / L+sugar 30g / L+agar 3.6g / L;

[00...

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Abstract

The invention discloses a tissue culture and rapid propagation method of a seed germination way of semiliquidambar cathayensis. The tissue culture and rapid propagation method comprises the following steps of step 1, making sterile materials; step 2, performing seed germination culture; step 3, inducing callus tissue; step 4, performing callus tissue proliferation; step 5, performing callus tissue differentiation; step 6, inducing multiple shoots; step 7, cutting off base parts of the differentiated multiple shoots obtained in the step 6, and inoculating the differentiated multiple shoots without the base parts into culture mediums so as to obtain sound seedlings; and step 8, cutting off base parts of the sound seedlings obtained in the step 7, inoculating the sound seedlings without the base parts into the culture mediums, and performing rooting culture. The invention aims to provide a complete tissue culture method for the semiliquidambar cathayensis, which is rapid, high in proliferation rate and high in survival rate.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a tissue culture and rapid propagation method for the germination pathway of the seeds of the maple lotus. Background technique [0002] Semiliquidambar cathayensis H.T.Chang is a plant of the genus Semiliquidambar cathayensis H.T.Chang. It is the type species of a new genus Semiliquidambar cathayensis published in 1962 and is endemic to my country. This species has extremely high value in many aspects such as academic research, traditional Chinese medicine and garden application. However, due to unreasonable development and utilization, its resources have been severely damaged. In 1987, the State Environmental Protection Bureau and other promulgated the "China Rare and Endangered Protected Plant List (Volume 1)", it was listed as a national third-level key protected plant [2] ; Listed as a rare species in the "Chinese Plant Red Book - Rare and Endangered Plants" e...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 黄肇宇梁伟江卢海啸韦小红陈柑宏
Owner YULIN NORMAL UNIVERSITY
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